LOCUS       sequence-255675-        1205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..1205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(184..758,759..761,762..770)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(351..368)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     578..597
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(790..809)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gacggatggc gcgggacctc tcggcatgga cgagctgtac aagtactcag atctcgactt
       61 ctgggaaagg aacctgccct ctgtttctgg cctgctgaag atcattggat tttccacatc
      121 agtaactgcc ctggggtttg tgctgtacaa atacaagctc ctgccacggt cttgaggatc
      181 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
      241 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
      301 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
      361 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
      421 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
      481 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
      541 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
      601 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
      661 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
      721 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga
      781 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
      841 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
      901 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
      961 gggctcgaca tcggcaaggg tgggtcgcgg acgacgcgcc cggtggcgtc tggaccacgc
     1021 cggaaagcgt cgaagcgggg ggccgtgttc cgtagatccg ccgcccatgg cgaaatgaac
     1081 gattcccggt atcccacaac gattggaagg ctcagcgcct acatctagac tgcacgtcga
     1141 tgcataccat gggcacgatg ctcgactgta cacaagtcgt agggatctag gtacacgcga
     1201 tctaa
//
