LOCUS       sequence-25578-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E2-5
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(148..168)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(148..166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(187..203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(187..203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(200..222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      401..988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     890..909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gnnngagncn ttaggatcag agatcattaa tggtccgttc aatatctttt ttaattcttt
       61 gttaggcctg gtacctctag agatatcctg cagtggccaa tgcattggtt cgcggccgcc
      121 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
      181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcgggccct gccatagcct
      241 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
      301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
      361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      901 ggtatcttta tagtcctgtc ggggttcgcc ccctctgact gagcgtcgat ttttgtgatg
      961 ctcgtcaggg gggcggagcc tatgnaaaac gccagcaacg cggccttttt acggtnccng
     1021 gcctttggct g
//
