LOCUS       sequence-255673-       11779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 11779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: MNEAP1exp
FEATURES             Location/Qualifiers
     source          1..11779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(472..474,475..477,478..1188)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1198..1221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1225..1251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2322..2346)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2718..2897
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2933..3958
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      4156..4408
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4501..4525
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5046..5837
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6083..6671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6572..6591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6825..6842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6857..6997
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6923..6942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7083..7105
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7341..7535
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7601..8674)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9103..9732)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11063..11087
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttgtacaaaa aagcaggctt catggtctcc
      481 aagggggagg agctgttcac cggcgtggtc ccaatcctcg tcgagctgga cggggatgtt
      541 aacggccaca agttctccgt gagcggcgag ggggagggcg acgccaccta cgggaagctc
      601 acgctgaagt tcatttgcac gaccggcaag ctgccggtgc cctggccaac cctggtcaca
      661 actctgacgt acggcgtcca gtgcttctcc cgctacccgg accacatgaa gcagcatgat
      721 ttcttcaaga gcgccatgcc ggagggctac gttcaggagc gcaccatctt cttcaaggac
      781 gatggcaact acaagacaag ggcggaggtt aagttcgagg gcgacactct cgtgaaccgg
      841 atcgagctga agggcattga cttcaaggag gatgggaata ttctcggcca caagctggag
      901 tacaactaca attcgcataa cgtctacatc atggctgaca agcagaagaa cggcattaag
      961 gttaatttca agatccgcca caatattgag gacggctccg tgcagctcgc ggatcattac
     1021 cagcagaaca cgccgatcgg ggacggccct gtcctcctgc cggataatca ctacctctcg
     1081 acgcagtctg cgctgtcaaa ggacccgaac gagaagaggg atcatatggt tctcctggag
     1141 ttcgtgacag ccgcggggat cactctcggc atggacgagc tgtacaaggg ggcgggcgac
     1201 tacaaggacg atgacgataa ggcttacccc tacgacgtgc cagattacgc tggggccggc
     1261 ggatccatgt ctgtctcgga gaaggtggtg ctatcatcat cagtttcttc gtcgtcagat
     1321 ttagatccgt tgctgaaggg tcttacggag aagaagttga gctttaggag gaacgtggtg
     1381 tcattggcag ccgagctcaa ggatgtgagg agcaagcttg cttcccagga gcagctgttt
     1441 gtaagggaat ctcaaactag aaagcttgtg gatacaaaag caaggagcat ggaagaggaa
     1501 gtaagcaagt tgcagaaatg cttacaggac aaggatgagc aactgcgttc atccacaagt
     1561 agcacggaac agtacctcca tgagttggat gatcttagaa cacaactgtc atttactcaa
     1621 gctacagcag aagcaagtgc tgcatcagct aagtcagccc agatgcagtg cttatcattg
     1681 ctgaaagagc tgaatgagaa ggacatctcg ctcaaagagc acgggcttcg agtgaacaag
     1741 ctaggtgagc agcttgatct tctccagagg gaactacaag caagggagct ctcgcaaatg
     1801 caacttaaag atgaagttat aagaatcgag accgacatca tggatgcagt tgccaaagct
     1861 gggtctaggt ctgacaaaga taatgagctt ttaaagatct tatctgatgt ttcaccaagg
     1921 aatgtccaga acctcaacaa ccttttgaat gctaaggata cagaaattgc gaggttgaga
     1981 gaggaaatca gaatattgtc tgctcactgg acgaacaaaa ccaaggaact tgagtcacat
     2041 ttagaaaagc accggagaac tgatcaggag ctgaagaaga gggttctaaa gcttgaattt
     2101 tgccttcaag aatcccagtc tcagatgcga aaattaaaga ggatgggcga gagaagagac
     2161 aaggctctca aggagctcat ggaccaggtg gccacgaagc agccaaacag catgtgccat
     2221 ggcaacaagg agaatttctg ggagggccag ggcttcaagt tcatcgtgtc tatgtcgatg
     2281 ctggcactag tgatcctcgc aaagcgatga cctgcaggca gacccagctt tcttgtacaa
     2341 agtggtgata tcccgcggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa
     2401 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta
     2461 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     2521 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc
     2581 tgcaggcatg cgacgtcggg ccctctagag gatccccggg taccgcgaat tatcgatcat
     2641 gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt
     2701 tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctgagccgcg ggtttctgga
     2761 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
     2821 gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
     2881 ccctcggtat ccaattagag tctcatattc actctcaact cgaatccccc ctatgaaaaa
     2941 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     3001 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     3061 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     3121 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt
     3181 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     3241 gcctgaaacc gaactgcccg ctgttcttca gccggtcgcg gaggctatgg atgctatcgc
     3301 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     3361 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     3421 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     3481 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     3541 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     3601 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     3661 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc caaggctccg
     3721 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     3781 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     3841 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     3901 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag
     3961 tagatgccga ccgaacaaga gctgatttcg agaacgcctc agccagcaac tcgcgcgagc
     4021 ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg gcacagttct cgtccacagt
     4081 tcgctaagct cgctcggctg ggtcgcggga gggccggtcg cagtgattca ggaattaatt
     4141 ccctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     4201 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     4261 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     4321 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     4381 cgcgcggtgt catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc
     4441 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     4501 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     4561 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     4621 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     4681 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     4741 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     4801 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     4861 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     4921 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     4981 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     5041 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     5101 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     5161 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     5221 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     5281 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     5341 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     5401 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     5461 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     5521 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     5581 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     5641 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     5701 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     5761 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     5821 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     5881 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     5941 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     6001 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6781 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6841 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6901 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     6961 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     7021 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7081 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     7141 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     7201 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     7261 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     7321 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     7381 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     7441 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     7501 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     7561 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     7621 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     7681 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     7741 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     7801 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     7861 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     7921 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     7981 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     8041 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     8101 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     8161 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     8221 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     8281 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     8341 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     8401 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     8461 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     8521 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     8581 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     8641 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     8701 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     8761 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     8821 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     8881 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     8941 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     9001 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     9061 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     9121 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     9181 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
     9241 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
     9301 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
     9361 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
     9421 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
     9481 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
     9541 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
     9601 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
     9661 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
     9721 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
     9781 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
     9841 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
     9901 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
     9961 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    10021 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    10081 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    10141 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    10201 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    10261 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    10321 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    10381 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    10441 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    10501 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    10561 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    10621 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    10681 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    10741 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    10801 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    10861 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    10921 aaagtacttt aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    10981 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    11041 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    11101 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg agctctccca
    11161 tatggtcgac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
    11221 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
    11281 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
    11341 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
    11401 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
    11461 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
    11521 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
    11581 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
    11641 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
    11701 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    11761 cagaagacca aagggctat
//
