LOCUS       sequence-25557-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-3
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(110..313)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(139..159)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        314..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcccggnang ctctcgccct tgctcccatg gtggcgaccg gtggatcccg gagcttgagc
       61 tcgagatctg agtccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta
      121 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      181 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      241 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
      301 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
      361 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
      421 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
      481 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
      541 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
      601 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac ccctaaattg
      661 attactatta ataactaatg catggcggta atacggttat ccacagatcg ggaaccagaa
      721 naaaaaanat ttntgancaa ncggccacna angnccagaa ccgtaaaaag ccccgtngct
      781 ggcgtttttc cataggctcc nccccctgac ancatcaaaa at
//
