LOCUS       sequence-255749-       12529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLT1 G886V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FLT1-G886V
FEATURES             Location/Qualifiers
     source          1..12529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7466..7490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7543..7584
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7605..8172
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7622..8174
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7766..7783)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7993..8012
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8186..8188,8189..8191,8192..8905)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8186..8188,8189..8191,8192..8902)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8231..8252)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8492..8511)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8839..8860
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8919..9507
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8972..8992)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9390..9401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9582..9815
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9934..9952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10020..10124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10125..10193,10194..10985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10343..10362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11156..11744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11645..11664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11898..11915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12155..12176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12191..12196,12197..12214,12215..12221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12229..12245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12234..12256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12253..12269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12253..12269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12319..12338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gtcagctact gggacaccgg ggtcctgctg
     3481 tgcgcgctgc tcagctgtct gcttctcaca ggatctagtt caggttcaaa attaaaagat
     3541 cctgaactga gtttaaaagg cacccagcac atcatgcaag caggccagac actgcatctc
     3601 caatgcaggg gggaagcagc ccataaatgg tctttgcctg aaatggtgag taaggaaagc
     3661 gaaaggctga gcataactaa atctgcctgt ggaagaaatg gcaaacaatt ctgcagtact
     3721 ttaaccttga acacagctca agcaaaccac actggcttct acagctgcaa atatctagct
     3781 gtacctactt caaagaagaa ggaaacagaa tctgcaatct atatatttat tagtgataca
     3841 ggtagacctt tcgtagagat gtacagtgaa atccccgaaa ttatacacat gactgaagga
     3901 agggagctcg tcattccctg ccgggttacg tcacctaaca tcactgttac tttaaaaaag
     3961 tttccacttg acactttgat ccctgatgga aaacgcataa tctgggacag tagaaagggc
     4021 ttcatcatat caaatgcaac gtacaaagaa atagggcttc tgacctgtga agcaacagtc
     4081 aatgggcatt tgtataagac aaactatctc acacatcgac aaaccaatac aatcatagat
     4141 gtccaaataa gcacaccacg cccagtcaaa ttacttagag gccatactct tgtcctcaat
     4201 tgtactgcta ccactccctt gaacacgaga gttcaaatga cctggagtta ccctgatgaa
     4261 aaaaataaga gagcttccgt aaggcgacga attgaccaaa gcaattccca tgccaacata
     4321 ttctacagtg ttcttactat tgacaaaatg cagaacaaag acaaaggact ttatacttgt
     4381 cgtgtaagga gtggaccatc attcaaatct gttaacacct cagtgcatat atatgataaa
     4441 gcattcatca ctgtgaaaca tcgaaaacag caggtgcttg aaaccgtagc tggcaagcgg
     4501 tcttaccggc tctctatgaa agtgaaggca tttccctcgc cggaagttgt atggttaaaa
     4561 gatgggttac ctgcgactga gaaatctgct cgctatttga ctcgtggcta ctcgttaatt
     4621 atcaaggacg taactgaaga ggatgcaggg aattatacaa tcttgctgag cataaaacag
     4681 tcaaatgtgt ttaaaaacct cactgccact ctaattgtca atgtgaaacc ccagatttac
     4741 gaaaaggccg tgtcatcgtt tccagacccg gctctctacc cactgggcag cagacaaatc
     4801 ctgacttgta ccgcatatgg tatccctcaa cctacaatca agtggttctg gcacccctgt
     4861 aaccataatc attccgaagc aaggtgtgac ttttgttcca ataatgaaga gtcctttatc
     4921 ctggatgctg acagcaacat gggaaacaga attgagagca tcactcagcg catggcaata
     4981 atagaaggaa agaataagat ggctagcacc ttggttgtgg ctgactctag aatttctgga
     5041 atctacattt gcatagcttc caataaagtt gggactgtgg gaagaaacat aagcttttat
     5101 atcacagatg tgccaaatgg gtttcatgtt aacttggaaa aaatgccgac ggaaggagag
     5161 gacctgaaac tgtcttgcac agttaacaag ttcttataca gagacgttac ttggatttta
     5221 ctgcggacag ttaataacag aacaatgcac tacagtatta gcaagcaaaa aatggccatc
     5281 actaaggagc actccatcac tcttaatctt accatcatga atgtttccct gcaagattca
     5341 ggcacctatg cctgcagagc caggaatgta tacacagggg aagaaatcct ccagaagaaa
     5401 gaaattacaa tcagagatca ggaagcacca tacctcctgc gaaacctcag tgatcacaca
     5461 gtggccatca gcagttccac cactttagac tgtcatgcta atggtgtccc cgagcctcag
     5521 atcacttggt ttaaaaacaa ccacaaaata caacaagagc ctggaattat tttaggacca
     5581 ggaagcagca cgctgtttat tgaaagagtc acagaagagg atgaaggtgt ctatcactgc
     5641 aaagccacca accagaaggg ctctgtggaa agttcagcat acctcactgt tcaaggaacc
     5701 tcggacaagt ctaatctgga gctgatcact ctaacatgca cctgtgtggc tgcgactctc
     5761 ttctggctcc tattaaccct ctttatccga aaaatgaaaa ggtcttcttc tgaaataaag
     5821 actgactacc tatcaattat aatggaccca gatgaagttc ctttggatga gcagtgtgag
     5881 cggctccctt atgatgccag caagtgggag tttgcccggg agagacttaa actgggcaaa
     5941 tcacttggaa gaggggcttt tggaaaagtg gttcaagcat cagcatttgg cattaagaaa
     6001 tcacctacgt gccggactgt ggctgtgaaa atgctgaaag agggggccac ggccagcgag
     6061 tacaaagctc tgatgactga gctaaaaatc ttgacccaca ttgtccacca tctgaacgtg
     6121 gttaacctgc tgggagcctg caccaagcaa ggagggcctc tgatggtgat tgttgaatac
     6181 tgcaaatatg gaaatctctc caactacctc aagagcaaac gtgacttatt ttttctcaac
     6241 aaggatgcag cactacacat ggagcctaag aaagaaaaaa tggagccagg cctggaacaa
     6301 ggcaagaaac caagactaga tagcgtcacc agcagcgaaa gctttgcgag ctccggcttt
     6361 caggaagata aaagtctgag tgatgttgag gaagaggagg attctgacgg tttctacaag
     6421 gagcccatca ctatggaaga tctgatttct tacagttttc aagtggccag aggcatggag
     6481 ttcctgtctt ccagaaagtg cattcatcgg gacctggcag cgagaaacat tcttttatct
     6541 gagaacaacg tggtgaagat ttgtgatttt ggccttgccc gggatattta taagaacccc
     6601 gattatgtga gaaaaggaga tactcgactt cctctgaaat ggatggctcc tgaatctatc
     6661 tttgacaaaa tctacagcac caagagcgac gtgtggtctt acggagtatt gctgtgggaa
     6721 atcttctcct taggtgggtc tccataccca ggagtacaaa tggatgagga cttttgcagt
     6781 cgcctgaggg aaggcatgag gatgagagct cctgagtact ctactcctga aatctatcag
     6841 atcatgctgg actgctggca cagagaccca aaagaaaggc caagatttgc agaacttgtg
     6901 gaaaaactag gtgatttgct tcaagcaaat gtacaacagg atggtaaaga ctacatccca
     6961 atcaatgcca tactgacagg aaatagtggg tttacatact caactcctgc cttctctgag
     7021 gacttcttca aggaaagtat ttcagctccg aagtttaatt caggaagctc tgatgatgtc
     7081 agatacgtaa atgctttcaa gttcatgagc ctggaaagaa tcaaaacctt tgaagaactt
     7141 ttaccgaatg ccacctccat gtttgatgac taccagggcg acagcagcac tctgttggcc
     7201 tctcccatgc tgaagcgctt cacctggact gacagcaaac ccaaggcctc gctcaagatt
     7261 gacttgagag taaccagtaa aagtaaggag tcggggctgt ctgatgtcag caggcccagt
     7321 ttctgccatt ccagctgtgg gcacgtcagc gaaggcaagc gcaggttcac ctacgaccac
     7381 gctgagctgg aaaggaaaat cgcgtgctgc tccccgcccc cagactacaa ctcggtggtc
     7441 ctgtactcca ccccacccat ctagaaccca gctttcttgt acaaagtggt tgatatccag
     7501 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     7561 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc ccccccccct
     7621 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     7681 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     7741 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     7801 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     7861 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     7921 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     7981 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     8041 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     8101 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     8161 acacgatgat aatatggcca cacatatggt gagcaagggc gaggagctgt tcaccggggt
     8221 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     8281 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     8341 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     8401 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     8461 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     8521 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     8581 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     8641 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     8701 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     8761 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     8821 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     8881 cggcatggac gagctgtaca agtaaatcga tagatcctaa tcaacctctg gattacaaaa
     8941 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9001 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9061 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9121 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9181 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9241 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9301 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     9361 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9421 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9481 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     9541 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     9601 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     9661 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9721 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9781 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9841 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     9901 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     9961 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
    10381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11821 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11881 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11941 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    12001 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    12061 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    12121 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12181 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12241 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    12301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    12361 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    12421 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12481 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
