LOCUS       sequence-255548-        7327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the AAV2 capsid with the N587 residue replaces with a TAG 
            stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Katrekar D, Moreno AM, Chen G, Worlikar A, Mali P
  TITLE     Oligonucleotide conjugated multi-functional adeno-associated 
            viruses.
  JOURNAL   Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.1038/s41598-018-21742-x.
  PUBMED    29483550
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-02-26"; volume: "8(1):3589. doi"; pages: " 
            10.1038/s41598-018-21742-x"
COMMENT     Sequence Label: pAAV2-N587X
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..48
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3997..4008
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4483..4500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4483..4499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4492..4514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4640..5095)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4778..4799)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4989..5008
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5122..5226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5227..5295,5296..6087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5445..5464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6258..6846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6747..6766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7000..7017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7134..7155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7170..7175,7176..7193,7194..7200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7208..7224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7213..7235
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7232..7248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7232..7248
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7298..7314
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7298..7314
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttctg atctgcgcag
       61 ccgccatgcc ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc
      121 tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc
      181 cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc
      241 tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag gcccttttct
      301 ttgtgcaatt tgagaaggga gagagctact tccacatgca cgtgctcgtg gaaaccaccg
      361 gggtgaaatc catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa ctgattcaga
      421 gaatttaccg cgggatcgag ccgactttgc caaactggtt cgcggtcaca aagaccagaa
      481 atggcgccgg aggcgggaac aaggtggtgg atgagtgcta catccccaat tacttgctcc
      541 ccaaaaccca gcctgagctc cagtgggcgt ggactaatat ggaacagtat ttaagcgcct
      601 gtttgaatct cacggagcgt aaacggttgg tggcgcagca tctgacgcac gtgtcgcaga
      661 cgcaggagca gaacaaagag aatcagaatc ccaattctga tgcgccggtg atcagatcaa
      721 aaacttcagc caggtacatg gagctggtcg ggtggctcgt ggacaagggg attacctcgg
      781 agaagcagtg gatccaggag gaccaggcct catacatctc cttcaatgcg gcctccaact
      841 cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa
      901 ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt
      961 ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat
     1021 gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg
     1081 ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact
     1141 ggaccaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatc tggtgggagg
     1201 aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg
     1261 tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca
     1321 cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc
     1381 agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg
     1441 ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg
     1501 aggtggagca tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg
     1561 acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag
     1621 acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa caaatgttct cgtcacgtgg
     1681 gcatgaatct gatgctgttt ccctgcagac aatgcgagag aatgaatcag aattcaaata
     1741 tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     1801 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa
     1861 aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct
     1921 ttgaacaata aatgatttaa atcaggtatg gctgccgatg gttatcttcc agattggctc
     1981 gaggacactc tctctgaagg aataagacag tggtggaagc tcaaacctgg cccaccacca
     2041 ccaaagcccg cagagcggca taaggacgac agcaggggtc ttgtgcttcc tgggtacaag
     2101 tacctcggac ccttcaacgg actcgacaag ggagagccgg tcaacgaggc agacgccgcg
     2161 gccctcgagc acgacaaagc ctacgaccgg cagctcgaca gcggagacaa cccgtacctc
     2221 aagtacaacc acgccgacgc ggagtttcag gagcgcctta aagaagatac gtcttttggg
     2281 ggcaacctcg gacgagcagt cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg
     2341 gttgaggaac ctgttaagac ggctccggga aaaaagaggc cggtagagca ctctcctgtg
     2401 gagccagact cctcctcggg aaccggaaag gcgggccagc agcctgcaag aaaaagattg
     2461 aattttggtc agactggaga cgcagactca gtacctgacc cccagcctct cggacagcca
     2521 ccagcagccc cctctggtct gggaactaat acgatggcta caggcagtgg cgcaccaatg
     2581 gcagacaata acgagggcgc cgacggagtg ggtaattcct cgggaaattg gcattgcgat
     2641 tccacatgga tgggcgacag agtcatcacc accagcaccc gaacctgggc cctgcccacc
     2701 tacaacaacc acctctacaa acaaatttcc agccaatcag gagcctcgaa cgacaatcac
     2761 tactttggct acagcacccc ttgggggtat tttgacttca acagattcca ctgccacttt
     2821 tcaccacgtg actggcaaag actcatcaac aacaactggg gattccgacc caagagactc
     2881 aacttcaagc tctttaacat tcaagtcaaa gaggtcacgc agaatgacgg tacgacgacg
     2941 attgccaata accttaccag cacggttcag gtgtttactg actcggagta ccagctcccg
     3001 tacgtcctcg gctcggcgca tcaaggatgc ctcccgccgt tcccagcaga cgtcttcatg
     3061 gtgccacagt atggatacct caccctgaac aacgggagtc aggcagtagg acgctcttca
     3121 ttttactgcc tggagtactt tccttctcag atgctgcgta ccggaaacaa ctttaccttc
     3181 agctacactt ttgaggacgt tcctttccac agcagctacg ctcacagcca gagtctggac
     3241 cgtctcatga atcctctcat cgaccagtac ctgtattact tgagcagaac aaacactcca
     3301 agtggaacca ccacgcagtc aaggcttcag ttttctcagg ccggagcgag tgacattcgg
     3361 gaccagtcta ggaactggct tcctggaccc tgttaccgcc agcagcgagt atcaaagaca
     3421 tctgcggata acaacaacag tgaatactcg tggactggag ctaccaagta ccacctcaat
     3481 ggcagagact ctctggtgaa tccgggcccg gccatggcaa gccacaagga cgatgaagaa
     3541 aagttttttc ctcagagcgg ggttctcatc tttgggaagc aaggctcaga gaaaacaaat
     3601 gtggacattg aaaaggtcat gattacagac gaagaggaaa tcaggacaac caatcccgtg
     3661 gctacggagc agtatggttc tgtatctacc aacctccaga gaggctagag acaagcagct
     3721 accgcagatg tcaacacaca aggcgttctt ccaggcatgg tctggcagga cagagatgtg
     3781 taccttcagg ggcccatctg ggcaaagatt ccacacacgg acggacattt tcacccctct
     3841 cccctcatgg gtggattcgg acttaaacac cctcctccac agattctcat caagaacacc
     3901 ccggtacctg cgaatccttc gaccaccttc agtgcggcaa agtttgcttc cttcatcaca
     3961 cagtactcca cgggacaggt cagcgtggag atcgagtggg agctgcagaa ggaaaacagc
     4021 aaacgctgga atcccgaaat tcagtacact tccaactaca acaagtctgt taatgtggac
     4081 tttactgtgg acactaatgg cgtgtattca gagcctcgcc ccattggcac cagatacctg
     4141 actcgtaatc tgtaattgct tgttaatcaa taaaccgttt aattcgtttc agttgaactt
     4201 tggtctctgc gtatttcttt cttatctagt ttccatggct acgtagataa gtagcatggc
     4261 gggttaatca ttaactacag cccgggcgtt taaacagcgg gcggaggggt ggagtcgtga
     4321 cgtgaattac gtcatagggt tagggaggtc ctgtattaga ggtcacgtga gtgttttgcg
     4381 acattttgcg acaccatgtg gtctcgctgg gggggggggc ccgagtgagc acgcagggtc
     4441 tccattttga agcgggaggt ttgaacgagc gctggcgcgc tcactggccg tcgttttaca
     4501 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4561 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4621 cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4681 ttttgttaaa tcagctcatt tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4741 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4801 attaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4861 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4921 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4981 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     5041 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     5101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7021 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7081 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7141 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7201 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7261 aagcgcgccg atatcgttaa cgccccgcgc cggccgctct agaactagtg gatcccccgg
     7321 aagatca
//
