LOCUS       sequence-25563-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-6
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(114..317)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(143..163)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        318..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gncnccgggg acgctctcgc ccttgctcac catggtggcg accggtggat cccggagctt
       61 gagctcgaga tctgagtccg gtagcgctag cggatctgac ggttcactaa accagctctg
      121 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      181 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      241 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      301 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      361 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
      421 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
      481 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
      541 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
      601 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
      661 attgattact attaataana aatgcagggc ggtaatacng tacnacggaa atncgggaaa
      721 caccgcaaga aaaaatnntg ngancaaagg ccagcaaagg ccaggaccgt aaaggcgcgt
      781 gctgggnttt catagctcgc ccctgacaac nc
//
