LOCUS       sequence-255533-         447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
  TITLE     Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA 
            Folding.
  JOURNAL   Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 
            10.1016/j.cell.2019.10.035. Epub 2019 Nov 21.
  PUBMED    31761533
REFERENCE   2  (bases 1 to 447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.035"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1357-1369.e16"
COMMENT     Sequence Label: pET-S9
FEATURES             Location/Qualifiers
     source          1..447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatatacat atgggcagca gccatcatca tcatcatcac tggggcgctg aaaatcaata
       61 ctacggcact ggtcgccgca aaagttccgc agctcgcgtt ttcatcaaac cgggcaacgg
      121 taaaatcgta atcaaccaac gttctctgga acagtacttc ggtcgtgaaa ctgcccgcat
      181 ggtagttcgt cagccgctgt gtctggtcga catggttgag aaactggacc tgtacatcac
      241 cgttaaaggt ggtggtatct ctggtcaggc tggtgcgatc cgtcacggta tcacccgcgc
      301 tctgatggaa tacgacgagt ccctgcgttc tgaactgcgt aaagctggct tcgttactcg
      361 tgacgctcgt caggttgaac gtaagaaagt cggtctgcgt aaagcacgtc gtcgtccgca
      421 gttctccaaa cgttaaagac aagctta
//
