LOCUS       sequence-25562-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-5
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(145..165)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(145..163)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(184..200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(184..200)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(197..219)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     gap             839..849
                     /estimated_length=11
ORIGIN
        1 gncggnnctg ttcttcagag agaacagtta ccggagcatc atctttttta attctttgtt
       61 aggcctggta cctctagaga tatcctgcag tggccaatgc attggttcgc ggccgccacc
      121 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      181 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc gggccctgcc atagcctcag
      241 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      781 agcgtgagct atgagaaagc gccacgcttc cgaaggagaa gcgaagncgg angagcnann
      841 nnnnnnnnna ancggcaggg tcngnaacag gagagcgcac gangggagct tccaggggga
      901 aaccggtgnn natctttata gtccctgtcg ggtttcgcca cctctgaact ggagcgtcga
      961 ntttttgnga tgctcgtcag gggggncgga gcctatggaa aaancgccag caacgcggcc
     1021 ntttttacgg ntccngggcc ttttggctgg ccttttgncn ccaanggttc ntttcccggc
     1081 gtancccccc gaatnctggg gnaaancng
//
