LOCUS       sequence-255544-        9905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 
            3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2707..2772
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2782..3370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2835..2855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3253..3264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3384..3883
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3419..3442)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3816..3835
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3904..4503
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3904..3923)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4400..4420
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4641..4874
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4946..5067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4983..5002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5037..5056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5107..5242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5262..5281)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5263..5281)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5291..5308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5291..5307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5300..5322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5449..5904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5536..5555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5746..5767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5930..6034
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6035..6103,6104..6895)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6253..6272)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7066..7654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7555..7574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7808..7825
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7942..7963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7978..7983,7984..8001,8002..8008)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8016..8032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8021..8043
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8040..8056
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8040..8056
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8075..8095
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8077..8095
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8123..8349
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8350..8530
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8577..8702
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9195..9428
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9613..9657
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatgatgaa
     1981 gatgattgtt tcatacttga gaaagcagct agagggaaaa ggcctatttt tgaatgtttt
     2041 tggaatggac gattaatacc atatacatca gttgaagact ttgactggtg tactcctcct
     2101 aagaagagag ggcttgcacc aattgaatgc tacaatagga tatctggtgc attattcact
     2161 aatgacaaat tccaggtcag cacaaataaa ttgacgttta tggatcttga gctaaaattg
     2221 aaagataaga acaccctttt tacaaggatt ttaaatggac aggaacagcg aatgaaaatt
     2281 gacagagaat ttgctttgtg gctgaaggac tgtcatgaga agtatgataa acaaataaaa
     2341 tttacacttt ttaagggagt aattacacgt cctgatcttc cttctaaaaa gcaaggtccc
     2401 tgggcaacat atgcagcaat agaatgggat ggaaagatat acaaagcagg acagctggtc
     2461 aagacaatca agacacttcc cctcttttat ggaagcatag taagattttt tctttatggc
     2521 gatcatgatg gagaagtata tgctacagga ggagaggttc aaattgcaat gatgactccc
     2581 atacgtaagt gtaatgactc attgcgtcat tcaccaaagg ttgagacgac agattgtcca
     2641 gttcctccta aaagaatgag acgagaagct acaagacaaa ataggattat aaccaaaaca
     2701 gatgtagact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     2761 gacgatgaca agtgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3181 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3361 gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     3421 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     3481 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     3541 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     3601 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     3661 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     3721 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     3781 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     3841 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     3901 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     3961 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     4021 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     4081 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     4141 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     4201 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     4261 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     4321 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     4381 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     4441 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     4501 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct
     4561 cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4621 tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct
     4681 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4741 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4801 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4861 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     4921 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     4981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5041 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     5101 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5161 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5221 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     5281 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     5341 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     5401 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     5461 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5521 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5581 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5641 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5701 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5761 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5821 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5881 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5941 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6001 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6061 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6121 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6181 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6241 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6301 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6361 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6421 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6481 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6541 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6601 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6661 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6721 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6781 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6841 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6901 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6961 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7141 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7201 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7261 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7321 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7381 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7441 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7501 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7561 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7621 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7681 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7741 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7801 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7861 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7921 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7981 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8041 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     8101 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     8161 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     8221 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     8281 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     8341 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8401 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8461 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8521 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     8581 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8641 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8701 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8761 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8821 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8881 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8941 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9001 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     9061 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9121 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9181 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9241 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9301 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9361 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9421 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9481 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     9541 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     9601 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     9661 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     9721 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     9781 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     9841 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     9901 ggatc
//
