LOCUS       sequence-255490-        9928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with 
            mNeonGreen reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso 
            CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, 
            Lindquist S, Khalil AS
  TITLE     A Genetic Tool to Track Protein Aggregates and Control Prion 
            Inheritance.
  JOURNAL   Cell. 2017 Nov 2;171(4):966-979.e18. doi: 
            10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.
  PUBMED    29056345
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.041"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "966-979.e18"
COMMENT     Sequence Label: pSK226
FEATURES             Location/Qualifiers
     source          1..9928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     protein_bind    961..985
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2207..2231)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2269..2289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2893..2982
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             2989..3078
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      4149..4158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      4625..4812
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4680..4704)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             5236..6045
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5303..5322)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6562..6578
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6562..6578
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6592..6611)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6593..6611)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6618..6635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6618..6634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6627..6649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6934..6953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7066..7087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7241..7260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7360..7382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7420..7438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7506..7610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7611..7679,7680..8471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7829..7848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8642..9230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9131..9150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9384..9401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9518..9539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    9594..9610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9599..9621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9618..9634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9618..9634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta
       61 tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat
      121 gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa
      181 aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga
      241 agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac
      301 ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt
      361 tacatttcag caatatatat atatatattt caaggatata ccattctaat gtctgcccct
      421 aagatggcgt cgtacgctgc accgagtgca accacacaaa aatcatacaa cgaatggtat
      481 atgcttcatt tctttgtttc gcattagctg cgctatttga ctcaaattat tattttttac
      541 taagacgacg cgtcacagtg ttcgagtctg tgtcatttct tttgtaattc tcttaaacca
      601 cttcataaag ttgtgaagtt catagcaaaa ttcttccgca aaaagatgaa tcttagttct
      661 cagcccacca aaagaggtac atgctaagat catacagaag ttattgtcac ttcttacctt
      721 gctcttaaat gtacattaca accgggtatt atatcttaca tcatcgtata atatgatctt
      781 tctttatgga gaaaattttt ttttcactcg accaaagctc ccattgcttc tgaagagtgt
      841 agtgtatatt ggtacatctt ctcttgaaag actccattgt actgtaacaa aaagcggttt
      901 cttcatcgac ttgctcggaa taacatctat atctgcccac tagcaacagc tagccccatc
      961 acaagtttgt acaaaaaagc aggctacaaa atggatacgg ataagttaat ctcagaggct
     1021 gagtctcatt tttctcaagg aaaccatgca gaagctgttg cgaagttgac atccgcagct
     1081 cagtcgaacc ccaatgacga gcaaatgtca actattgaat cattaattca aaaaatcgca
     1141 ggatacgtca tggacaaccg tagtggtggt agtgacgcct cgcaagatcg tgctgctggt
     1201 ggtggttcat cttttatgaa cactttaatg gcagactcta agggttcttc ccaaacgcaa
     1261 ctaggaaaac tagctttgtt agccacagtg atgacacact catcaaataa aggttcttct
     1321 aacagagggt ttgacgtagg gactgtcatg tcaatgctaa gtggttctgg cggcgggagc
     1381 caaagtatgg gtgcttccgg cctggctgcc ttggcttctc aattctttaa gtcaggtaac
     1441 aattcccaag gtcagggaca aggtcaaggt caaggtcaag gtcaaggaca aggtcaaggt
     1501 caaggttctt ttactgcttt ggcgtctttg gcttcatctt tcatgaattc caacaacaat
     1561 aatcagcaag gtcaaaatca aagctccggt ggttcctcct ttggagcact ggcttctatg
     1621 gcaagctctt ttatgcattc caataataat cagaactcca acaatagtca acagggctat
     1681 aaccaatcct atcaaaacgg taaccaaaat agtcaaggtt acaataatca acagtaccaa
     1741 ggtggcaacg gtggttacca acaacaacag ggacaatctg gtggtgcttt ttcctcattg
     1801 gcctccatgg ctcaatctta cttaggtggt ggacaaactc aatccaacca acagcaatac
     1861 aatcaacaag gccaaaacaa ccagcagcaa taccagcaac aaggccaaaa ctatcagcat
     1921 caacaacagg gtcagcagca gcaacaaggc cactccagtt cattctcagc tttggcttcc
     1981 atggcaagtt cctacctggg caataactcc aattcaaatt cgagttatgg gggccagcaa
     2041 caggctaatg agtatggtag accgcaacag aatggtcaac agcaatccaa tgagtacgga
     2101 agaccgcaat acggcggaaa ccagaactcc aatggacagc acgaatcctt caatttttct
     2161 ggcaactttt ctcaacagaa caataacggc aaccagaacc gctacaaccc agctttcttg
     2221 tacaaagtgg tgatgggctg caggaattcg atatcaagct taatggctcc aaagaagaag
     2281 agaaaggttg gtattcatgg tgttccagtt gatggtggtt tacaagctcc accaactgat
     2341 gtttctttgg gtgatgaatt gcatttggat ggtgaagatg ttgctatggc tcatgctgat
     2401 gctttggatg attttgattt ggatatgttg ggtgacggtg attctccagg tccaggtttt
     2461 actccacatg attctgctcc atatggtgct ttagatatgg ctgactttga attcgaacaa
     2521 atgttcaccg atgccttggg tattgatgaa tatggtggtg gtgctagacc cggggagcgc
     2581 cccttccagt gtcgcatttg catgcggaac ttttcgagaa atttcatttt gcaaagacat
     2641 acccgtactc ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc
     2701 gatagagcta atttgagaag acatctacgt acgcacaccg gcgagaagcc attccaatgc
     2761 cgaatatgca tgcgcaactt cagtagacat gatcaattga ctagacacct aaaaacccac
     2821 ctgagaggtt ctgccggcga attggctttg aaattggctg gtttggatat caattctggt
     2881 tctgctgcta gatacccata tgatgttcca gattatgctg gttaccctta cgatgttcct
     2941 gactacgcag gttcttatcc atacgatgta cctgattatg cctcaagata tccttatgac
     3001 gtccctgatt acgccggtta tccatatgac gtaccagact acgctggtag ttacccatac
     3061 gacgtcccag attacgcttc ttcataatga gggccctcat gtaatttctt gcaaacataa
     3121 gtaaatgcaa acacaataat accgatcata aagcattttc ttctatatta aaaaacaagg
     3181 tttaataaag ctgttatata tatatatata tatatagacg tataattagt ttagttcttt
     3241 ttgtaccata taccataaac aaggtaaact tcacctctca atatatctag aatttcataa
     3301 aaatatctag caaggtttca actccttcaa tcacgttttc atcactcaac cctatctcgg
     3361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3421 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat
     3481 gcggtatttt ctttaattaa tgaggacgtg tagtttaatt gaggacgtgt ggttctaaag
     3541 tgaggacgtg tattaaatat gaggacgtgt ttaaagcatt gaggacgtgt cctaaatctg
     3601 aggacgtgta attatctgag gacgtgtttt agaagtgagg acgtgtggcc ggcccagatc
     3661 cgccaggcgt gtatatatag cgtggatggc caggcaactt tagtgctgac acatacaggc
     3721 atatatatat gtgtgcgacg acacatgatc atatggcatg catgtgctct gtatgtatat
     3781 aaaactcttg ttttcttctt ttctctaaat attctttcct tatacattag gacctttgca
     3841 gcataaatta ctatacttct atagacacac aaacacaaat acacacactg tttaaacaaa
     3901 aaatgggcga ggaggataac atggcctctc tcccagcgac acatgagtta cacatctttg
     3961 gctccatcaa cggtgtggac tttgacatgg tgggtcaggg caccggcaat ccaaatgatg
     4021 gttatgagga gttaaacctg aagtccacca agggtgacct ccagttctcc ccctggattc
     4081 tggtccctca tatcgggtat ggcttccatc agtacctgcc ctaccctgac gggatgtcgc
     4141 ctttccaggc cgccatggta gatggctccg gataccaagt ccatcgcaca atgcagtttg
     4201 aagatggtgc ctcccttact gttaactacc gctacaccta cgagggaagc cacatcaaag
     4261 gagaggccca ggtgaagggg actggtttcc ctgctgacgg tcctgtgatg accaactcgc
     4321 tgaccgctgc ggactggtgc aggtcgaaga agacttaccc caacgacaaa accatcatca
     4381 gtacctttaa gtggagttac accactggaa atggcaagcg ctaccggagc actgcgcgga
     4441 ccacctacac ctttgccaag ccaatggcgg ctaactatct gaagaaccag ccgatgtacg
     4501 tgttccgtaa gacggagctc aagcactcca agaccgagct caacttcaag gagtggcaaa
     4561 aggcctttac cgatgtgatg ggcatggacg agctgtacaa gtaaggcgcg ccacttctaa
     4621 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     4681 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     4741 ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca
     4801 cacctctacc ggcagatccg ctagggataa cagggtaata tagatcgttt agcttgcctc
     4861 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
     4921 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
     4981 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
     5041 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
     5101 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
     5161 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
     5221 tcaattgaaa caaccatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc
     5281 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     5341 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     5401 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     5461 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     5521 accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt
     5581 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     5641 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     5701 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     5761 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     5821 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     5881 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     5941 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     6001 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaaagatc ttcagtactg
     6061 acaataaaaa gattgcttaa aaagattctc tttttttatg atatttgtac ataaacttta
     6121 taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt tcatagggta
     6181 gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa
     6241 aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca
     6301 ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa
     6361 atcatgaaac tatgattcct aatttatata ttggaggatt ttctctaaaa aaaaaaaaat
     6421 acaacaaata aaaaacactc aatgacctga ccatttgatg gagtttaagt caatacctcc
     6481 ttgaaccatt tcccataatg gtgaaagttc cctcaagaat tttactctgt cagaaacggc
     6541 cttaacgacg taggcggccg ctctagaact agtggatcga tccccaattc gccctatagt
     6601 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6661 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6721 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     6781 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     6841 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6901 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     6961 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggtgggcca tcgccctgat
     7021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7081 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     7141 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7201 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     7261 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7321 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7381 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     7441 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9421 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9481 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9541 aggcacccca ggctttacac tttatgcttc cgcggctcgt atgttgtgtg gaattgtgag
     9601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccacta gtccgaggcc
     9661 tcgagatccg atatcgccgt ggcggccgcc gaggagaact tctagtatat ctacatacct
     9721 aatattattg ccttattaaa aatggaatcc caacgattac atcaaaatcc acattctctt
     9781 caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg
     9841 ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc
     9901 tgattcaaga aatatcttga ccgcagtt
//
