LOCUS       sequence-25554-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-1
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(142..162)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(142..160)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(181..197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(181..197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(194..216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tgccatgaat tcagagattg atgtgcaaga tcttctcatc ttttttaatt ctttgttagg
       61 cctggtacct ctagagatat cctgcagtgg ccaatgcatt ggttcgcggc cgccaccgcg
      121 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
      181 gtcatagctg tttcctgtgt gaaattgtta tccgctcggg ccctgccata gcctcaggtt
      241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
      301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
      361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      541 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      841 gcggcagggt cggaacagga gagcgcacga nggnagcttc cagggggnaa acgcctggna
      901 tctttatagn cctgncgggg ttcnccacct ctgacttgac cccgattttt tgngangcnc
      961 nncagggggg cgaacctatg naaaanncnn caacgcggcc tttttanggn nccggncntt
     1021 tgnnggcntt tgcccnnang tnnttncngn nt
//
