LOCUS       sequence-255669-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged late endosome marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(857..874)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gcgggagggg cgcgggactc tcggcatgga cgagctgtac aagtactcag atctcgacat
       61 gacctctagg aagaaagtgt tgctgaaggt tatcatcctg ggagattctg gagtcgggaa
      121 gacatcactc atgaaccagt atgtgaataa gaaattcagc aatcagtaca aagccacaat
      181 aggagctgac tttctgacca aggaggtgat ggtggatgac aggctagtca caatgcagat
      241 atgggacaca gcaggacagg aacggttcca gtctctcggt gtggccttct acagaggtgc
      301 agactgctgc gttctggtat ttgatgtgac tgcccccaac acattcaaaa ccctagatag
      361 ctggagagat gagtttctca tccaggccag tccccgagat cctgaaaact tcccatttgt
      421 tgtgttggga aacaagattg acctcgaaaa cagacaagtg gccacaaagc gggcacaggc
      481 ctggtgctac agcaaaaaca acattcccta ctttgagacc agtgccaagg aggccatcaa
      541 cgtggagcag gcgttccaga cgattgcacg gaatgcactt aagcaggaaa cggaggtgga
      601 gctgtacaac gaatttcctg aacctatcaa actggacaag aatgaccggg ccaaggcctc
      661 ggcagaaagc tgcagttgct gaggatcccg cccctctccc tccccccccc ctaacgttac
      721 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
      781 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
      841 tcctagggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
      901 gcagttcctc tggaagcttc ttgagacaac aacgtctgta gcgacccttt gcaggcagcg
      961 aaccccccac ctggcgacag gtgcctctgc ggccaaagcc acgtgtataa gaacacctgc
     1021 aagcgcacac ccagtgccgt gtgagtgata gtgtgaagag tcaatgctct ctcagcgtat
     1081 cacaggctga gatgccagta ccatgtatgg aatcgatggg ctcgccatgc ctatggctag
     1141 tcagtaagac gtagccgacc aggactggaa ttccattca
//
