LOCUS       sequence-25558-e        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-3
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(144..164)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(144..162)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(183..199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(183..199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(196..218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gatcgggctc ttttcagaga atagcaccga atctgttgaa acttttttaa ttctttgtta
       61 ggcctggtac ctctagagat atcctgcagt ggccaatgca ttggttcgcg gccgccaccg
      121 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
      181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctcg ggccctgcca tagcctcagg
      241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
      301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      841 aagcggcagg gtcggaacag gagancgcac gangggagct tccaggggga ancgccnggn
      901 atctttatag tcctgtcggg gtttcgccac ctctgacttg ancgccgant ttttgtgang
      961 ccncnncagg gggcggancc nanggaaaaa acnccagcaa cncggccttt ttacggtncc
     1021 gggcntttgc nggcctttng cncnnaggtn cttnccgnnn ta
//
