LOCUS       sequence-255692-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Dsred-blasticidin under CMVpromoter -IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  TITLE     Emory Custom Cloning Core Plasmids - Oskar Laur
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLOC CMV-IRES-Dsred-blasticidin
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2909..3482
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3077..3094)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3304..3323
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3488..4162
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(3668..3688)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3886..3905
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4080..4103
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4712..5300
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4765..4785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5183..5194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5507..5740
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5841..5858)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5847..6071
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6117..6545
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6204..6223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6414..6435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6554..6574)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6559..6888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6739..6874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6801..6820
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     rep_origin      complement(7025..7613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7105..7124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7784..8575,8576..8644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8407..8426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8645..8749)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8797..8931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8834..8853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8888..8907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagatta attaagttta aacttaagct tggtaccgag
     2821 ctcggatcca ctagtccagt gtggtggaat tctgcagata tcacaagttt gtacaaagtg
     2881 gttgctagct aatgaaccgg gcgcgccgcc cctctccctc cccccccccc taacgttact
     2941 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3001 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3061 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3121 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3181 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3241 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3301 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3361 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     3421 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
     3481 taccggtatg gatagcactg agaacgtcat caagcccttc atgcgcttca aggtgcacat
     3541 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gcaagcccta
     3601 cgagggcacc cagaccgcca agctgcaggt gaccaagggc ggccccctgc ccttcgcctg
     3661 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga
     3721 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3781 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacctt
     3841 catctaccac gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
     3901 gaagactctg ggctgggagc cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     3961 gggcgagatc cacaaggcgc tgaagctgaa gggcggcggc cactacctgg tggagttcaa
     4021 gtcaatctac atggccaaga agcccgtgaa gctgcccggc tactactacg tggactccaa
     4081 gctggacatc acctcccaca acgaggacta caccgtggtg gagcagtacg agcgcgccga
     4141 ggcccgccac cacctgttcc agagatctcg agctcaagct tcgaattctg cagtcgagca
     4201 gctgttgaat tttgaccttc ttaagcttgc gggagacgtc gagtccaacc ctgggcccgg
     4261 taccaggagg gccaccatga agaccttcaa catctctcag caggatctgg agctggtgga
     4321 ggtcgccact gagaagatca ccatgctcta tgaggacaac aagcaccatg tcggggcggc
     4381 catcaggacc aagactgggg agatcatctc tgctgtccac attgaggcct acattggcag
     4441 ggtcactgtc tgtgctgaag ccattgccat tgggtctgct gtgagcaacg ggcagaagga
     4501 ctttgacacc attgtggctg tcaggcaccc ctactctgat gaggtggaca gatccatcag
     4561 ggtggtcagc ccctgtggca tgtgcagaga gctgatctct gactatgctc ctgactgctt
     4621 tgtgctcatt gagatgaatg gcaagctggt caaaaccacc attgaggaac tcatccccct
     4681 caagtacacc aggaactaat aataagttaa caatcaacct ctggattaca aaatttgtga
     4741 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4801 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4861 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4921 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4981 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5041 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5101 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5161 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5221 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5281 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     5341 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5401 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5461 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     5521 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     5581 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5641 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5701 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     5761 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     5821 tgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5881 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5941 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6001 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6061 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     6121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6541 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     6601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     6841 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6901 gcttgtatat ccattttcgg atctgatcag cacgtgatga aaaagcctga actcaccgcg
     6961 acgtcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8761 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
     8821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8881 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     8941 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     9001 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     9061 aattgtattt gttaaatatg tactacaaac ttagtagt
//
