LOCUS       sequence-255552-       10037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2839..2904
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2914..3502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2967..2987)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3385..3396)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3516..4015
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3551..3574)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3948..3967
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4036..4635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4036..4055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4532..4552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4773..5006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5078..5199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5115..5134)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5169..5188
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5239..5374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5394..5413)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5395..5413)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5423..5440)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5423..5439)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5432..5454)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5581..6036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5668..5687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5878..5899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6062..6166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6167..6235,6236..7027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6385..6404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7198..7786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7687..7706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7940..7957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8074..8095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8110..8115,8116..8133,8134..8140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8148..8164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8153..8175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8172..8188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8172..8188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8207..8227
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8209..8227
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8255..8481
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8482..8662
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8709..8834
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9327..9560
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9745..9789
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgatagtttt
     1981 gaattcaaag ctcatgttga aggagatggt gtagtggaag ggattatccg ttatcatcca
     2041 ttcttatatg atagagaaac ttaccctgat gatccatgct ttccatcaaa attaaaagat
     2101 gaagatgatg aagatgattg tttcatactt gagaaagcag ctagagggaa aaggcctatt
     2161 tttgaatgtt tttggaatgg acgattaata ccatatacat cagttgaaga ctttgactgg
     2221 tgtactcctc ctaagaagag agggcttgca ccaattgaat gctacaatag gatatctggt
     2281 gcattattca ctaatgacaa attccaggtc agcacaaata aattgacgtt tatggatctt
     2341 gagctaaaat tgaaagataa gaacaccctt tttacaagga ttttaaatgg acaggaacag
     2401 cgaatgaaaa ttgacagaga atttgctttg tggctgaagg actgtcatga gaagtatgat
     2461 aaacaaataa aatttacact ttttaaggga gtaattacac gtcctgatct tccttctaaa
     2521 aagcaaggtc cctgggcaac atatgcagca atagaatggg atggaaagat atacaaagca
     2581 ggacagctgg tcaagacaat caagacactt cccctctttt atggaagcat agtaagattt
     2641 tttctttatg gcgatcatga tggagaagta tatgctacag gaggagaggt tcaaattgca
     2701 atgatgactc ccatacgtaa gtgtaatgac tcattgcgtc attcaccaaa ggttgagacg
     2761 acagattgtc cagttcctcc taaaagaatg agacgagaag ctacaagaca aaataggatt
     2821 ataaccaaaa cagatgtaga ctacaaagac catgacggtg attataaaga tcatgacatc
     2881 gattacaagg atgacgatga caagtgagtc gacaatcaac ctctggatta caaaatttgt
     2941 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3001 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3061 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3121 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3181 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3241 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3301 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     3361 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3421 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3481 tccctttggg ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag
     3541 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     3601 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     3661 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     3721 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     3781 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     3841 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     3901 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     3961 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     4021 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4081 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4141 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4201 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4261 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4321 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4381 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4441 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4501 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4561 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4621 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     4681 ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     4741 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     4801 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4861 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4921 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4981 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5041 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5101 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5161 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5221 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5281 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5341 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5401 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5461 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5521 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     5581 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5641 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5701 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5761 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5821 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5881 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5941 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6001 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6061 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6181 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6241 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6301 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6361 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6421 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6481 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6541 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6601 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6661 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6721 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6781 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6841 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6901 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6961 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7021 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7081 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7141 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7321 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7861 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7921 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7981 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8041 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8101 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8161 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8221 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8281 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8341 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     8401 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     8461 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     8521 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8581 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8641 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     8701 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8761 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8821 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8881 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     8941 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9001 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9061 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9121 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9181 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9241 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9301 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9361 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9421 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9481 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9541 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9601 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     9661 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9721 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9781 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9841 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9901 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     9961 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
    10021 cgattagtga acggatc
//
