LOCUS       sequence-255541-        4566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW
  TITLE     Identification and characterization of genes encoding the nuclear 
            envelope LINC complex in the monocot species Zea mays.
  JOURNAL   J Cell Sci. 2019 Feb 11;132(3). pii: jcs.221390. doi: 
            10.1242/jcs.221390.
  PUBMED    30659121
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-02-11"; volume: "132(3). pii: jcs.221390. doi"; 
            pages: " 10.1242/jcs.221390"
COMMENT     Sequence Label: SUN2delCCec
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             852..875
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             879..905
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2155..2254)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2271..2290)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2272..2290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2295..2311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2295..2311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2413..2432)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2424..3233
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2491..2510)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3380..3968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3869..3888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4122..4139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4298..4325)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4417..4503)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4552..4566,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4566,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg cttcatggtg tccaagggcg aggaggacaa catggccatc attaaggagt
      181 tcatgcgctt caaggtccac atggagggct ccgttaatgg gcatgagttc gagatcgagg
      241 gggagggcga ggggaggccc tacgagggca cacagactgc caagctgaag gtgacgaagg
      301 gcgggcctct ccccttcgcc tgggacatcc tgtcgcctca gttcatgtac ggctctaagg
      361 cctacgtgaa gcaccccgct gacattccag attacctcaa gctgagcttc ccggagggct
      421 tcaagtggga gcgggttatg aacttcgagg acggcggggt ggtcacggtg acccaggact
      481 ccagcctcca ggatggcgag ttcatctaca aggtcaagct gcgcggcaca aatttccctt
      541 ccgacgggcc ggttatgcag aagaagacta tgggctggga ggcctcgtct gagcggatgt
      601 acccggagga tggcgcgctc aagggggaga ttaagcagcg cctcaagctg aaggacggtg
      661 gccactacga tgctgaggtg aagaccacgt acaaggccaa gaagccggtc cagctcccag
      721 gcgcctacaa cgttaatatc aagctggaca ttacatcgca caacgaggat tacactatcg
      781 tggagcagta cgagagggcc gagggcaggc attctacggg cgggatggac gagctgtaca
      841 agggcgccgg ggattacaag gacgatgacg ataaggcgta cccttacgac gtccccgatt
      901 acgcgggcgc cgggatggct tctccctcat tcgcggccgc tgctacctct ccggcgtcat
      961 ccccactcac gctggacgct atcccactgg cctcaaggcc cgccccaggc gccgctgcgg
     1021 cccagcggaa gcgctcggtg ttcctcctga accaccggcc tcatccggtc tcgcccaccc
     1081 cgcccctgca gccaaccgct gcggcctccg ctcagcacgc caggaggaag aagacctctc
     1141 atccacctag gcccaggtgg cagaccgtgc tctccatcgc tgccaagaat gccgctctcc
     1201 tggcggccct cctgtacctc ggcgacctgg cctggaggtg gagccacccg cccccaccta
     1261 gcccgccccc agaccgggct gccctggagg gctacgcggc tcgcgtcgat gaggttgagg
     1321 ccagcctcac acggactttc cgcatgatcc aggtccagct ggaggcggtt gacaggaaga
     1381 ttgatggcga agtgggggcg gcgcggggcg gggatgcgct cgctggcctg aagaggatgg
     1441 agttcctccg gaaggacgag ttcgagaagt tctgggatga ggtgaagggc tcactgggct
     1501 cctcctccga gtcggaggtg gacctcgatc aggtccgggc cctggcgcgc gagatcgtca
     1561 tgcgcgagat tgagaagcac gctgcggacg gcatcgggcg ggtggattac gctgtggctt
     1621 ccggcggggg cagggttgtg cggcatagcg aggcctacgt tcccaagagg ggcttcatgg
     1681 tgtggatgtc cggcgtggac gttgggccca agccagagaa gatgctgcag cctagcttcg
     1741 gcgagcccgg gcagtgcttc gctctccagg gctcaaacgg gttcgtggag gtcaagctga
     1801 agtccggcat cattccagag gcggtgaccc tggagcacgt ttctaaggac gtggcctacg
     1861 atcgctcaac ggcgcctaag ggctgcaggg tctacgggtg gtacgaggag acgcccggcg
     1921 agacccagtc ggggcatgcc gctaagatgg cgctcgctga gttcgcctac gacctggaga
     1981 agaacaatgt tcagacattc gacgtgactg cgccggatgt tggcgtgatc aacatggtgc
     2041 gcctcgactt cacatcgaat cacggctcat cccagctcac ttgcatctac cgcctgcggg
     2101 tgcacggcca tgagcctgtc agccccggca gctcggctgg gtcgcaggcc tgagacccag
     2161 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     2221 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2281 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2341 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2401 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     2461 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2521 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2581 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2641 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2701 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2761 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2821 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2881 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2941 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     3001 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     3061 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     3121 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3181 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     3241 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     3301 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4141 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4201 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4261 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4321 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4381 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4441 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4501 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     4561 gacgtt
//
