LOCUS       sequence-255489-       11523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: MLKP1delTM
FEATURES             Location/Qualifiers
     source          1..11523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2096..2120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2492..2671
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2707..3732
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3930..4182
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4275..4299
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4820..5611
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5857..6445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6346..6365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6599..6616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6631..6771
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6697..6716)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6857..6879
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7115..7309
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7375..8448)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8877..9506)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10807..10831
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccgatcctgg tggagctgga cggggatgtt
      541 aacggccaca agttctcagt gtccggcgag ggggagggcg acgccaccta cgggaagctc
      601 acgctgaagt tcatttgcac gaccggcaag ctgccggtgc cctggccaac cctggtcaca
      661 actctgacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaagt ccgctatgcc agagggctac gtgcaggagc gcacgatctt cttcaaggac
      781 gatggcaact acaagaccag ggccgaggtc aagttcgagg gggacacgct cgttaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata tcctcggcca caagctggag
      901 tacaactaca attcgcataa cgtctacatc atggcggaca agcagaagaa cgggattaag
      961 gttaatttca agatccggca caatattgag gacggcagcg tgcagctcgc tgatcattac
     1021 cagcagaaca caccgatcgg ggacggccct gtcctcctgc ccgataatca ctacctctct
     1081 actcagtcag cgctgtccaa ggacccgaac gagaagcgcg atcatatggt cctcctggag
     1141 ttcgttacag ccgcggggat cactctcggc atggacgagc tgtacaaggg ggctggcgat
     1201 tacaaggacg atgacgataa ggcctacccc tacgacgtgc cagattacgc cggggctggc
     1261 atgagggcgg gcgacgcgtt cagcaactac cgctgcagga ccatgtcgat gaattacgac
     1321 ggcgatagga gcgaggatcg gcagtcggag gaggtgcagt ctgcctacaa gcgggacagc
     1381 gatgtcggcg aggagatgat catttccagc gaggagcaga acgacgagaa tgagtggagc
     1441 tgggtcaacc aggaccggaa tggcgatccc ttcgcggagt cggtttcgtc tctccacacc
     1501 acgcagcaga tcctggagaa tgagattcgc aagctgtcgg agctggggaa ggagctggag
     1561 gcggaggaga gcacctcggg caacaaggac caggatgtta tcctcctgcc atactctgag
     1621 gccgacgtgc tggagctgtc agagaagatg gagcacctgg agcagaaggt caaggaggcg
     1681 tcgaacacca ttcgcgagaa ggacctcatg ctgtctaagc tgcagatcct cattggcacg
     1741 gcggatcggg ctgccctgga ggaggagctg gaggaggccg ctgcttccat cgaccagctg
     1801 gagacggagc tggagcgcca cctccaggag aagctggagg cggagattca gtgcctcctg
     1861 atgctcaagg cttcccagaa ctggcaggtt cgcgcggagg acagggtggc cctggaggag
     1921 catagggctc tgtctgccgg cgataagaca cggatgctcc tgaagctgcg cgagactgag
     1981 agcaagatcg tcatgctcaa ggagcaggtg gacaagctgg aggtcaggga gaaggagctg
     2041 aaccggacaa ctgaggtgct gaagatgcag tcacggacat tccgcgcgtc ctgagaccca
     2101 gctttcttgt acaaagtggt gatatcccgc ggccatgcta gagtccgcaa aaatcaccag
     2161 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     2221 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     2281 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     2341 aaaatccagt gacctgcagg catgcgacgt cgggccctct agaggatccc cgggtaccgc
     2401 gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     2461 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc
     2521 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     2581 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     2641 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aactcgaatc
     2701 ccccctatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     2761 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     2821 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     2881 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     2941 gacattgggg agttcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     3001 acgttgcaag acctgcctga aaccgaactg cccgctgttc ttcagccggt cgcggaggct
     3061 atggatgcta tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     3121 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     3181 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     3241 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     3301 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     3361 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     3421 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     3481 tcgccaaggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     3541 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     3601 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     3661 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     3721 gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg cctcagccag
     3781 caactcgcgc gagcctagca aggcaaatgc gagagaacgg ccttacgctt ggtggcacag
     3841 ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc gggagggccg gtcgcagtga
     3901 ttcaggaatt aattccctag agtcaagcag atcgttcaaa catttggcaa taaagtttct
     3961 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4021 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4081 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4141 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     4201 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     4261 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     4321 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     4381 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     4441 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     4501 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     4561 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     4621 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     4681 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     4741 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     4801 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     4861 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     4921 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     4981 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     5041 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     5101 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     5161 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     5221 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     5281 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     5341 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     5401 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     5461 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     5521 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     5581 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     5641 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     5701 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     5761 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6001 tgtycttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6541 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6601 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     6661 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6721 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     6781 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6841 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6901 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     6961 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     7021 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     7081 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     7141 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     7201 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     7261 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     7321 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     7381 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     7441 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     7501 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     7561 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     7621 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     7681 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     7741 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     7801 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     7861 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     7921 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     7981 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     8041 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     8101 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     8161 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     8221 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     8281 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     8341 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     8401 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     8461 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     8521 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     8581 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     8641 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     8701 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     8761 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     8821 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     8881 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     8941 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9001 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     9061 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     9121 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9181 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9241 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9301 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9361 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9421 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9481 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     9541 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     9601 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
     9661 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
     9721 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
     9781 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
     9841 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
     9901 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
     9961 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10021 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    10081 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    10141 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10201 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10261 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    10321 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    10381 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    10441 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    10501 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    10561 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    10621 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    10681 swtyaaagta ctttrawsym swkkrrarcc ctgtggttgg catgcacata caaatggacg
    10741 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    10801 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga
    10861 aacgacaatc tgatccaagc tcaagctaag cttgagctct cccatatggt cgactagagc
    10921 caagctgatc tcctttgccc cggagatcac catggacgac tttctctatc tctacgatct
    10981 aggaagaaag ttcgacggag aaggtgacga taccatgttc accaccgata atgagaagat
    11041 tagcctcttc aatttcagaa agaatgctga cccacagatg gttagagagg cctacgcggc
    11101 aggtctcatc aagacgatct acccgagtaa taatctccag gagatcaaat accttcccaa
    11161 gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga
    11221 tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcataa
    11281 accaaggcaa gtaatagaga ttggagtctc taagaaagta gttcctactg aatcaaaggc
    11341 catggagtca aaaattcaga tcgaggatct aacagaactc gccgtgaaga ctggcgaaca
    11401 gttcatacag agtcttttac gactcaatga caagaagaaa atcttcgtca acatggtgga
    11461 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
    11521 tat
//
