LOCUS       sequence-255628-        7710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Beraud C, Sun SC, Ganchi P, Ballard DW, Greene WC
  TITLE     Human T-cell leukemia virus type I Tax associates with and is 
            negatively regulated by the NF-kappa B2 p100 gene product: 
            implications for viral latency.
  JOURNAL   Mol Cell Biol. 1994 Feb . 14(2):1374-82.
  PUBMED    8289813
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1994 Feb . 14(2):1374-82."
COMMENT     Sequence Label: pCMV4-p100
FEATURES             Location/Qualifiers
     source          1..7710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3537..3553)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3537..3553)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    3561..4183
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3760..3779)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4207..4227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4212..4541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4392..4527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4580..4676
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(4752..4771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4753..4771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4785..4801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4785..4801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4798..4820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4809..4825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4833..4839,4840..4857,4858..4863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4878..4899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5016..5033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5187..5775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5267..5286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5946..6737,6738..6806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6569..6588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6807..6911)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6938..7393
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7025..7044)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7235..7256
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7519..7541
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7533..7550
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7534..7550
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgttt ttatttttaa ttttctttca aatacttcca tcgaattcag atctggtacc
      661 acgcgtatcg ataagcttca ccatggagag ttgctacaac ccaggtctgg atggtattat
      721 tgaatatgat gatttcaaat tgaactcctc cattgtggaa cccaaggagc cagccccaga
      781 aacagctgat ggcccctacc tggtgatcgt ggaacagcct aagcagagag gcttccgatt
      841 tcgatatggc tgtgaaggcc cctcccatgg aggactgccc ggtgcctcca gtgagaaggg
      901 ccgaaagacc tatcccactg tcaagatctg taactacgag ggaccagcca agatcgaggt
      961 ggacctggta acacacagtg acccacctcg tgctcatgcc cacagtctgg tgggcaagca
     1021 atgctcggag ctggggatct gcgccgtttc tgtggggccc aaggacatga ctgcccaatt
     1081 taacaacctg ggtgtcctgc atgtgactaa gaagaacatg atggggacta tgatacaaaa
     1141 acttcagagg cagcggctcc gctctaggcc ccagggcctt acggaggccg agcagcggga
     1201 gctggagcaa gaggccaaag aactgaagaa ggtgatggat ctgagtatag tgcggctgcg
     1261 cttctctgcc ttccttagag ccagtgatgg ctccttctcc ctgcccctga agccagtcat
     1321 ctcccagccc atccatgaca gcaaatctcc gggggcatca aacctgaaga tttctcgaat
     1381 ggacaagaca gcaggctctg tgcggggtgg agatgaagtt tatctgcttt gtgacaaggt
     1441 gcagaaagat gacattgagg ttcggttcta tgaggatgat gagaatggat ggcaggcctt
     1501 tggggacttc tctcccacag atgtgcataa acagtatgcc attgtgttcc ggacaccccc
     1561 ctatcacaag atgaagattg agcggcctgt aacagtgttt ctgcaactga aacgcaagcg
     1621 aggaggggac gtgtctgatt ccaaacagtt cacctattac cctctggtgg aagacaagga
     1681 agaggtgcag cggaagcgga ggaaggcctt gcccaccttc tcccagccct tcgggggtgg
     1741 ctcccacatg ggtggaggct ctgggggtgc agccgggggc tacggaggag ctggaggagg
     1801 tggcagcctc ggtttcttcc cctcctccct ggcctacagc ccctaccagt ccggcgcggg
     1861 ccccatgggc tgctacccgg gaggcggggg cggggcgcag atggccgcca cggtgcccag
     1921 cagggactcc ggggaggaag ccgcggagcc gagcgccccc tccaggaccc cccagtgcga
     1981 gccgcaggcc ccggagatgc tgcagcgagc tcgagagtac aacgcgcgcc tgttcggcct
     2041 ggcgcagcgc agcgcccgag ccctactcga ctacggcgtc accgcggacg cgcgcgcgct
     2101 gctggcggga cagcgccacc tgctgacggc gcaggacgag aacggagaca caccactgca
     2161 cctagccatc atccacgggc agaccagtgt cattgagcag atagtctatg tcatccacca
     2221 cgcccaggac ctcggcgttg tcaacctcac caaccacctg caccagacgc ccctgcacct
     2281 ggcggtgatc acggggcaga cgagtgtggt gagctttctg ctgcgggtag gtgcagaccc
     2341 agctctgctg gatcggcatg gagactcagc catgcatctg gcgctgcggg caggcgctgg
     2401 tgctcctgag ctgctgcgtg cactgcttca gagtggagct cctgctgtgc cccagctgtt
     2461 gcatatgcct gactttgagg gactgtatcc agtacacctg gcggtccgag cccgaagccc
     2521 tgagtgcctg gatctgctgg tggacagtgg ggctgaagtg gaggccacag agcggcaggg
     2581 gggacgaaca gccttgcatc tagccacaga gatggaggag ctggggttgg tcacccatct
     2641 ggtcaccaag ctccgggcca acgtgaacgc tcgcaccttt gcgggaaaca cacccctgca
     2701 cctggcagct ggactggggt acccgaccct cacccgcctc cttctgaagg ctggtgctga
     2761 catccatgct gaaaacgagg agcccctgtg cccactgcct tcacccccta cctctgatag
     2821 cgactcggac tctgaagggc ctgagaagga cacccgaagc agcttccggg gccacacgcc
     2881 tcttgacctc acttgcagca ccaaggtgaa gaccttgctg ctaaatgctg ctcagaacac
     2941 catggagcca cccctgaccc cgcccagccc agcagggccg ggactgtcac ttggtgatac
     3001 agctctgcag aacctggagc agctgctaga cgggccagaa gcccagggca gctgggcaga
     3061 gctggcagag cgtctggggc tgcgcagcct ggtagacacg taccgacaga caacctcacc
     3121 cagtggcagc ctcctgcgca gctacgagct ggctggcggg gacctggcag gtctactgga
     3181 ggccctgtct gacatgggcc tagaggaggg agtgaggctg ctgaggggtc cagaaacccg
     3241 agacaagctg cccagcacag aggtgaagga agacagtgcg tacgggagcc agtcagtgga
     3301 gcaggaggca gagaagctgg gcccaccccc tgagccacca ggagggctct gccacgggca
     3361 cccccagcct caggtgcact gacctgctgc ctgcccccag cccccttccc ggaccccctg
     3421 tacagcgtcc ccacctattt caaatcttat ttaacacccc acacccaccc ctcagttggg
     3481 acaaataaag gattctcatg ggaaggggag gaccccggaa ttcctgcagc ccgggggatc
     3541 cactagttct agaggatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     3601 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     3661 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     3721 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3781 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3841 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3901 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3961 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     4021 ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac
     4081 agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg
     4141 tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagctt
     4201 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4261 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4321 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4381 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4441 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4501 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc gaggaactga
     4561 aaaaccagaa agttaactgg taagtttagt ctttttgtct tttatttcag gtcccggatc
     4621 cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac ttctaggcct
     4681 gtacggaagt gttacttctg ctctaaaagc tgctgcaggt cgactctagg gccgcgtacc
     4741 gagctcgaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct
     4801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4921 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4981 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     6961 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7021 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7081 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7141 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7201 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7261 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7321 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7381 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7441 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7501 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga
     7561 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7621 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7681 ttggctcatg tccaatatga ccgccatgtt
//
