LOCUS       sequence-255492-        8457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for multivalent scaffold of 
            (SUMO)10-(SIM)6 biomolecular condensates to recruit SIM-containing 
            client proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK
  TITLE     Compositional Control of Phase-Separated Cellular Bodies.
  JOURNAL   Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. 
            Epub 2016 Jun 30.
  PUBMED    27374333
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.06.010"; journalName: "Cell"; date: 
            "2016-07-28- 28"; volume: "166"; issue: "3"; pages: "651-663"
COMMENT     Sequence Label: RFP-(SUMO)10-(SIM)6
FEATURES             Location/Qualifiers
     source          1..8457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3857..3978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3894..3913)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3948..3967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3985..4440)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4122..4143)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4334..4353
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4467..4571
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4573..4930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4573..4764
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4596..4616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4781..4916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4965..5759
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4965..5759
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5019..5038)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5629..5648
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5947..5966)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5991..6038
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6367..6955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6856..6875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7130..7433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7434..7637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7588..7608
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7676..7685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7682..8389
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7827..7845)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8095..8114
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8289..8312
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaaaaccgag aatgaccaca ttaacttgaa ggttgctgga caggatgggt ccgtggttca
       61 gttcaaaatt aaacgccaca caccgttgtc gaaactgatg aaagcgtact gcgaacgcca
      121 gggtctttct atgcgtcaga ttcgcttccg ctttgatggt caaccgatta atgaaacgga
      181 cacgccggca cagctggaga tggaggatga agacactatc gacgtatttc agcagcagac
      241 cgtcgtaggg ggctccggcg gaagcggggg gtcgggaggt tccatgagcg aagagaaacc
      301 aaaagaagga gttaaaaccg aaaacgacca tatcaacctg aaggtcgccg gtcaggacgg
      361 ctcagtcgtg cagttcaaga ttaaacgtca taccccatta agtaagctga tgaaggcgta
      421 ttgtgagcgc caaggcttgt cgatgcgcca gattcgtttt cgctttgatg gtcagccaat
      481 caacgaaacc gacaccccgg cgcagctgga aatggaagat gaagatacca tcgatgtgtt
      541 ccaacagcaa acggttgttg gcggaagcgg cgggagcggt ggtagcgggg gttctatgtc
      601 cgaagaaaaa ccgaaagagg gtgtgaagac tgaaaacgac cacattaatc tgaaggtggc
      661 gggccaggac ggttccgttg ttcagtttaa aatcaaacgt cacaccccgc tgtcgaaact
      721 gatgaaagcc tattgcgaac gtcaagggct gagcatgcgt caaattcgtt ttcgctttga
      781 tggtcagcca atcaacgaaa ccgatacacc ggcgcagctg gagatggaag acgaggacac
      841 cattgacgtc ttccagcaac aaactgtagt gggtggctcc ggtggctctg gtgggtctgg
      901 tggctcgatg agtgaggaaa aacccaaaga aggcgtcaag actgagaacg atcatattaa
      961 cctgaaagtc gcgggtcaag atggttcagt ggtgcagttc aaaattaaac gccacacccc
     1021 gctgtcaaag ctgatgaaag cctattgtga gcgtcaaggt ctgtcgatgc gccagatccg
     1081 ctttcgcttt gatggtcagc caatcaacga gacagacact ccggcacaac tggagatgga
     1141 agatgaagac accatcgatg tttttcaaca gcagaccgtg gtgggaggga gcggcggtag
     1201 cgggggatca ggtggtagta tgtctgagga aaagccgaag gaaggggtaa agaccgaaaa
     1261 tgatcacatt aatctgaaag ttgcgggcca agatggtagt gtagtgcaat ttaaaatcaa
     1321 gcgtcacacc ccgttatcta aactgatgaa agcatattgc gaacgccaag gcctgtctat
     1381 gcgccagatt cgctttcgct ttgacgggca accaattaat gaaacggaca ccccggcaca
     1441 actggaaatg gaagacgagg atactattga cgtgtttcag caacagacag tggtcggcgg
     1501 ctccggtgga tcaggtggga gtggagggtc tatgtcagaa gagaagccta aggagggcgt
     1561 gaaaaccgag aatgaccaca ttaacttgaa ggttgctgga caggatgggt ccgtggttca
     1621 gttcaaaatt aaacgccaca caccgttgtc gaaactgatg aaagcgtact gcgaacgcca
     1681 gggtctttct atgcgtcaga ttcgcttccg ctttgatggt caaccgatta atgaaacgga
     1741 cacgccggca cagctggaga tggaggatga agacactatc gacgtatttc agcagcagac
     1801 cgtcgtaggg ggctccggcg gaagcggggg gtcgggaggt tccatgagcg aagagaaacc
     1861 aaaagaagga gttaaaaccg aaaacgacca tatcaacctg aaggtcgccg gtcaggacgg
     1921 ctcagtcgtg cagttcaaga ttaaacgtca taccccatta agtaagctga tgaaggcgta
     1981 ttgtgagcgc caaggcttgt cgatgcgcca gattcgtttt cgctttgatg gtcagccaat
     2041 caacgaaacc gacaccccgg cgcagctgga aatggaagat gaagatacca tcgatgtgtt
     2101 ccaacagcaa acggttgttg gcggaagcgg cgggagcggt ggtagcgggg gttctatgtc
     2161 cgaagaaaaa ccgaaagagg gtgtgaagac tgaaaacgac cacattaatc tgaaggtggc
     2221 gggccaggac ggttccgttg ttcagtttaa aatcaaacgt cacaccccgc tgtcgaaact
     2281 gatgaaagcc tattgcgaac gtcaagggct gagcatgcgt caaattcgtt ttcgctttga
     2341 tggtcagcca atcaacgaaa ccgatacacc ggcgcagctg gagatggaag acgaggacac
     2401 cattgacgtc ttccagcaac aaactgtagt gggtggctcc ggtggctctg gtgggtctgg
     2461 tggctcgatg agtgaggaaa aacccaaaga aggcgtcaag actgagaacg atcatattaa
     2521 cctgaaagtc gcgggtcaag atggttcagt ggtgcagttc aaaattaaac gccacacccc
     2581 gctgtcaaag ctgatgaaag cctattgtga gcgtcaaggt ctgtcgatgc gccagatccg
     2641 ctttcgcttt gatggtcagc caatcaacga gacagacact ccggcacaac tggagatgga
     2701 agatgaagac accatcgatg tttttcaaca gcagaccgtg gtgggaggga gcggcggtag
     2761 cgggggatca ggtggtagta tgtctgagga aaagccgaag gaaggggtaa agaccgaaaa
     2821 tgatcacatt aatctgaaag ttgcgggcca agatggtagt gtagtgcaat ttaaaatcaa
     2881 gcgtcacacc ccgttatcta aactgatgaa agcatattgc gaacgccaag gcctgtctat
     2941 gcgccagatt cgctttcgct ttgacgggca accaattaat gaaacggaca ccccggcaca
     3001 actggaaatg gaagacgagg atactattga cgtgtttcag caacagacag tggtcggcgg
     3061 ctccggtgga tcaggtggct ctggtgggag caaagtagat gtgatcgact tgaccatcga
     3121 aagcagcagc gacgaagaag aagatccgcc tgccaaacgc ggcggtagcg gtggctccgg
     3181 tggctctggt gggagcaaag tagatgtgat cgacttgacc atcgaaagca gcagcgacga
     3241 agaagaagat ccgcctgcca aacgcggcgg tagcggtggc tctggcggga gcggcggctc
     3301 gaaagttgat gtcattgacc ttacaattga atcttcgagc gatgaagagg aagatcctcc
     3361 agcgaaacgt ggcggctcgg gaggttcggg cggcagcggc ggaagcaaag tcgatgtaat
     3421 tgacttaacg atcgaatcta gcagcgatga agaagaagat ccaccggcta aacgtggggg
     3481 ctcagggggt tctggcggtt caggcggcag taaagttgac gtgattgacc tcaccatcga
     3541 aagcagtagc gacgaggaag aagatccacc agcaaaacgc ggagggagcg ggggcagcgg
     3601 cggcagcggc ggatctaaag tggacgttat cgatctgacg atcgaaagca gtagcgatga
     3661 ggaagaggac ccgccggcga aacggggcgg ctcgggggga tcttaggtcg acggtaccgc
     3721 gggcccggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     3781 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3841 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3901 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3961 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     4021 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4081 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4141 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4201 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4261 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4321 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4381 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4441 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4501 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4561 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     4621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4861 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4921 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4981 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5041 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5101 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     5161 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5221 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5281 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5341 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5401 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5461 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     5521 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5581 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5641 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5701 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     5761 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     5821 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5881 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     5941 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     6001 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     6061 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     6121 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     6181 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     6241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6481 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6541 ccacttcaag aactctgtat caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
     7081 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7141 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7201 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7261 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7321 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7381 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7441 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7501 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7561 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7621 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     7681 catggtgagc aagggcgagg aggacaacat ggccatcatc aaggagttca tgcgcttcaa
     7741 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     7801 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     7861 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     7921 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     7981 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     8041 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     8101 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     8161 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     8221 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     8281 caacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     8341 gcgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggaggctc
     8401 cggcgggagc ggtgggagtg gagggtctat gtcagaagag aagcctaagg agggcgt
//
