LOCUS       sequence-255670-       12375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12375)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 12375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: MKAKU41exp
FEATURES             Location/Qualifiers
     source          1..12375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1189..1212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1216..1242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2918..2942)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3314..3493
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3529..4554
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4752..5004
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5097..5121
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5642..6433
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6679..7267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7168..7187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7421..7438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7453..7593
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7519..7538)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7679..7701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7937..8131
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8197..9270)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9699..10328)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11659..11683
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggttagc
      481 aagggagaag aggacaatat ggcgataatc aaagagttca tgcggttcaa ggttcacatg
      541 gagggctcgg tcaacgggca cgaattcgag attgaaggag agggcgaagg aaggccatac
      601 gagggcactc agacagcaaa gttgaaggtc accaagggcg gaccactccc tttcgcttgg
      661 gacattctgt ctccacagtt catgtacggc tccaaggcct atgtgaagca tccagcagac
      721 atccctgatt atctcaagct gagcttccct gaaggcttca agtgggagag agttatgaat
      781 tttgaagatg gtggcgtggt taccgtcact caggactctt cactgcaaga tggagagttc
      841 atctacaagg tgaagcttcg cggtactaac tttccatctg acggccctgt tatgcagaag
      901 aagacaatgg gttgggaagc ctccagcgag aggatgtatc ctgaagatgg tgcactgaag
      961 ggcgagatca agcagagact taagttgaag gacggaggtc actacgatgc tgaggtcaag
     1021 actacatata aggccaagaa gccagtgcaa ttgcctggcg cttacaatgt taacattaag
     1081 ctcgacatca cttcccacaa tgaggattac acaatcgttg agcagtatga aagggccgag
     1141 ggaagacata gcactggcgg catggatgag ttgtacaagg gtgcaggcga ttataaggat
     1201 gacgatgaca aggcttaccc atatgacgtg cctgattacg caggagctgg tatggcaagc
     1261 ctcttcggag ctaggagacg caggtcacca gaatatgatg gtgaagatga tagatccggt
     1321 ggcggaaggg caaagagaag aaggctgagc cctgaggaag ctgccgcatc tccagcagag
     1381 cctggagctg ccacaggtac cagccatggc tggcttagcg gatttgtctc tggtgctaag
     1441 agggccattt cttcagtgct cctgtccagc tctccagagg aaactggctc tggagaagac
     1501 ggagaggtcg aggaagagga tgacgatgtg tacgaagagg gcatcgattt gaatgagaac
     1561 gaagacattc acgatatcca tggtgaaatt gtcccttatt ctgagtcaaa gctcgccatc
     1621 gaacaaatgg ttatgaagga gacattctca agagacgaat gcgatcgcat ggttgagctg
     1681 attaagtcaa gagtccgcga ttccactcca gaaacacacg agtacggcaa gcaggaagag
     1741 attccttcca gaaatgcagg aatcgctcat gacttcactg gtacatgccg ctcccttagc
     1801 agggatagaa acttcacaga gtctgtgcca ttttcatcca tgcgcatgag gcctggccac
     1861 agctctccag gatttcctct tcaggcttca ccacaattgt gcaccgcagc tgtgagggaa
     1921 gccaagaagt ggcttgaaga gaagagacaa ggtttgggcg ttaagccaga ggacaatggc
     1981 tcttgcaccc tcaacactga tattttctca tcccgcgacg attcagacaa gggttcccct
     2041 gttgatcttg ccaagagcta tatgaggtct ttgccacctt ggcagtcccc attcctcgga
     2101 catcaaaagt ttgacacctc tccttcaaag tacagcatca gctctactaa ggttaccact
     2161 aaggaggatt atctctcatc cttttggact aagctggaag agtctagaat tgctcgcatc
     2221 ggcagctctg gagactcagc cgtcgcatcc aagctgtgga attacggctc caacagcaga
     2281 cttttcgaaa atgatacctc aatcttttcc ctgggcactg acgagaaggt tggagatcct
     2341 accaagactc acaacggcag cgaaaaggtc gccgcaaccg agccacttgg acgctgctct
     2401 cttttgatta cacctaccga agacaggact gatgtgtcat ataaggttgc cgagggcaat
     2461 gacgtgtcat ccacaggaat caccaaggac acaaccggtc attcagccga tggcaaggca
     2521 ctcacatccg aaccacacat tggcgagact catgttaatt ctgcatcaga gtccatccct
     2581 aacgatgctg ccccacctac tcagtctaag atgaatggta gcacaaagaa gtctctcgtc
     2641 aacggcgtgc tggaccaacc aaatgctaac tcaggattgg agagctctgg taatgattac
     2701 ccttcctata caaactcatc cagcgctatg ccacctgcca gcaccgagct cattggttct
     2761 gcagctgccg ttatcgacgt cgattctgct gaaaacggac caggtactaa gccagagcag
     2821 cctgccaagg gcgcatcaag ggctagcaag tctaaggttg tgccacgggg acagaagagg
     2881 gtgctgcggt cggctacacg gggacgggct acgtgagacc cagctttctt gtacaaagtg
     2941 gtgatatccc gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
     3001 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
     3061 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     3121 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca
     3181 ggcatgcgac gtcgggccct ctagaggatc cccgggtacc gcgaattatc gatcatgagc
     3241 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
     3301 tttggaactg acagaaccgc aacgttgaag gagccactga gccgcgggtt tctggagttt
     3361 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
     3421 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
     3481 cggtatccaa ttagagtctc atattcactc tcaactcgaa tcccccctat gaaaaagcct
     3541 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     3601 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     3661 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     3721 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagttcagc
     3781 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     3841 gaaaccgaac tgcccgctgt tcttcagccg gtcgcggagg ctatggatgc tatcgctgcg
     3901 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     3961 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     4021 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     4081 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     4141 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     4201 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     4261 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccaag gctccgggcg
     4321 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     4381 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     4441 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     4501 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga
     4561 tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc gcgagcctag
     4621 caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc cacagttcgc
     4681 taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggaa ttaattccct
     4741 agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4801 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4861 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4921 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4981 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     5041 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     5101 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     5161 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     5221 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     5281 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     5341 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg
     5401 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     5461 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     5521 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     5581 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     5641 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5701 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5761 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5821 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5881 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5941 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     6001 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     6061 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     6121 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     6181 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     6241 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     6301 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     6361 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     6421 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     6481 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     6541 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc
     6601 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6661 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6721 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6781 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6841 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6901 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6961 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7021 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7081 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7141 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7201 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7261 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7321 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7381 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7441 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7501 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7561 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7621 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7681 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     7741 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     7801 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     7861 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     7921 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     7981 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8041 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     8101 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     8161 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     8221 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     8281 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     8341 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     8401 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     8461 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     8521 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     8581 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     8641 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     8701 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     8761 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     8821 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     8881 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     8941 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9001 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9061 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     9121 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     9181 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     9241 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     9301 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     9361 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     9421 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
     9481 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
     9541 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
     9601 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
     9661 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
     9721 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
     9781 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     9841 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     9901 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     9961 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10021 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10081 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    10141 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    10201 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    10261 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    10321 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    10381 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    10441 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    10501 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    10561 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    10621 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    10681 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    10741 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    10801 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    10861 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    10921 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    10981 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11041 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    11101 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    11161 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    11221 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    11281 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    11341 agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc
    11401 ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac
    11461 tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag tactttaaag
    11521 tactttaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    11581 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
    11641 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
    11701 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagcta agcttgagct ctcccatatg
    11761 gtcgactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
    11821 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
    11881 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
    11941 ggcctacgcg gcaggtctca tcaagacgat ctacccgagt aataatctcc aggagatcaa
    12001 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
    12061 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
    12121 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
    12181 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
    12241 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
    12301 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
    12361 agaccaaagg gctat
//
