LOCUS       sequence-255717-        8624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human NLRP11 188A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Ellwanger K, Becker E, Kienes I, Sowa A, Postma Y, Gloria YC, Weber 
            ANR, Kufer TA
  TITLE     The NLR family pyrin domain containing 11 protein contributes to the
            regulation of inflammatory signalling.
  JOURNAL   J Biol Chem. 2018 Jan 4. pii: RA117.000152. doi: 
            10.1074/jbc.RA117.000152.
  PUBMED    29301940
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Jan 4. pii: RA117.000152. doi: 10.1074/jbc.RA117.000152."
COMMENT     Sequence Label: pNLRP11minor.MYC
FEATURES             Location/Qualifiers
     source          1..8624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             689..718
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             722..751
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             755..784
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             788..811
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(3981..3998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3987..4211
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4257..4685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4344..4363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4554..4575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4694..4714)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4699..5028
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4879..5014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4941..4960
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5095..5889
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5149..5168)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5759..5778
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6063..6184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6100..6119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6154..6173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6233..6249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6233..6249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6246..6268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6257..6273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6281..6287,6288..6305,6306..6311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6326..6347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6464..6481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6635..7223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6715..6734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7394..8185,8186..8254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8017..8036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8255..8359)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8434..8453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttaccatgga gcagaagctg atctccgagg aggacctgaa
      721 cgagcagaag ctgatctccg aggaggacct gaacgagcag aagctgatct ccgaggagga
      781 cctgaacctg gaggtgctgt tccagggacc cggggtacca ggatccagtg tggtggaatt
      841 catggcagaa tcggattcta ctgactttga cctgctgtgg tatctagaga atctcagtga
      901 caaggaattt cagagtttta agaagtatct ggcacgcaag attcttgatt tcaaactgcc
      961 acagtttcca ctgatacaga tgacaaaaga agaactggct aacgtgttgc caatctctta
     1021 tgagggacag tatatatgga atatgctctt cagcatattt tcaatgatgc gtaaggaaga
     1081 tctttgtagg aagatcattg gcagacgaaa ccgcaatcag gaggcatgca aagctgtcat
     1141 gaggagaaaa ttcatgctgc aatgggaaag tcacactttt ggaaaatttc attataaatt
     1201 ttttcgtgac gtttcgtcag atgtgttcta catacttcaa ttagcctatg attctaccag
     1261 ctattattca gcaaacaatc tcaatgtgtt cctgatggga gagagagcat ctggaaaaac
     1321 tattgttata aatctggctg tgttgaggtg gatcaagggt gagatgtggc agaacatgat
     1381 ctcgtacgtc gttcacctca ctgctcacga aataaaccag atgaccaaca gcagcttggc
     1441 tgagctaatc gccaaggact ggcctgacgg ccaggctccc attgcagaca tcctgtctga
     1501 tcccaagaaa ctccttttca tcctcgagga cttggacaac ataagattcg agttaaatgt
     1561 caatgaaagt gctttgtgta gtaacagcac ccagaaagtt cccattccag ttctcctggt
     1621 cagtttgctg aagagaaaaa tggctccagg ctgctggttc ctcatctcct caaggcccac
     1681 acgtgggaat aatgtaaaaa cgttcttgaa agaggtagat tgctgcacga ccttgcagct
     1741 gtcgaatggg aagagggaga tatattttaa ctctttcttt aaagaccgcc agagggcgtc
     1801 ggcagccctc cagcttgtac atgaggatga aatactcgtg ggtctgtgcc gagtcgccat
     1861 cttatgctgg atcacgtgta ctgtcctgaa gcggcagatg gacaaggggc gtgacttcca
     1921 gctctgctgc caaacaccca ctgatctaca tgcccacttt cttgctgatg cgttgacatc
     1981 agaggctgga cttactgcca atcagtatca cctaggtctc ctaaaacgtc tgtgtttgct
     2041 ggctgcagga ggactgtttc tgagcaccct gaatttcagt ggtgaagacc tcagatgtgt
     2101 tgggtttact gaggctgatg tctctgtgtt gcaggccgcg aatattcttt tgccgagcaa
     2161 cactcataaa gaccgttaca agttcataca cttgaacgtc caggagtttt gtacagccat
     2221 tgcatttctg atggcagtac ccaactatct gatcccctca ggcagcagag agtataaaga
     2281 gaagagagaa caatactctg actttaatca agtgtttact ttcatttttg gtcttctaaa
     2341 tgcaaacagg agaaagattc ttgagacatc ctttggatac cagctaccga tggtagacag
     2401 cttcaagtgg tactcggtgg gatacatgaa acatttggac cgtgacccgg aaaagttgac
     2461 gcaccatatg cctttgtttt actgtctcta tgagaatcgg gaagaagaat ttgtgaagac
     2521 gattgtggat gctctcatgg aggttacagt ttaccttcaa tcagacaagg atatgatggt
     2581 ctcattatac tgtctggatt actgctgtca cctgaggaca cttaagttga gcgttcagcg
     2641 catctttcaa aacaaagagc cacttataag gccaactgct agtcaaatga agagccttgt
     2701 ctactggaga gagatctgct ctctttttta tacaatggag agcctccggg agctgcatat
     2761 ctttgacaat gaccttaatg gtatttcaga aaggattctg tctaaagccc tggagcattc
     2821 tagctgtaaa cttcgcacac tcaagttgtc ctatgtctcg actgcttctg gttttgaaga
     2881 cttactcaag gctttggctc gtaatcggag cctgacatac ctgagtatca actgtacgtc
     2941 catttcccta aatatgtttt cacttctgca tgacatcctg cacgagccca catgccaaat
     3001 aagtcatctg agcttgatga aatgtgattt gcgagccagc gaatgcgaag aaatcgcctc
     3061 tctcctcatc agtggcggga gtctgagaaa actgacctta tccagcaatc cgctgaggag
     3121 cgacgggatg aacatactgt gtgatgcctt gcttcatccc aactgcactc ttatatcact
     3181 ggtgttagtc ttctgctgtc tcactgaaaa ttgctgcagc gcccttggaa gagtgcttct
     3241 gttcagccca actctaagac aactagacct gtgtgtgaat cgcttaaaaa attacggagt
     3301 gttgcatgtg acgtttccct tgctgtttcc aacctgtcag ttagaggagc ttcatctgtc
     3361 tggctgtttc tttagcagcg atatctgtca atatattgcc atagttattg ctactaatga
     3421 aaaactgagg agcctggaga ttgggagcaa caaaatagaa gatgcaggaa tgcagctgct
     3481 atgtggtggt ttgagacatc ccaactgcat gttggtgaat attgggctag aagagtgcat
     3541 gttaaccagt gcctgctgtc gatctcttgc ctctgttctt accaccaaca aaacactaga
     3601 aagactcaac ttgcttcaaa atcacttggg caatgatgga gttgcaaaac ttcttgagag
     3661 cttgatcagc ccagattgtg tacttaaggt agttgggctt ccattaactg gcctgaacac
     3721 acaaacccag cagttgctga tgactgtaaa ggaaagaaaa cccagtttga tctttctgtc
     3781 tgaaacttgg tctttaaagg aaggcagaga aattggtgtg acacctgctt ctcagccagg
     3841 ttcaataata cctaattcta atttggatta catgtttttc aaatttccca gaatgtctgc
     3901 agccatgaga acgtcaaata cagcatctag gcaacccctt tgagtcgagt ctagagggcc
     3961 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4021 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4081 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4141 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4201 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4261 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4321 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4381 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4441 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4501 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4561 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4621 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4681 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4741 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4801 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4861 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4921 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4981 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5041 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5101 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5161 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5221 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5281 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5341 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5401 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5461 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5521 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5581 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5641 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5701 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5761 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5821 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5881 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5941 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6001 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6061 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6181 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8401 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8461 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     8521 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     8581 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
