LOCUS       sequence-255668-       11088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant nuclear envelope LINC protein fused to 
            fluorescent protein and affinity tags for cell biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Gumber HK, McKenna JF, Tolmie AF, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     MLKS2 is an ARM domain and F-actin-associated KASH protein that 
            functions in stomatal complex development and meiotic chromosome 
            segregation.
  JOURNAL   Nucleus. 2019 Dec;10(1):144-166. doi: 10.1080/19491034.2019.1629795.
  PUBMED    31221013
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "144-166"
COMMENT     Sequence Label: MLKS2delARMexp
FEATURES             Location/Qualifiers
     source          1..11088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1631..1655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2027..2206
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2242..3267
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3465..3717
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3810..3834
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4355..5146
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5392..5980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5881..5900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6134..6151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6166..6306
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6232..6251)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6392..6414
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6650..6844
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6910..7983)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8412..9041)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10372..10396
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggggtggtc cccatcctgg tggagctgga tggcgacgtt
      541 aacgggcaca agttctcggt gtctggcgag ggggagggcg acgctaccta cgggaagctc
      601 acgctgaagt tcatctgcac gaccggcaag ctgccggtgc cctggccaac actcgtcaca
      661 actctgactt acggcgtcca gtgcttctcg cgctaccctg atcacatgaa gcagcatgac
      721 ttcttcaagt cggctatgcc cgagggctac gtgcaggagc ggacgatctt cttcaaggac
      781 gatggcaact acaagacacg cgcggaggtc aagttcgagg gggacactct cgttaacagg
      841 atcgagctga agggcattga cttcaaggag gatggcaata tcctcgggca caagctggag
      901 tacaactaca attcccataa cgtgtacatc atggccgaca agcagaagaa cggcattaag
      961 gtcaatttca agatccggca caacattgag gatggcagcg ttcagctcgc ggaccattac
     1021 cagcagaaca caccgatcgg ggatggccct gtcctcctgc ccgacaatca ctacctctcc
     1081 actcagagcg ccctgtcgaa ggacccgaac gagaagaggg accatatggt gctcctggag
     1141 ttcgtcacag ccgcgggcat cactctcggg atggatgagc tgtacaaggg ggcgggcgac
     1201 tacaaggacg atgacgataa ggcttacccc tacgacgtgc cggattacgc cggggctggc
     1261 atggggaagg gcctcgtcat ttcttcagag cagatgcagt cgctgcattc tgacaataag
     1321 ggcgatgaga tgaaggacga gaacgagacc atctacatgc agaatagcaa gggcaggaca
     1381 cagactctct cgaacgctga cgcctcagtc ctgtccacaa ctgaggccga gaacacaagc
     1441 atcaagactt cgcctgagat tgagctgaat ggggacgatg tttgcatcac ctccagcatt
     1501 ggcaagacgc ggaagtaccg cgcgaagttc accctcttcc tgtccgtcat cgttattttc
     1561 ctcgctatca ttacgatgct gatccgcatt gagaacgacg atgacagcgc gtacctggtg
     1621 ccgacctgag acccagcttt cttgtacaaa gtggtgatat cccgcggcca tgctagagtc
     1681 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     1741 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     1801 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     1861 ctaattccta aaaccaaaat ccagtgacct gcaggcatgc gacgtcgggc cctctagagg
     1921 atccccgggt accgcgaatt atcgatcatg agcggagaat taagggagtc acgttatgac
     1981 ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg
     2041 aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac
     2101 cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca
     2161 aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca
     2221 ctctcaactc gaatcccccc tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     2281 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     2341 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     2401 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     2461 attccggaag tgcttgacat tggggagttc agcgagagcc tgacctattg catctcccgc
     2521 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttcttcag
     2581 ccggtcgcgg aggctatgga tgctatcgct gcggccgatc ttagccagac gagcgggttc
     2641 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     2701 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     2761 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     2821 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     2881 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     2941 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     3001 ccggagcttg caggatcgcc aaggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     3061 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     3121 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     3181 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     3241 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga
     3301 gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta
     3361 cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag
     3421 ggccggtcgc agtgattcag gaattaattc cctagagtca agcagatcgt tcaaacattt
     3481 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     3541 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     3601 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     3661 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgac
     3721 cggcatgcaa gctgataatt caattcggcg ttaattcagt acattaaaaa cgtccgcaat
     3781 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     3841 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     3901 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     3961 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     4021 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc
     4081 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4141 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4201 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4261 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4321 gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt
     4381 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4441 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4501 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4561 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4621 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4681 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     4741 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     4801 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     4861 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     4921 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     4981 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     5041 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     5101 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa
     5161 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc
     5221 acataattgc tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc
     5281 ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga
     5341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5521 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     6181 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     6241 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     6301 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6361 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6421 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
     6481 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     6541 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     6601 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     6661 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     6721 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     6781 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     6841 cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     6901 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     6961 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     7021 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     7081 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     7141 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     7201 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     7261 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     7321 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     7381 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     7441 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     7501 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     7561 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     7621 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     7681 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     7741 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
     7801 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     7861 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     7921 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     7981 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     8041 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     8101 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     8161 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     8221 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     8281 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     8341 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     8401 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     8461 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     8521 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     8581 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     8641 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     8701 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     8761 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     8821 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     8881 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     8941 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     9001 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     9061 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     9121 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     9181 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
     9241 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
     9301 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
     9361 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
     9421 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
     9481 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
     9541 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
     9601 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
     9661 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
     9721 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
     9781 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
     9841 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
     9901 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
     9961 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
    10021 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
    10081 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
    10141 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
    10201 gcagcggagg ggttggatca aagtacttta aagtacttta aagtacttta aagtactttg
    10261 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    10321 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
    10381 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
    10441 caagctcaag ctaagcttga gctctcccat atggtcgact agagccaagc tgatctcctt
    10501 tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga
    10561 cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt
    10621 cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc tcatcaagac
    10681 gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
    10741 agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat
    10801 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
    10861 agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat
    10921 tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct
    10981 tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt
    11041 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctat
//
