LOCUS       sequence-255529-         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tagless S7-S83C, Delta-157-178 mutant 30S ribosomal 
            protein in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
  TITLE     Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA 
            Folding.
  JOURNAL   Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 
            10.1016/j.cell.2019.10.035. Epub 2019 Nov 21.
  PUBMED    31761533
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.035"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1357-1369.e16"
COMMENT     Sequence Label: pRSF-1b-S7-S83C/Delta-157-178
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(526..544)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gaataatttt gtttaacttt aataaggaga tataccatgc cacgtcgtcg cgtcattggt
       61 cagcgtaaaa ttctgccgga tccgaagttc ggatcagaac tgctggctaa atttgtaaat
      121 atcctgatgg tagatggtaa aaaatctact gctgaatcta tcgtatacag cgcgctggag
      181 accctggctc agcgctctgg taaatctgaa ctggaagcat tcgaagtagc tctcgaaaac
      241 gtgcgcccga ctgtagaagt taagtctcgc cgcgttggtg gttgtactta tcaggtacca
      301 gttgaagtcc gtccggttcg tcgtaatgct ctggcaatgc gttggatcgt tgaagctgct
      361 cgaaaacgcg gtgataaatc catggctctg cgcctggcga acgaactttc tgatgctgca
      421 gaaaacaaag gtactgcagt taagaaacgt gaagacgttc accgtatggc cgaagccaac
      481 aaggcgttcg cacactaccg ttggtgatta acctaggtgc tgccaccgct gagcaataac
      541 tagcataacc ccttggggcc tctaaacggg tcttg
//
