LOCUS       sequence-255676-       11341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A self-assembling split-GFP based Golden Gate vector to determine 
            protein targeting to plastids in plant cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pLaNGo11-1
FEATURES             Location/Qualifiers
     source          1..11341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1030..1375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1634..2275
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     terminator      2306..3013
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        4030..4375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4505..4810)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4607..4626)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5130..5813)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     5728..5747
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5814..5916)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5947..5994
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    6699..6723
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7003..7632
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8061..9134
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9200..9394
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9456..9560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9561..10355
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10526..11114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11015..11034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11250..11274
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gaattccaat cccacaaaaa
       61 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
      121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
      181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
      241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
      301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
      361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
      421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
      481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
      541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
      601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
      661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
      721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
      781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
      841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
      901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
      961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     1021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     1081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     1141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     1201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     1261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     1321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     1381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     1441 aattacattt acaattatcg ataccatgac caccgctgtc accgccgctg tttctttccc
     1501 ctctaccaaa accacctctc tctccgcccg aagctcctcc gtcatttccc ctgacaaaat
     1561 cagctacaaa aaggttcctt tgtactacag gaatgtatct gcaactggga aaatgggacc
     1621 catcagggcc gcaatgtcca aaggagaaga actgtttacc ggtgttgtgc caattttggt
     1681 tgaactcgat ggtgatgtca acggacataa gttctcagtg agaggcgaag gagaaggtga
     1741 cgccaccatt ggaaaattga ctcttaaatt catctgtact actggtaaac ttcctgtacc
     1801 atggccgact ctcgtaacaa cgcttacgta cggagttcag tgcttttcga gatacccaga
     1861 ccatatgaaa agacatgact tttttaagtc ggctatgcct gaaggttacg tgcaagaaag
     1921 aacaatttcg ttcaaagatg atggaaaata taaaactaga gcagttgtta aatttgaagg
     1981 agatactttg gttaaccgca ttgaactgaa aggaacagat tttaaagaag atggtaatat
     2041 tcttggacac aaactcgaat acaattttaa tagtcataac gtatacatca ctgctgataa
     2101 gcaaaagaac ggaattaaag cgaatttcac agtacgccat aatgtagaag atggcagtgt
     2161 tcaacttgcc gaccattacc aacaaaacac ccctattgga gacggtccgg tacttcttcc
     2221 tgataatcac tacctctcaa cacaaacagt cctgagcaaa gatccaaatg aaaaaggaac
     2281 aggtggcggc ggaagttagc ttgtcctgct ttaatgagat atgcgagaag cctatgatcg
     2341 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc
     2401 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
     2461 tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat
     2521 attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg
     2581 ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga
     2641 accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag
     2701 gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg
     2761 gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta
     2821 ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct
     2881 gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca
     2941 aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt
     3001 cgaggctcag caggaccgct actagaattc gagctcggag gaattccaat cccacaaaaa
     3061 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
     3121 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
     3181 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
     3241 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
     3301 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
     3361 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
     3421 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
     3481 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
     3541 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
     3601 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
     3661 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
     3721 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
     3781 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
     3841 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
     3901 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     3961 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     4021 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     4081 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     4141 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     4201 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     4261 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     4321 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     4381 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
     4441 aattacattt acaattatcg atacaatgtg agaccgactg gctgtgtata agggagcctg
     4501 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     4561 tgagatcagc cacttcttcc ccgataacgg aaaccggcac actggccata tcggtggtca
     4621 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
     4681 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccaggc gtgtcaataa
     4741 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     4801 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
     4861 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
     4921 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
     4981 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
     5041 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
     5101 gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccacgcg gcgtttacgc
     5161 cccccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     5221 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     5281 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     5341 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     5401 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     5461 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     5521 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     5581 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     5641 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     5701 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     5761 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     5821 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     5881 ttatggtgaa agttggaacc tcttacgtgc cgatcaaggt ctcattcgat gggcggcaaa
     5941 ttcatgcgtg accacatggt ccttcatgag tatgtaaatg ctgctgggat tacataagct
     6001 tagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
     6061 cattgcgcac acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt
     6121 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     6181 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     6241 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     6301 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     6361 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
     6421 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
     6481 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
     6541 atcattgctt tgtaattata gttatactca tggatttgta gttgagtatg aaaatatttt
     6601 ttaatgcatt ttatgacttg ccaattgatt gacaacgctt tacgaggatg cacatgtgac
     6661 cgagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta
     6721 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     6781 ttatccgttc gtccatttgt atgtgccagc cgtgcggctg catgaaatcc tggccggttt
     6841 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     6901 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     6961 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     7021 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     7081 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     7141 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     7201 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     7261 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     7321 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     7381 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     7441 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     7501 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     7561 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     7621 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     7681 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     7741 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     7801 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     7861 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     7921 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     7981 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
     8041 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     8101 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     8161 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     8221 aagaatcccg gcaaccgccg gcagccggtg cgtcgtcgat taggaagccg cccaagggcg
     8281 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     8341 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     8401 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     8461 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     8521 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     8581 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     8641 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     8701 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     8761 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     8821 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     8881 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     8941 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     9001 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     9061 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     9121 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     9181 gtcgaaaaag cttctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     9241 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     9301 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     9361 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaac
     9421 agctgcaaaa agcgcccaca tcaaggctcc gagtgcgcgg aacccctatt tgtttatttt
     9481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9541 aatattgaaa aaggaagagt atggctaaaa tgagaatatc accggaattg aaaaaactga
     9601 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
     9661 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
     9721 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     9781 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     9841 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     9901 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     9961 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
    10021 tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag ctgtatgatt
    10081 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
    10141 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
    10201 gggcggacaa gtggtatgac attgccttct gcgtccggtc gctcagggag gatatcgggg
    10261 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
    10321 taaaatatta tattttactg gatgaattgt tttagctgtc agaccaagtt tactcatata
    10381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgctc ggatctgttg
    11161 gaccggacag tagtcatggt tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    11221 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
    11281 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg ttgaacactc
    11341 t
//
