LOCUS       sequence-25555-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-2
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(112..315)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(141..161)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        316..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccccggggan gctctcgccc ttgctcacca tggtggcgac cggtggatcc cggagcttga
       61 gctcgagatc tgagtccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct
      121 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      181 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      241 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      301 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
      361 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
      421 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
      481 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
      541 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
      601 taccgtaagt tatgtaacgc ggaactccat atatggtann nnacnanggc cccccgtant
      661 tgattactat aaatanctaa tgctgcgtat acgtntccnc accggganng ggggnaannc
      721 gcagaaaaan tgtgtgacca aaggccacaa angccagnan ccnaaaangg cccgttgctg
      781 gnnttttcnn aggtccnccc cctganaa
//
