LOCUS       sequence-255744-       10078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen.
            Lenti Virus destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0455
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    42..630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(95..115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(513..524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(633..649)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(634..650)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             740..973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(998..1016)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(999..1016)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1306..1325)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1539..1558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1718..1736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1804..1908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2146..2961)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2146..2961)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2869..2888
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2953..2972
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3186..3774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3675..3694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4021..4038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4108..4128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4113..4442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4293..4428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5576..5641
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5771..5791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6216..6350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6253..6272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6307..6326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6518..7151
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7198..7323
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7820..8053
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8238..8282
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8567..8684
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8781..9291
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9210..9228
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9296..9322
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             9329..10024
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
ORIGIN
        1 gttacagaga tgttacgaac cactagtgca ctgcagtaca taatcaacct ctggattaca
       61 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
      121 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
      181 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
      241 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
      301 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
      361 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
      421 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
      481 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
      541 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
      601 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat
      661 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
      721 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
      781 tttgcttgta ctggttctct ctggttagac cagatctgag cctgggagct ctctggctaa
      841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      961 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
     1021 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
     1081 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
     1141 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
     1201 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
     1261 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     1321 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     1381 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     1441 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     1501 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
     1561 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1621 ctcccggcat ccgcttacag acaagctgtg accgtctacg ggagctgcat gtgtcagagg
     1681 ttttcaccgt catcaccgaa acgcgcgaaa cgaaagggcc tcgtgatacg cctattttta
     1741 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1801 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1861 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1921 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1981 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2041 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2101 ccaatgccga gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc
     2161 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     2221 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     2281 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     2341 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     2401 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     2461 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg
     2521 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     2581 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     2641 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     2701 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     2761 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     2821 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     2881 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt
     2941 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     3001 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     3061 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcagt catgaccaaa
     3121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3841 cctgcgttat cccctgattc tgtggataac cgtgcggccg ccaatttttt acggttcctg
     3901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4021 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4081 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4141 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4201 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4261 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4321 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4381 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4441 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     4501 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     4561 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     4621 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     4681 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     4741 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     4801 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     4861 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     4921 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     4981 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     5041 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     5101 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
     5161 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
     5221 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
     5281 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
     5341 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
     5401 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
     5461 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
     5521 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     5581 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     5641 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     5701 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     5761 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     5821 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     5881 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     5941 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     6001 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     6061 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     6121 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     6181 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     6241 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6301 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     6361 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     6421 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     6481 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
     6541 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     6601 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     6661 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     6721 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     6781 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     6841 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     6901 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     6961 tgcctgtact gggactctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7021 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7081 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7141 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7201 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7261 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7321 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7381 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7441 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7501 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7561 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7621 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     7681 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7741 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7801 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7861 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7921 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7981 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8041 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8101 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8161 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8221 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8281 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8341 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8401 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8461 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8521 tgaacggatc tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag
     8581 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     8641 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     8701 acagcagaga tccagtttgg ccctgaattc gcatctagac tgaactggcc gataattgca
     8761 gacgaacgtc gaattcccac ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     8821 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     8881 tcttgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     8941 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     9001 gcgtgccgga cgtgacaaac ggaagccgca cgtgtcacta gtaccctcgc agacggacag
     9061 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     9121 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     9181 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     9241 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gtatgctgcc
     9301 ccccctggag aggctgaccc tgggttctag cgagctgatt aaggagaaca tgcacatgaa
     9361 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
     9421 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     9481 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
     9541 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
     9601 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     9661 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
     9721 gatgcagaag aaaacactcg gctgggaggc cttcaccgaa acgctgtacc ccgctgacgg
     9781 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
     9841 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
     9901 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaaa cctacgtcga
     9961 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
    10021 taatggatcc taaactagag atctatgtga ggatcctaac tcgagatcga gctgagca
//
