LOCUS       sequence-25568-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E1-8
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(143..163)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(143..161)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(182..198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(182..198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(195..217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      396..987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagacttcat ttcagagaat ggaaggtctc atatacgatg cttttttaat tctttgttag
       61 gcctggtacc tctagagata tcctgcagtg gccaatgcat tggttcgcgg ccgccaccgc
      121 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
      181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcgg gccctgccat agcctcaggt
      241 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      301 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      361 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      541 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      781 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      841 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa acgcctggta
      901 tctttatagt cctgtcgggn ttcgcccccc tctgacttga gcgtcgattt ttgtgatgct
      961 cgtcaggggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggntccng
     1021 ggccttttgn ctggccttt
//
