LOCUS       sequence-255566-       12633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant nuclear envelope LINC protein fused to 
            fluorescent protein and affinity tags for cell biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Gumber HK, McKenna JF, Tolmie AF, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     MLKS2 is an ARM domain and F-actin-associated KASH protein that 
            functions in stomatal complex development and meiotic chromosome 
            segregation.
  JOURNAL   Nucleus. 2019 Dec;10(1):144-166. doi: 10.1080/19491034.2019.1629795.
  PUBMED    31221013
REFERENCE   2  (bases 1 to 12633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "144-166"
COMMENT     Sequence Label: MLKS2exp
FEATURES             Location/Qualifiers
     source          1..12633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3176..3200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3572..3751
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3787..4812
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      5010..5262
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5355..5379
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5900..6691
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6937..7525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7426..7445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7679..7696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7711..7851
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7777..7796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7937..7959
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8195..8389
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8455..9528)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9957..10586)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11917..11941
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggggtggtc cccatcctgg tggagctgga tggcgacgtt
      541 aacgggcaca agttctcggt gtctggcgag ggggagggcg acgctaccta cgggaagctc
      601 acgctgaagt tcatctgcac gaccggcaag ctgccggtgc cctggccaac actcgtcaca
      661 actctgactt acggcgtcca gtgcttctcg cgctaccctg atcacatgaa gcagcatgac
      721 ttcttcaagt cggctatgcc cgagggctac gtgcaggagc ggacgatctt cttcaaggac
      781 gatggcaact acaagacacg cgcggaggtc aagttcgagg gggacactct cgttaacagg
      841 atcgagctga agggcattga cttcaaggag gatggcaata tcctcgggca caagctggag
      901 tacaactaca attcccataa cgtgtacatc atggccgaca agcagaagaa cggcattaag
      961 gtcaatttca agatccggca caacattgag gatggcagcg ttcagctcgc ggaccattac
     1021 cagcagaaca caccgatcgg ggatggccct gtcctcctgc ccgacaatca ctacctctcc
     1081 actcagagcg ccctgtcgaa ggacccgaac gagaagaggg accatatggt gctcctggag
     1141 ttcgtcacag ccgcgggcat cactctcggg atggatgagc tgtacaaggg ggcgggcgac
     1201 tacaaggacg atgacgataa ggcttacccc tacgacgtgc cggattacgc cggggctggc
     1261 atggggcgct cactctcccc gctcctgagg caggagctgg acaacctgga taaggacgcc
     1321 gattctcgca gggctgccat gaaggctctc aagtcatacg cccgccacct ggatagcaag
     1381 tcgatccccc atttcctggc cgaggtctct gacacgaccg ccgctggcgc gggggccggc
     1441 agcgggatgc caaccggcgg ggagttcacg atctcgctct acgaggtcct ggccagggtt
     1501 cacgggcgga acatcgtgcc ccagatcggc aatattatgg cgaccattat gtgcacgctc
     1561 tccagctcgg gcgggtcatt cccactgcac caggcctgct ccaaggttgt gccagctatc
     1621 gctaggcatg gcattgatcc gtcggcccca gacggcgaga aggccgggat cattgctagc
     1681 ctctgccgcc cactgtgctc ggctctcatg ggcgggagcc aggacggcgg cgctgccagc
     1741 ggcgctgccc tctgcctgaa ggccctggtt gagtcttcaa actggaggtt cgcgtcgggc
     1801 gagctggtga atgaggtctg cctgaaggtg gccggcgcta tgcacgactc ggccacacgg
     1861 tcgaacgctc atatggggct ggccatggcg ctcgtgaagc acaatggcct catcgctgag
     1921 gcctacgcgc gctctatcgt caggtccggc ctccagattc tggacgggga taccgctgac
     1981 tccagctcgc agaagaggct gtccgccatc cagatgatta acttcttcat gaagttcgtg
     2041 gacccgaggt gcctgtcttc agagctgggc cgggttatcg atgtgatgga gcgctgccag
     2101 aatgaccgga tgcccttcgt gcggggcgcg gctttcgagg cctctcagtc agcgcgcaac
     2161 attgccgcgc agaagggctc caggcacgag gttgggacct ctcctatggt gggctcaaac
     2221 ttccataaga ggagggagaa gtccccttgc cggagcctct ggggcgccaa gaggtccccc
     2281 gctgcctcta cgatggccgc tgctagccct gtgcagttcc gctctccgga gtcacaggtc
     2341 gttgattcca gcatcatgaa tggctccaca ctccacgact ccccagtgag cgtcggccag
     2401 tcgtcttgca acttcgacca gagcaggcgg actaaccgca ggctgtggtc gaatgacgcg
     2461 gttgatgtgt ctctgaagga cggcctcttc atccagctgt gctccaacag caattcgtac
     2521 gaggacgatc tgggcgaggt ttgcgatagc gaggtgaccg acgctaactt cgagtgcacc
     2581 aatacgttcg ccgggttcgt ctctccctca cacaatggct ccatcagcag ggataagacg
     2641 ccttcaccgc gggcctcgga caggcccatc aacattgacg atgtgaagat ctactccacg
     2701 ccaaggaagc tcctgcggct cctgcagtca tcctacgact cggattctgc ctcaaacatc
     2761 gggcagagca cggccaagct caggggcctc tcctcccccg accaggagca caagggcctc
     2821 gtcatttctt cagagcagat gcagtcgctg cattctgaca ataagggcga tgagatgaag
     2881 gacgagaacg agaccatcta catgcagaat agcaagggca ggacacagac tctctcgaac
     2941 gctgacgcct cagtcctgtc cacaactgag gccgagaaca caagcatcaa gacttcgcct
     3001 gagattgagc tgaatgggga cgatgtttgc atcacctcca gcattggcaa gacgcggaag
     3061 taccgcgcga agttcaccct cttcctgtcc gtcatcgtta ttttcctcgc tatcattacg
     3121 atgctgatcc gcattgagaa cgacgatgac agcgcgtacc tggtgccgac ctgagaccca
     3181 gctttcttgt acaaagtggt gatatcccgc ggccatgcta gagtccgcaa aaatcaccag
     3241 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     3301 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     3361 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     3421 aaaatccagt gacctgcagg catgcgacgt cgggccctct agaggatccc cgggtaccgc
     3481 gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     3541 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactgagc
     3601 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     3661 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     3721 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aactcgaatc
     3781 ccccctatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     3841 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     3901 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     3961 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     4021 gacattgggg agttcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     4081 acgttgcaag acctgcctga aaccgaactg cccgctgttc ttcagccggt cgcggaggct
     4141 atggatgcta tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     4201 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     4261 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     4321 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     4381 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     4441 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     4501 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     4561 tcgccaaggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     4621 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     4681 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     4741 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     4801 gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg cctcagccag
     4861 caactcgcgc gagcctagca aggcaaatgc gagagaacgg ccttacgctt ggtggcacag
     4921 ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc gggagggccg gtcgcagtga
     4981 ttcaggaatt aattccctag agtcaagcag atcgttcaaa catttggcaa taaagtttct
     5041 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     5101 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     5161 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     5221 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgcaagctga
     5281 taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     5341 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5401 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5461 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5521 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     5581 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     5641 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     5701 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5761 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5821 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5881 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5941 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     6001 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     6061 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     6121 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     6181 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     6241 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     6301 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     6361 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     6421 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     6481 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     6541 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6601 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6661 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6721 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     6781 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     6841 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7081 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7621 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7681 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     7741 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7801 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7861 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7921 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7981 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     8041 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     8101 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     8161 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     8221 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     8281 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     8341 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     8401 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     8461 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     8521 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     8581 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8641 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     8701 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     8761 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     8821 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     8881 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8941 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     9001 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     9061 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     9121 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     9181 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     9241 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     9301 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     9361 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     9421 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     9481 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     9541 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     9601 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     9661 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9721 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9781 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9841 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9901 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9961 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    10021 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    10081 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    10141 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    10201 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    10261 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    10321 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    10381 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    10441 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    10501 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    10561 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    10621 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    10681 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10741 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10801 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10861 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10921 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10981 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    11041 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    11101 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    11161 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    11221 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    11281 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    11341 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    11401 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    11461 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    11521 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    11581 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    11641 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    11701 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11761 gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatccc gaggggaacc
    11821 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    11881 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
    11941 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctaag
    12001 cttgagctct cccatatggt cgactagagc caagctgatc tcctttgccc cggagatcac
    12061 catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag aaggtgacga
    12121 taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa agaatgctga
    12181 cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct acccgagtaa
    12241 taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag
    12301 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc
    12361 agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc
    12421 taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga tcgaggatct
    12481 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
    12541 caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact ccaagaatat
    12601 caaagataca gtctcagaag accaaagggc tat
//
