LOCUS       sequence-25564-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-6
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(147..167)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(147..165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(186..202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(186..202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(199..221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gnggggtacg ngcattcaga gatttcgtcg ataacacacc agatcttttt taattctttg
       61 ttaggcctgg tacctctaga gatatcctgc agtggccaat gcattggttc gcggccgcca
      121 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcgggccctg ccatagcctc
      241 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      301 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      361 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      421 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      481 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      541 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      601 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      661 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      721 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      781 acagcgggga gctatganaa agcgccncgc ttccgangga gancnaagga gaaaggnggg
      841 aaagggatan ccggtnaaag cggcanggtc ggaancngga aaacncccca ggggagcttn
      901 cnggggggaa aaccccnggg aatctttata nnncnnnngg nngggntgtc ncccnccnct
      961 gantcngagc ggcnganttt ttnngaagnc ncccncnggg gggggggggn gncccntngg
     1021 naaaaanncc ccccaacccc ggcnccnttt tnnagggggn cncgggggnn tttttggggg
     1081 gncttt
//
