LOCUS       sequence-25561-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-5
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(109..312)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(138..158)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        313..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cccgggacgc tctcgccctt gctcccatgg tggcgaccgg tggatcccgg agcttgagct
       61 cgagatctga gtccggtagc gctagcggat ctgacggttc actaaaccag ctctgcttat
      121 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      181 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      241 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      301 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      361 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      421 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      481 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
      541 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
      601 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgnac ccgtaattga
      661 ttactattaa taactaatgc atggcngtan tacgntnccc aatcnggnag ggnaaaacgc
      721 agaaaaaacn tgtgagcaan ggccagcaaa ngncaggacc gtaaaaagcc ccgttgctgg
      781 cgttttcata gnctcncccc ctgacaacat caaaaat
//
