LOCUS       sequence-25550-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(177..197)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(177..195)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(216..232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(216..232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(229..251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 agattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgntt taaacaccac
       61 ttcctgtcag ttaacttttt gaattctttg ttaggcctgg tacctctaga gatatcctgc
      121 agtggccaat gcattggttc gcggccgcca ccgcggtgga gctccagctt ttgttccctt
      181 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      241 tgttatccgc tcgggccctg ccatagcctc aggttactca tatatacttt agattgattt
      301 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      421 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      481 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      541 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      721 accggatagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagctgganc
      781 gacgactaca ccgactgaan actacagcgt gagctnnacg aac
//
