LOCUS       sequence-25572-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E2-2
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(141..161)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(141..159)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(180..196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(180..196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(193..215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gnttcagatt cagagatcat tgaaagtcgt cagcattgct ttttnaattc tttgttaggc
       61 ctggtacctc tagagatatc ctgcagtggc caatgcattg gttcgcggcc gccaccgcgg
      121 tggagctcca gctttngttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
      181 tcatagctgt ttcctgtgtg aaattgttat ccgctcgggc cctgccatag cctcaggtta
      241 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
      301 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
      361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      541 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcgaagtacc gancgaggcg
      841 angaaccnag annnnngaac nggngagcgc acgagggagc ttccaggggg aaacgccngg
      901 gnatctttan agnncctggt cggnnnntgc ncnncctctg ancttggagc gtcngatttt
      961 ttgggnaggc ccngncangg gggggcggga gccctatggg aaaaaacgcc nagcnaccgc
     1021 gggccttttt ttacggntnc cngggccttt tt
//
