LOCUS       sequence-255751-        1165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tagless S3-M129C mutant ribosomal protein in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
  TITLE     Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA 
            Folding.
  JOURNAL   Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 
            10.1016/j.cell.2019.10.035. Epub 2019 Nov 21.
  PUBMED    31761533
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.035"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1357-1369.e16"
COMMENT     Sequence Label: pRSF-1b-S3-M129C
FEATURES             Location/Qualifiers
     source          1..1165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(776..794)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      790..837
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggcggtacat tccctgtaga ataattttgt ttaactttaa taaggagata taccatgggt
       61 cagaaagtac atcctaatgg tattcgcctg ggtattgtaa aaccatggaa ctctacctgg
      121 tttgcgaaca ccaaagaatt cgctgacaac ctggacagcg attttaaagt acgtcagtac
      181 ctgactaagg aactggctaa agcgtccgta tctcgtatcg ttatcgagcg tccggctaag
      241 agcatccgtg taaccattca cactgctcgc ccgggtatcg ttatcggtaa aaaaggtgaa
      301 gacgtagaaa aactgcgtaa ggtcgtagcg gacatcgctg gcgttcctgc acagatcaac
      361 atcgccgaag ttcgtaagcc tgaactggac gcaaaactgg ttgctgacag catcacttct
      421 cagctggaac gtcgcgtttg cttccgtcgt gctatgaagc gtgctgtaca gaacgcaatg
      481 cgtctgggcg ctaaaggtat taaagttgaa gttagcggcc gtctgggcgg cgcggaaatc
      541 gcacgtaccg aatggtaccg cgaaggtcgc gtaccgctgc acactctgcg tgctgacatc
      601 gactacaaca cctctgaagc gcacaccact tacggtgtaa tcggcgttaa agtgtggatc
      661 ttcaaaggcg agatcctggg tggtatggct gctgttgaac aaccggaaaa accggctgct
      721 cagcctaaaa agcagcagcg taaaggccgt aaataattaa cctaggctgc tgccaccgct
      781 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      841 aaacctcacg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
      901 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gacagctgac
      961 gaccgggtct ccgcaagtgg cacttttcgg ggaaatgtgc gcgaccctca gtggtaattc
     1021 ctaaatacat tccaatatgt atccgctcat gaattaatgc ttacgaaaac tcatcgagca
     1081 tcaaatgaac tgcatttatc atatcagata tcatacatat ttttggaaaa gcgttctgta
     1141 tgagaaaaac tcaccgagca gtcct
//
