LOCUS       sequence-255519-       10359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal HIV-1 packaging plasmid for gag and pol expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Berthoux L, Sebastian S, Sokolskaja E, Luban J
  TITLE     Lv1 inhibition of human immunodeficiency virus type 1 is 
            counteracted by factors that stimulate synthesis or nuclear 
            translocation of viral cDNA.
  JOURNAL   J Virol. 2004 Nov;78(21):11739-50. doi: 
            10.1128/JVI.78.21.11739-11750.2004.
  PUBMED    15479815
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.78.21.11739-11750"; journalName: "J Virol"; date: 
            "2004-11"; volume: "78"; issue: "21"; pages: "11739-50"
COMMENT     Sequence Label: p8.9 NdeltaSB
FEATURES             Location/Qualifiers
     source          1..10359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        62..365
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        366..569
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     520..540
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     566..590
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             844..2346
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2139..5150
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4835..4952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5724..5957
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6142..6186
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6480..6506
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(7129..7147)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7130..7147)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7437..7456)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7670..7689)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7789..7811
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        7935..8039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8040..8108,8109..8900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8258..8277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9071..9659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9560..9579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9813..9830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9900..9920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9905..10234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10085..10220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
       61 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      121 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      181 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      241 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      301 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      361 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      421 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      481 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      541 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      601 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
      661 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
      721 gagtctatag gcccaccccc ttggcttctt atgcgacgga tcgatcccgt aataagcttc
      781 gaggtccgcg gccggccgcg ttgacgcgca cggcaagagg cgaggggcgg ccgctggtga
      841 gagatgggtg cgagagcgtc ggtattaagc gggggagaat tagataaatg ggaaaaaatt
      901 cggttaaggc cagggggaaa gaaacaatat aaactaaaac atatagtatg ggcaagcagg
      961 gagctagaac gattcgcagt taatcctggc cttttagaga catcagaagg ctgtagacaa
     1021 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1081 aatacaatag cagtcctcta ttgtgtgcat caaaggatag atgtaaaaga caccaaggaa
     1141 gccttagata agatagagga agagcaaaac aaaagtaaga aaaaggcaca gcaagcagca
     1201 gctgacacag gaaacaacag ccaggtcagc caaaattacc ctatagtgca gaacctccag
     1261 gggcaaatgg tacatcaggc catatcacct agaactttaa atgcatgggt aaaagtagta
     1321 gaagagaagg ctttcagccc agaagtaata cccatgtttt cagcattatc agaaggagcc
     1381 accccacaag atttaaatac catgctaaac acagtggggg gacatcaagc agccatgcaa
     1441 atgttaaaag agaccatcaa tgaggaagct gcagaatggg atagattgca tccagtgcat
     1501 gcagggccta ttgcaccagg ccagatgaga gaaccaaggg gaagtgacat agcaggaact
     1561 actagtaccc ttcaggaaca aataggatgg atgacacata atccacctat cccagtagga
     1621 gaaatctata aaagatggat aatcctggga ttaaataaaa tagtaagaat gtatagccct
     1681 accagcattc tggacataag acaaggacca aaggaaccct ttagagacta tgtagaccga
     1741 ttctataaaa ctctaagagc cgagcaagct tcacaagagg taaaaaattg gatgacagaa
     1801 accttgttgg tccaaaatgc gaacccagat tgtaagacta ttttaaaagc attgggacca
     1861 ggagcgacac tagaagaaat gatgacagca tgtcagggag tggggggacc cggccataaa
     1921 gcaagagttt tggctgaagc aatgagccaa gtaacaaatc cagctaccat aatgatacag
     1981 aaaggcaatt ttaggaacca aagaaagact gttaagtgtt tcaattgtgg caaagaaggg
     2041 cacatagcca aaaattgcag ggcccctagg aaaaagggct gttggaaatg tggaaaggaa
     2101 ggacaccaaa tgaaagattg tactgagaga caggctaatt ttttagggaa gatctggcct
     2161 tcccacaagg gaaggccagg gaattttctt cagagcagac cagagccaac agccccacca
     2221 gaagagagct tcaggtttgg ggaagagaca acaactccct ctcagaagca ggagccgata
     2281 gacaaggaac tgtatccttt agcttccctc agatcactct ttggcagcga cccctcgtca
     2341 caataaagat aggggggcaa ttaaaggaag ctctattaga tacaggagca gatgatacag
     2401 tattagaaga aatgaatttg ccaggaagat ggaaaccaaa aatgataggg ggaattggag
     2461 gttttatcaa agtaagacag tatgatcaga tactcataga aatctgcgga cataaagcta
     2521 taggtacagt attagtagga cctacacctg tcaacataat tggaagaaat ctgttgactc
     2581 agattggctg cactttaaat tttcccatta gtcctattga gactgtacca gtaaaattaa
     2641 agccaggaat ggatggccca aaagttaaac aatggccatt gacagaagaa aaaataaaag
     2701 cattagtaga aatttgtaca gaaatggaaa aggaaggaaa aatttcaaaa attgggcctg
     2761 aaaatccata caatactcca gtatttgcca taaagaaaaa agacagtact aaatggagaa
     2821 aattagtaga tttcagagaa cttaataaga gaactcaaga tttctgggaa gttcaattag
     2881 gaataccaca tcctgcaggg ttaaaacaga aaaaatcagt aacagtactg gatgtgggcg
     2941 atgcatattt ttcagttccc ttagataaag acttcaggaa gtatactgca tttaccatac
     3001 ctagtataaa caatgagaca ccagggatta gatatcagta caatgtgctt ccacagggat
     3061 ggaaaggatc accagcaata ttccagtgta gcatgacaaa aatcttagag ccttttagaa
     3121 aacaaaatcc agacatagtc atctatcaat acatggatga tttgtatgta ggatctgact
     3181 tagaaatagg gcagcataga acaaaaatag aggaactgag acaacatctg ttgaggtggg
     3241 gatttaccac accagacaaa aaacatcaga aagaacctcc attcctttgg atgggttatg
     3301 aactccatcc tgataaatgg acagtacagc ctatagtgct gccagaaaag gacagctgga
     3361 ctgtcaatga catacagaaa ttagtgggaa aattgaattg ggcaagtcag atttatgcag
     3421 ggattaaagt aaggcaatta tgtaaacttc ttaggggaac caaagcacta acagaagtag
     3481 taccactaac agaagaagca gagctagaac tggcagaaaa cagggagatt ctaaaagaac
     3541 cggtacatgg agtgtattat gacccatcaa aagacttaat agcagaaata cagaagcagg
     3601 ggcaaggcca atggacatat caaatttatc aagagccatt taaaaatctg aaaacaggaa
     3661 agtatgcaag aatgaagggt gcccacacta atgatgtgaa acaattaaca gaggcagtac
     3721 aaaaaatagc cacagaaagc atagtaatat ggggaaagac tcctaaattt aaattaccca
     3781 tacaaaagga aacatgggaa gcatggtgga cagagtattg gcaagccacc tggattcctg
     3841 agtgggagtt tgtcaatacc cctcccttag tgaagttatg gtaccagtta gagaaagaac
     3901 ccataatagg agcagaaact ttctatgtag atggggcagc caatagggaa actaaattag
     3961 gaaaagcagg atatgtaact gacagaggaa gacaaaaagt tgtcccccta acggacacaa
     4021 caaatcagaa gactgagtta caagcaattc atctagcttt gcaggattcg ggattagaag
     4081 taaacatagt gacagactca caatatgcat tgggaatcat tcaagcacaa ccagataaga
     4141 gtgaatcaga gttagtcagt caaataatag agcagttaat aaaaaaggaa aaagtctacc
     4201 tggcatgggt accagcacac aaaggaattg gaggaaatga acaagtagat aaattggtca
     4261 gtgctggaat caggaaagta ctatttttag atggaataga taaggcccaa gaagaacatg
     4321 agaaatatca cagtaattgg agagcaatgg ctagtgattt taacctacca cctgtagtag
     4381 caaaagaaat agtagccagc tgtgataaat gtcagctaaa aggggaagcc atgcatggac
     4441 aagtagactg tagcccagga atatggcagc tagattgtac acatttagaa ggaaaagtta
     4501 tcttggtagc agttcatgta gccagtggat atatagaagc agaagtaatt ccagcagaga
     4561 cagggcaaga aacagcatac ttcctcttaa aattagcagg aagatggcca gtaaaaacag
     4621 tacatacaga caatggcagc aatttcacca gtactacagt taaggccgcc tgttggtggg
     4681 cggggatcaa gcaggaattt ggcattccct acaatcccca aagtcaagga gtaatagaat
     4741 ctatgaataa agaattaaag aaaattatag gacaggtaag agatcaggct gaacatctta
     4801 agacagcagt acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     4861 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4921 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4981 cagtttggaa aggaccagca aagctcctct ggaaaggtga aggggcagta gtaatacaag
     5041 ataatagtga cataaaagta gtgccaagaa gaaaagcaaa gatcatcagg gattatggaa
     5101 aacagatggc aggtgatgat tgtgtggcaa gtagacagga tgaggattaa cacatggaat
     5161 tctgcaacaa ctgctgttta tccatttcag aattgggtgt cgacatagca gaataggcgt
     5221 tactcgacag aggagagcaa gaaatggagc cagtagatcc tagactagag ccctggaagc
     5281 atccaggaag tcagcctaaa actgcttgta ccaattgcta ttgtaaaaag tgttgctttc
     5341 attgccaagt ttgtttcatg acaaaagcct taggcatctc ctatggcagg aagaagcgga
     5401 gacagcgacg aagagctcat cagaacagtc agactcatca agcttctcta tcaaagcagt
     5461 aagtagtaca tgtaatgcaa cctataatag tagcaatagt agcattagta gtagcaataa
     5521 taatagcaat agttgtgtgg tccatagtaa tcatagaata taggaaaatg gccgctgatc
     5581 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     5641 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     5701 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     5761 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     5821 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     5881 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     5941 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     6001 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6061 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6121 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6181 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     6241 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     6301 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     6361 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     6421 ttagtgaacg gatccttggc acttatctgg gacgatctgc ggagcctgtg cctcttcagc
     6481 taccaccgct tgagagactt actcttgatt gtaacgagga ttgtggaact tctgggacgc
     6541 agggggtggg aagccctcaa atattggtgg aatctcctac aatattggag tcaggagcta
     6601 aagaatagtg ctgttagctt gctcaatgcc acagccatag cagtagctga ggggacagat
     6661 agggttatag aagtagtaca aggagcttgt agagctattc gccacatacc tagaagaata
     6721 agacagggct tggaaaggat tttgctataa gctcgaggcc gccccggtga ccttcagacc
     6781 ttggcactgg aggtggcccg gcagaagcgc ggcatcgtgg atcagtgctg caccagcatc
     6841 tgctctctct accaactgga gaactactgc aactaggccc accactaccc tgtccacccc
     6901 tctgcaatga ataaaacctt tgaaagagca ctacaagttg tgtgtacatg cgtgcatgtg
     6961 catatgtggt gcggggggaa catgagtggg gctggctgga gtggcgatga taagctgtca
     7021 aacatgagaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7081 aatgtcatga taataatggt ttcttagtct agaattaatt ccgtgtattc tatagtgtca
     7141 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     7201 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     7261 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     7321 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     7381 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     7441 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     7501 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     7561 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     7621 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     7681 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7741 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7801 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7861 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7921 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7981 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8041 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8101 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8161 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8221 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8281 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8341 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8401 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8461 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8521 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8581 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8641 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8701 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8761 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8821 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8881 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8941 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9001 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9061 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9181 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9661 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9721 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9781 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9841 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     9901 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9961 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10081 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10141 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10201 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    10261 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    10321 cggactcatg tgaaatactg gtttttagtg cgccagatc
//
