LOCUS       sequence-255752-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tagless S15-T79C mutant ribosomal protein in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Duss O, Stepanyuk GA, Puglisi JD, Williamson JR
  TITLE     Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA 
            Folding.
  JOURNAL   Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 
            10.1016/j.cell.2019.10.035. Epub 2019 Nov 21.
  PUBMED    31761533
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.035"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1357-1369.e16"
COMMENT     Sequence Label: pRSF-1b-S15-T79C
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(347..365)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      361..408
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttaacggta cattccctgt agaataattt tgtttaactt taataaggag atataccatg
       61 tctctaagta ctgaagcaac agctaaaatc gtttctgagt ttggtcgtga cgcaaacgac
      121 accggttcta ccgaagttca ggtagcactg ctgactgcac agatcaacca cctgcagggc
      181 cactttgcag agcacaaaaa agatcaccac agccgtcgtg gtctgctgcg catggtttct
      241 cagcgtcgta aactgctcga ctacctgaaa cgtaaagacg tagcacgtta ctgccagctc
      301 atcgagcgcc tgggtctgcg tcgctaatta acctaggctg ctgccaccgc tgagcaataa
      361 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaacctcag
      421 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
      481 tagacataag cggctattta acgaccctgc cctgaaccga cgacaagctg acgaccgggt
      541 ctccgcaagt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      601 aatacattca aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca
      661 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
      721 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
      781 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaat
      841 aaagttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      901 tttatgcatt tctttccaga cttgttcaac agggccagcc attacgctcg tcatcaaaat
      961 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     1021 ggtcgctgtt aaggacatta caaacaggat cgaatgcaac ggggcaggaa cactgcaagc
     1081 gcatcaacaa aattttcacc tggaatcagg aaatcctctg
//
