LOCUS       sequence-25576-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E2-4
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(146..166)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(146..164)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(185..201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(185..201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(198..220)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 agggangaga tctattcaga gaataatctc tatacaccac ttcctttttt aattctttgt
       61 taggcctggt acctctagag atatcctgca gtggccaatg cattggttcg cggccgccac
      121 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      181 catggtcata gctgtttcct gtgtgaaatt gttatccgct cgggccctgc catagcctca
      241 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      541 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      781 cagcgtgagc tatgagaaag cgcccacgcn ttccccgaan ggagaaaggc ggnaaaggta
      841 tncgggtaag ccgcaggggc gnaaacagna anaacgnacn aggnatgttc cagggggaaa
      901 acnncctgga anntttaaag tcctgtcggg gttttncccc cccctgantt nancgtcnat
      961 ttttgngaag gcncgncagg ggggcgggac cctatggnaa aaacgccnag caanncggnc
     1021 cnttttnang gttncgggnc ntttngcggg gcctt
//
