LOCUS       sequence-255540-        4152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: SUN2delSUNec
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             852..875
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             879..905
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1741..1840)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1857..1876)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1858..1876)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1881..1897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1881..1897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1999..2018)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2010..2819
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2077..2096)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2966..3554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3455..3474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3708..3725
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3884..3911)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4003..4089)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4138..4152,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4152,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg cttcatggtg tccaagggcg aggaggacaa catggccatc attaaggagt
      181 tcatgcgctt caaggtccac atggagggct ccgttaatgg gcatgagttc gagatcgagg
      241 gggagggcga ggggaggccc tacgagggca cacagactgc caagctgaag gtgacgaagg
      301 gcgggcctct ccccttcgcc tgggacatcc tgtcgcctca gttcatgtac ggctctaagg
      361 cctacgtgaa gcaccccgct gacattccag attacctcaa gctgagcttc ccggagggct
      421 tcaagtggga gcgggttatg aacttcgagg acggcggggt ggtcacggtg acccaggact
      481 ccagcctcca ggatggcgag ttcatctaca aggtcaagct gcgcggcaca aatttccctt
      541 ccgacgggcc ggttatgcag aagaagacta tgggctggga ggcctcgtct gagcggatgt
      601 acccggagga tggcgcgctc aagggggaga ttaagcagcg cctcaagctg aaggacggtg
      661 gccactacga tgctgaggtg aagaccacgt acaaggccaa gaagccggtc cagctcccag
      721 gcgcctacaa cgttaatatc aagctggaca ttacatcgca caacgaggat tacactatcg
      781 tggagcagta cgagagggcc gagggcaggc attctacggg cgggatggac gagctgtaca
      841 agggcgccgg ggattacaag gacgatgacg ataaggcgta cccttacgac gtccccgatt
      901 acgcgggcgc cgggatggct tctccctcat tcgcggccgc tgctacctct ccggcgtcat
      961 ccccactcac gctggacgct atcccactgg cctcaaggcc cgccccaggc gccgctgcgg
     1021 cccagcggaa gcgctcggtg ttcctcctga accaccggcc tcatccggtc tcgcccaccc
     1081 cgcccctgca gccaaccgct gcggcctccg ctcagcacgc caggaggaag aagacctctc
     1141 atccacctag gcccaggtgg cagaccgtgc tctccatcgc tgccaagaat gccgctctcc
     1201 tggcggccct cctgtacctc ggcgacctgg cctggaggtg gagccacccg cccccaccta
     1261 gcccgccccc agaccgggct gccctggagg gctacgcggc tcgcgtcgat gaggttgagg
     1321 ccagcctcac acggactttc cgcatgatcc aggtccagct ggaggcggtt gacaggaaga
     1381 ttgatggcga agtgggggcg gcgcggggcg acctcctggc gctcctggag gataagaggc
     1441 tggctctgga gaggcagctc acgcgcctgg acgctagggc cggcgagctg ggggatgcgc
     1501 tcgctggcct gaagaggatg gagttcctcc ggaaggacga gttcgagaag ttctgggatg
     1561 aggtgaaggg ctcactgggc tcctcctccg agtcggaggt ggacctcgat caggtccggg
     1621 ccctggcgcg cgagatcgtc atgcgcgaga ttgagaagca cgctgcggac ggcatcgggc
     1681 gggtggatta cgctgtggct tccgtcagcc ccggcagctc ggctgggtcg caggcctgag
     1741 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     1801 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1861 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1921 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1981 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     2041 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2101 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     2161 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     2221 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     2281 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     2341 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2401 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2461 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2521 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2581 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2641 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2701 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2761 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2821 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     2881 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3661 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3721 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3781 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3841 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3901 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3961 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4021 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4081 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     4141 agtcacgacg tt
//
