LOCUS       sequence-255703-        9101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Chinthalapudi K, Rangarajan ES, Patil DN, George EM, Brown DT, Izard
            T
  TITLE     Lipid binding promotes oligomerization and focal adhesion activity 
            of vinculin.
  JOURNAL   J Cell Biol. 2014 Dec 8;207(5):643-56. doi: 10.1083/jcb.201404128.
  PUBMED    25488920
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-12-8- 8"; volume: "207"; 
            issue: "5"; pages: "643-56"
COMMENT     Sequence Label: pcDNA4-GFP-Vinculin
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    659..677
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    680..698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     708..732
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             838..867
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(868..870,871..873,874..1584)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(868..870,871..873,874..1584)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(913..934)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1174..1193)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1521..1542
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1591..1614
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4863..4892
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4908..4925
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4948..4965)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4954..5178
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5224..5652
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5311..5330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5521..5542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5661..5681)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5666..5995
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5846..5981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5908..5927
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6043..6090
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6109..6483
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6613..6734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6650..6669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6704..6723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6783..6799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6783..6799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6796..6818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6807..6823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6831..6837,6838..6855,6856..6861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6876..6897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7014..7031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7185..7773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7265..7284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7944..8735,8736..8804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8567..8586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8805..8909)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8984..9003)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      661 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
      721 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      781 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttgctagcgg taccatgcat
      841 caccaccacc atcatcatca ccaccatatg gtgagcaagg gcgaggagct gttcaccggg
      901 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      961 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1021 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1081 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1141 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1201 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1261 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1321 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1381 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1441 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1501 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcacc
     1561 ctcggcatgg acgagctgta caagggatcc ctggaagttc tgttccaggg gcccgccgcc
     1621 gctatgccag tgtttcatac gcgcacgatc gagagcatcc tggagccggt ggcacagcag
     1681 atctcccacc tggtgataat gcacgaggag ggcgaggtgg acggcaaagc cattcctgac
     1741 ctcaccgcgc ccgtggccgc cgtgcaggcg gccgtcagca acctcgtccg ggttggaaaa
     1801 gagactgttc aaaccactga ggatcagatt ttgaagagag atatgccacc agcatttatt
     1861 aaggttgaga atgcttgcac caagcttgtc caggcagctc agatgcttca gtcagaccct
     1921 tactcagtgc ctgctcgaga ttatctaatt gatgggtcaa ggggcatcct ctctggaaca
     1981 tcagacctgc tccttacctt cgatgaggct gaggtccgta aaattattag agtttgcaaa
     2041 ggaattttgg aatatcttac agtggcagag gtggtggaga ctatggaaga tttggtcact
     2101 tacacaaaga atcttgggcc aggaatgact aagatggcca agatgattga cgagagacag
     2161 caggagctca ctcaccagga gcaccgagtg atgttggtga actcgatgaa caccgtgaaa
     2221 gagttgctgc cagttctcat ttcagctatg aagatttttg taacaactaa aaactcaaaa
     2281 aaccaaggca tagaggaagc tttaaaaaat cgcaatttta ctgtagaaaa aatgagtgct
     2341 gaaattaatg agataattcg tgtgttacaa ctcacctctt gggatgaaga tgcctgggcc
     2401 agcaaggaca ctgaagccat gaagagagca ttggcctcca tagactccaa actgaaccag
     2461 gccaaaggtt ggctccgtga ccctagtgcc tccccagggg atgctggtga gcaggccatc
     2521 agacagatct tagatgaagc tggaaaagtt ggtgaactct gtgcaggcaa agaacgcagg
     2581 gagattctgg gaacttgcaa aatgctaggg cagatgactg atcaagtggc tgacctccgt
     2641 gccagaggac aaggatcctc accggtggcc atgcagaaag ctcagcaggt atctcagggt
     2701 ctggatgtgc tcacagcaaa agtggaaaat gcagctcgca agctggaagc catgaccaac
     2761 tcaaagcaga gcattgcaaa gaagatcgat gctgctcaga actggcttgc agatccaaat
     2821 ggtggaccgg aaggagaaga gcagattcga ggtgctttgg ctgaagctcg gaaaatagca
     2881 gaattatgtg atgatcctaa agaaagagat gacattctac gttcccttgg ggaaatatct
     2941 gctctgactt ctaaattagc agatctacga agacagggga aaggagattc tccagaggct
     3001 cgagccttgg ccaaacaggt ggccacggcc ctgcagaacc tgcagaccaa aaccaaccgg
     3061 gctgtggcca acagcagacc ggccaaagca gctgtacacc ttgagggcaa gattgagcaa
     3121 gcacagcggt ggattgataa tcccacagtg gatgaccgtg gagtcggtca ggctgccatc
     3181 cgggggcttg tggccgaagg gcatcgtctg gctaatgtta tgatggggcc ttatcggcaa
     3241 gatcttctcg ccaagtgtga ccgagtggac cagctgacag cccagctggc tgacctggct
     3301 gccagagggg aaggggagag tcctcaggca cgagcacttg catctcagct ccaagactcc
     3361 ttaaaggatc taaaagctcg gatgcaggag gccatgactc aggaagtgtc agatgttttc
     3421 agcgatacca caactcccat caagctgttg gcagtggcag ccacggcgcc tcctgatgcg
     3481 cctaacaggg aagaggtatt tgatgagagg gcagctaact ttgaaaacca ttcaggaaag
     3541 cttggtgcta cggccgagaa ggcggctgcg gttggtactg ctaataaatc aacagtggaa
     3601 ggcattcagg cctcagtgaa gacggcccga gaactcacac cccaggtggt ctcggctgct
     3661 cgtatcttac ttaggaaccc tggaaatcaa gctgcttatg aacattttga gaccatgaag
     3721 aaccagtgga tcgataatgt tgaaaaaatg acagggctgg tggacgaagc cattgatacc
     3781 aaatctctgt tggatgcttc agaagaagca attaaaaaag acctggacaa gtgcaaggta
     3841 gctatggcca acattcagcc tcagatgctg gttgctgggg caaccagtat tgctcgtcgg
     3901 gccaaccgga tcctgctggt ggctaagagg gaggtggaga attccgagga tcccaagttc
     3961 cgtgaggctg tgaaagctgc ctctgatgaa ttgagcaaaa ccatctcccc aatggtgatg
     4021 gatgcaaaag ctgtggctgg aaacatttcc gaccctggac tgcaaaagag cttcctggac
     4081 tcaggatatc ggatcctggg agctgtggcc aaggtcagag aagccttcca acctcaggag
     4141 cctgacttcc cgccgcctcc accagacctt gaacaactcc gactaacaga tgagcttgct
     4201 cctcccaaac cacctctgcc tgaaggtgag gtccctccac ctaggcctcc accaccagag
     4261 gaaaaggatg aagagttccc tgagcagaag gccggggagg tgattaacca gccaatgatg
     4321 atggctgcca gacagctcca tgatgaagct cgcaaatggt ccagcaaggg caatgacatc
     4381 attgcagcag ccaagcgcat ggctctgctg atggctgaga tgtctcggct ggtaagaggg
     4441 ggcagtggta ccaagcgggc actcattcag tgtgccaagg acatcgccaa ggcctcagat
     4501 gaggtgactc ggttggccaa ggaggttgcc aagcagtgca cagataaacg gattagaacc
     4561 aacctcttac aggtatgtga gcgaatccca accataagca cccagctcaa aatcctgtcc
     4621 acagtgaagg ccaccatgct gggccggacc aacatcagtg atgaggagtc tgagcaggcc
     4681 acagagatgc tggttcacaa tgcccagaac ctcatgcagt ctgtgaagga gactgtgcgg
     4741 gaagctgaag ctgcttcaat caaaattcga acagatgctg gatttacact gcgctgggtt
     4801 agaaagactc cctggtacca gtgagaattc gcggccgctc gagtctagag ggcccgcggt
     4861 tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc
     4921 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4981 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5041 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5101 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5161 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     5221 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5401 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5641 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5701 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5761 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5821 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5881 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6001 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     6061 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     6121 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     6181 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     6241 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     6301 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     6361 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     6421 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     6481 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6541 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6601 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6661 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6721 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6781 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6841 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7141 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7201 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7261 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7321 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7381 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7441 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7501 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7561 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7621 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7681 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7741 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7801 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7861 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7921 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7981 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8041 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8101 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8161 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8221 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8281 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8341 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8401 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8461 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8521 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8581 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8641 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8701 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8761 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8821 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8881 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8941 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     9001 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     9061 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga c
//
