LOCUS       sequence-25556-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-2
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(189..205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(189..205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(202..224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      403..994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggggangct tgngaatttc agagaatctc gtcaaagcta gttcttgctt ttttaattct
       61 ttgttaggcc tggtacctct agagatatcc tgcagtggcc aatgcattgg ttcgcggccg
      121 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
      181 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcgggcc ctgccatagc
      241 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      301 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      361 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      421 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      481 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      541 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      601 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      661 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      721 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      781 cctacagcgt gagctatgag aaaagcgcca cgcttcccga agggagaaag gcggacaggt
      841 atccggtaag cggcagggtc ggaacaggag agcgcacgan gganccttcc agggggaana
      901 cgcctgggna tctttatagt cctgtcgggt ttcgccacct ctgactganc ncccattttt
      961 gtgatgcncg tcaggggggc ggagcctatg gaaaaangcc agcaacccgg cctttttacg
     1021 gttccnggcc tttggctggn cctttggccc nangttcntt tccngngta
//
