LOCUS       sequence-255546-        6161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ETF1 with an E55D substitution.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Katrekar D, Moreno AM, Chen G, Worlikar A, Mali P
  TITLE     Oligonucleotide conjugated multi-functional adeno-associated 
            viruses.
  JOURNAL   Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.1038/s41598-018-21742-x.
  PUBMED    29483550
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-02-26"; volume: "8(1):3589. doi"; pages: " 
            10.1038/s41598-018-21742-x"
COMMENT     Sequence Label: pCAG-ETF1-E55D
FEATURES             Location/Qualifiers
     source          1..6161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          157..1173
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1097..1119
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1181..1200
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1266..1275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2678..2697)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2743..2798
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2797..2816)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3159..3175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3159..3175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3172..3194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3183..3199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3207..3213,3214..3231,3232..3237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3252..3273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3332..3527
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3378..3513
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3533..3667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3570..3589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3624..3643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3735..3752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3906..4494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3986..4005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4665..5456,5457..5525))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5288..5307
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5526..5630)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5661..6040
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(6042..6161,1..156)
                     /label=chicken beta-actin promoter
ORIGIN
        1 sgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
       61 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      121 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      181 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      241 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      301 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      361 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      421 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      481 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
      541 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
      601 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
      661 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
      721 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
      781 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
      841 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
      901 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
      961 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1021 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1081 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1141 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1201 ctgtctcatc attttggcaa agaattctgc agtcgacggt accgcgggcc cgggatccac
     1261 cggtcgccac catggcggac gaccccagtg ctgccgacag gaacgtggag atctggaaga
     1321 tcaagaagct cattaagagc ttggaggcgg cccgcggcaa tggcaccagc atgatatcat
     1381 tgatcattcc tcccaaagac cagatttcac gagtggcaaa aatgttagcg gatgactttg
     1441 gaactgcatc taacattaag tcacgagtaa accgcctttc agtcctggga gccattacat
     1501 ctgtacaaca aagactcaaa ctttataaca aagtacctcc aaatggtctg gttgtatact
     1561 gtggaacaat tgtaacagaa gaaggaaagg aaaagaaagt caacattgac tttgaacctt
     1621 tcaaaccaat taatacgtca ttgtatttgt gtgacaacaa attccataca gaggctctta
     1681 cagcactact ttcagatgat agcaagtttg gattcattgt aatagatggt agtggtgcac
     1741 tttttggcac actccaagga aacacaagag aagtcctgca caaattcact gtggatctcc
     1801 caaagaaaca cggtagagga ggtcagtcag ccttgcgttt tgcccgttta agaatggaaa
     1861 agcgacataa ctatgttcgg aaagtagcag agactgctgt gcagctgttt atttctgggg
     1921 acaaagtgaa tgtggctggt ctagttttag ctggatccgc tgactttaaa actgaactaa
     1981 gtcaatctga tatgtttgat cagaggttac aatcaaaagt tttaaaatta gttgatatat
     2041 cctatggtgg tgaaaatgga ttcaaccaag ctattgagtt atctactgaa gtcctctcca
     2101 acgtgaaatt cattcaagag aagaaattaa taggacgata ctttgatgaa atcagccagg
     2161 acacgggcaa gtactgtttt ggcgttgaag atacactaaa ggctttggaa atgggagctg
     2221 tagaaattct aatagtctat gaaaatctgg atataatgag atatgttctt cattgccaag
     2281 gcacagaaga ggagaaaatt ctctatctaa ctccagagca agaaaaggat aaatctcatt
     2341 tcacagacaa agagaccgga caggaacatg agcttatcga gagcatgccc ctgttggaat
     2401 ggtttgctaa caactataaa aaatttggag ctacgttgga aattgtcaca gataaatcac
     2461 aagaagggtc tcagtttgtg aaaggatttg gtggaattgg aggtatcttg cggtaccgag
     2521 tagatttcca gggaatggaa taccaaggag gagacgatga attttttgac cttgatgact
     2581 actaatgata gagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     2641 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     2701 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     2761 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     2821 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     2881 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     2941 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3001 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3061 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     3121 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     3181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3241 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3301 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     3361 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3421 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3481 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     3541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3601 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3661 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3721 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3781 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3841 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4321 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4501 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4561 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4621 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4681 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4741 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4801 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4861 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4921 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4981 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5041 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5101 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5161 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5221 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5281 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5341 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5401 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5461 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5641 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     5701 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5761 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5821 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5881 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     5941 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     6001 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     6061 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     6121 ttaattattt tgtgcagcga tgggggcggg gggggggggg g
//
