LOCUS       sequence-25546-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, 
            Florens L, Conaway RC, Cohen RE, Conaway JW
  TITLE     Distinct modes of regulation of the Uch37 deubiquitinating enzyme in
            the proteasome and in the Ino80 chromatin-remodeling complex.
  JOURNAL   Mol Cell. 2008 Sep 26. 31(6):909-17.
  PUBMED    18922472
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Sep 26. 31(6):909-17."
COMMENT     Sequence Label: pcDNA5 HA-Arp8
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tggggnntta gcgagctctc tggctactga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct ggctagccgc caccatggct tacccatacg
      121 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tctctcgaga
      181 tgacccaggc tgagaagggt gatacggaga acggaaagga gaagggcggc gagaaggaga
      241 aggagcagcg cggcgtgaag cggcccatcg tgcccgcgct ggtgccggag tcgctgcaag
      301 agcaaatcca gagcaacttc atcattgtca tacatccagg ttcaacaact ttaaggattg
      361 gtcgagccac agacactctt cctgccagca ttcctcacgt cattgcccga agacacaaac
      421 aacaagggca gcccctatac aaggacagtt ggctcctaag ggagggacta aataaaccag
      481 aaagtaatga acaaagacaa aatggcctta aaatggtgga tcaagcaata tggtctaaaa
      541 agatgtccaa tggtacaaga cgcattcctg tgtcccctga acaggcacgc tcctacaata
      601 agcagatgcg acctgcaatt ttagatcact gttcgggaaa taagtggaca aacacatctc
      661 atcaccctga gtatttagta ggagaagagg ccttgtatgt taatccactg gactgttacn
      721 attnntccnn ggcctaaagn nnnnnaaggt ttnattttcc cncggggnct tgntgttt
//
