LOCUS       sequence-255672-       13644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 13644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: NCH2exp
FEATURES             Location/Qualifiers
     source          1..13644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             3694..3702
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4187..4211)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4583..4762
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4798..5823
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      6021..6273
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6366..6390
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6911..7702
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7948..8536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8437..8456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8690..8707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8722..8862
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8788..8807)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8948..8970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9206..9400
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9466..10539)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10968..11597)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12928..12952
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccaatcctcg tcgagctgga cggggatgtt
      541 aacggccaca agttctccgt gagcggcgag ggggagggcg acgccaccta cgggaagctc
      601 acgctgaagt tcatttgcac gaccggcaag ctgccggtgc cctggccaac cctggtcaca
      661 actctgacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc ggagggctac gttcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagacaag ggcggaggtt aagttcgagg gcgacactct cgtgaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctcggcca caagctggag
      901 tacaactaca attcgcataa cgtctacatc atggctgaca agcagaagaa cggcattaag
      961 gttaatttca agatccgcca caatattgag gacggctccg tgcagctcgc ggatcattac
     1021 cagcagaaca cgccgatcgg ggacggccct gtcctcctgc cggataatca ctacctctcg
     1081 acgcagtctg cgctgtcaaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacag ccgcggggat cactctcggc atggacgagc tgtacaaggg ggcgggcgac
     1201 tacaaggacg atgacgataa ggcttacccc tacgacgtgc cagattacgc tggggccggc
     1261 ggatccatgg cgagcccgcg ctcggccggc ggtgacgagg cgatctggat gaagctgcgc
     1321 gaggccgggt tcgacgagga cgctgtcagg cgccgcgaca aggcggccct catcggctac
     1381 atctcgcggc tcgagtccga gatctatgaa tatcagcaca atcttgggct tattttattg
     1441 gagcggaagg agttgacgtc caaatacgaa caactcaaag cttcttttga agccactgag
     1501 atcatactca agcgagagcg agcttcgcag cagtctgctt tatctgaaac aagaaagagg
     1561 gaagaaaacc taaaaaaaaa tctagccatt cagaaggagt gcatatcaaa tcttgaaaag
     1621 gcacttcatg atatgcgcgg ggaaacagct gaaataaaag tttcatatga agcaaaactg
     1681 gctgaatccc ttcaaatgat ggagacggca cagaaaaagt ttgatgaagc agaagagaaa
     1741 cttctcactg caaaatcatt agaagcagac tgcattcgaa ctcgcaacac ctctctgaga
     1801 agactgcaag acattgaaga tcgcgaagat cagctaagaa gataccaaac atcttttgaa
     1861 cttgagaatg catcgaaaga gaaagagatt aacctcctga ggaaatcgtt ggatgatacc
     1921 aaaaaaattt tgcatgaaaa ggagcaatgt ttactgaaag aacaagtact tcttaatcag
     1981 agggatgaca gcatccttga gagacttgcg tacataacta gctcagagaa aaggttggag
     2041 gaggaaaagt taaatctcga agatgaaaga aaggttcttt tggaagagaa aaataagctg
     2101 gatctgaaca tgcaggcaat tatatccagg gaggaagcta taattcagaa ggaatccata
     2161 cttgacaaac gtgaaagtga actattgatt ctccaagaga ccattgcaag caaagaaagg
     2221 gctgaaatag aaaggctccg tcaagaggaa gaaattgcct tggtgaggag gaggcaggaa
     2281 tttgacactg acatggaaat taagctcact tcttttgagg aagagattga tgcaaggaaa
     2341 gccttgctcg atcaaaggga aaccaccatc aatgagaagg aggatgcagt ggcgcaaagg
     2401 gaacagaatc tcaaccttag gtttgctgag ttagcgaaca aggaagaatc tttggtgaag
     2461 aaatcagatg agttaaggga ggaagagaaa aggctctcct ctgagagaga gacactgcac
     2521 attgatctgc aaaaagaaaa ggaggaaatt cataacatga aattggattt agagaaggaa
     2581 aaatctttct ttgaggagga gaagcgtgaa gcgattcaag ctcaggagaa tctagcaata
     2641 acccaaaatg agcgagaaga tttacaaagt ttgcaggtga aacttaaaga tgaaattgat
     2701 agtctgagag cccaaaaggt tgacctaatg gctgatgcag aaaggctatt atcagaaaaa
     2761 gaaaggtttg agattgaatg ggagttgatc gatgagaaga aggaggaact acaaaaggag
     2821 gcagccagga ttgctgaaga acgaagagta atagatgagc atctcaagag cgaatttgat
     2881 attatcaaac aagaaaagga agatctccgt gttcagttaa aaattagtac agaatccctt
     2941 gctcatgaac atgctgaatt catgaataag atgcagcaag agcatgctag ttggcttagc
     3001 aggatacaat tagaaagaga agatctgaag aaagatattg atatccagag aaccgagtta
     3061 ctgaattctg caaaggctag gcagatggaa atagattcat atttgaggga aaaagaagag
     3121 gagtttgaac agaaaaaatc caaggaactt gaatacatca attctgaaaa ggaaactatc
     3181 agctcaaaac tagaacatgt caggcttgaa ttgcaaaaac ttgaggagga gaggaaaagg
     3241 tctatgctgg agcgagagag aagagaggaa gagctgtctg aaacaaagaa gactatagac
     3301 gcgcttaatg agcaacgcga gaagttacaa gagcaaagaa aactgcttca ttcagaccga
     3361 aaatcaatta cacagcaaat gcagctgctt aatgagttag aagagctgaa gattgaatct
     3421 gagaacaggc aactgtcttt gagacagtgt gggaaatcaa agcatgctgg tgtggaaaac
     3481 ctggaagaca acggtgttca cctgtctcct gatgaggatc aaaatgctag tccaaaacaa
     3541 actactgtaa agaaattgga agtgtcccct tcagtctcca caccaatttc ttgggttaaa
     3601 aaatgtgctc aggtgatatt caaacgctct ccagagaaga gtgctgatcc caacaatgat
     3661 atacctccaa agttgggaaa tgtcaatgac tgtacctcct tagctactgc ctattcagat
     3721 ggattgtttg cttgtcacct ggaaaatggt gctgagaaag taccacaagc tatcgacggt
     3781 ctgaaagttg ggaaaaagag gcttaataat gcactctctc atggtgactc cgaaatttca
     3841 cagccaaaac gcaagcagca aaagagcgct actcagattt tgagagtgat tggaggagaa
     3901 attgattcca actgcccatc agttctagaa gaaaaatgtt caaagaatga acatgatgca
     3961 gtcccagttg gtttgtgtgg gaagggacca caaaatcccc aagcagagca ttcagaggat
     4021 atttcagtgt ctgcatctga accgtcgaac ggacctggtg ttgtggactc tgaggacaag
     4081 gatggagcag atgaggagtc tgacgatgaa ggcgaggacg aggaggaaga gaagacgtca
     4141 tcggcgaaga agctgtggcg tttcctcatc acatgactgc aggcagaccc agctttcttg
     4201 tacaaagtgg tgatatcccg cggccatgct agagtccgca aaaatcacca gtctctctct
     4261 acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg
     4321 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     4381 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     4441 tgacctgcag gcatgcgacg tcgggccctc tagaggatcc ccgggtaccg cgaattatcg
     4501 atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc
     4561 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
     4621 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
     4681 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
     4741 aattcccctc ggtatccaat tagagtctca tattcactct caactcgaat cccccctatg
     4801 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     4861 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     4921 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     4981 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5041 gagttcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     5101 gacctgcctg aaaccgaact gcccgctgtt cttcagccgg tcgcggaggc tatggatgct
     5161 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5221 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5281 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5341 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5401 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5461 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5521 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccaagg
     5581 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5641 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5701 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5761 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     5821 tagagtagat gccgaccgaa caagagctga tttcgagaac gcctcagcca gcaactcgcg
     5881 cgagcctagc aaggcaaatg cgagagaacg gccttacgct tggtggcaca gttctcgtcc
     5941 acagttcgct aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg attcaggaat
     6001 taattcccta gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
     6061 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     6121 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     6181 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     6241 tatcgcgcgc ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat
     6301 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     6361 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     6421 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     6481 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     6541 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     6601 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     6661 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     6721 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     6781 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     6841 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     6901 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     6961 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     7021 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     7081 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     7141 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     7201 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     7261 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     7321 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     7381 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     7441 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     7501 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     7561 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     7621 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     7681 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     7741 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     7801 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     7861 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     8101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8701 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     8761 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8821 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     8881 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8941 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     9001 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     9061 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     9121 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     9181 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     9241 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     9301 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     9361 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     9421 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     9481 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     9541 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     9601 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     9661 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     9721 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     9781 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     9841 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     9901 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     9961 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
    10021 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
    10081 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
    10141 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
    10201 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
    10261 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    10321 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    10381 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    10441 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    10501 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    10561 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
    10621 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    10681 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    10741 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    10801 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    10861 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    10921 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    10981 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    11041 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    11101 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    11161 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    11221 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    11281 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    11341 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    11401 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    11461 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    11521 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    11581 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    11641 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    11701 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    11761 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    11821 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    11881 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    11941 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    12001 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    12061 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    12121 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    12181 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    12241 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    12301 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    12361 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    12421 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    12481 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    12541 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    12601 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    12661 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    12721 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    12781 actttaaagt actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg
    12841 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
    12901 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
    12961 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa gcttgagctc
    13021 tcccatatgg tcgactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga
    13081 ctttctctat ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt
    13141 caccaccgat aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat
    13201 ggttagagag gcctacgcgg caggtctcat caagacgatc tacccgagta ataatctcca
    13261 ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
    13321 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
    13381 gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt
    13441 agttcctact gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact
    13501 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
    13561 aatcttcgtc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
    13621 agtctcagaa gaccaaaggg ctat
//
