LOCUS       sequence-255727-        9317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP 
            linked via P2A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Shao Z, Zhang R, Khodadadi-Jamayran A, Chen B, Crowley MR, Festok 
            MA, Crossman DK, Townes TM, Hu K
  TITLE     The acetyllysine reader BRD3R promotes human nuclear reprogramming 
            and regulates mitosis.
  JOURNAL   Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
  PUBMED    26947130
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-7"; volume: "7:10869. doi"; pages: " 
            10.1038/ncomms10869"
COMMENT     Sequence Label: pLVH-EF1a-GFP-P2A-OCT4
FEATURES             Location/Qualifiers
     source          1..9317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2409..3347
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3304..3324
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3364..3373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3370..4086
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             4114..4170
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5285..5873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5338..5358)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5756..5767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6080..6713
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6842..6858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6842..6858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6855..6877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6866..6882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6890..6896,6897..6914,6915..6920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6935..6956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7073..7090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7244..7832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7324..7343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8003..8794,8795..8863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8626..8645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8864..8968)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9016..9150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9053..9072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9107..9126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2461 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2521 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2581 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2641 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2701 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2761 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2821 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2881 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2941 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3001 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3061 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3121 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3181 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3241 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3301 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacccg
     3361 gtcgccacca tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
     3421 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
     3481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
     3541 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
     3601 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
     3661 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc
     3721 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc
     3781 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag
     3841 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg
     3901 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     3961 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     4021 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     4081 tacaagtccg gactcagatc tcgaggatct ggagcaacaa acttctcact actcaaacaa
     4141 gcaggtgacg tggaggagaa tcccggcccc atggcgggac acctggcttc ggatttcgcc
     4201 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt
     4261 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg
     4321 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt
     4381 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg
     4441 gagacctctc agcctgaggg tgaagcagga gtcggggtgg agagcaactc cgatggggcc
     4501 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag
     4561 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc
     4621 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc
     4681 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg
     4741 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa
     4801 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga
     4861 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg
     4921 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag
     4981 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc
     5041 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg
     5101 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc
     5161 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc
     5221 accactctgg gctctcccat gcattcaaac tgataaggat ccaccggatc taggcgtctg
     5281 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5341 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5401 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5461 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5521 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5581 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5641 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     5701 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5761 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5821 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     5881 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     5941 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6001 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6061 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     6121 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     6181 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     6241 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     6301 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     6361 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     6421 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     6481 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     6541 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6601 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6661 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6721 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6781 gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat
     6841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9001 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9121 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     9181 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     9241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9301 actacaaact tagtagt
//
