LOCUS       sequence-255809-        8279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Ellwanger K, Becker E, Kienes I, Sowa A, Postma Y, Gloria YC, Weber 
            ANR, Kufer TA
  TITLE     The NLR family pyrin domain containing 11 protein contributes to the
            regulation of inflammatory signalling.
  JOURNAL   J Biol Chem. 2018 Jan 4. pii: RA117.000152. doi: 
            10.1074/jbc.RA117.000152.
  PUBMED    29301940
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Jan 4. pii: RA117.000152. doi: 10.1074/jbc.RA117.000152."
COMMENT     Sequence Label: deltaPYD-NLRP11.MYC
FEATURES             Location/Qualifiers
     source          1..8279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             689..718
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             722..751
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             755..784
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             788..811
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(3636..3653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3642..3866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3912..4340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3999..4018)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4209..4230
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4349..4369)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4354..4683
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4534..4669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4596..4615
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4750..5544
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4804..4823)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5414..5433
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5718..5839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5755..5774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5809..5828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5888..5904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5888..5904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5901..5923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5912..5928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5936..5942,5943..5960,5961..5966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5981..6002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6119..6136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6290..6878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6370..6389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7049..7840,7841..7909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7672..7691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7910..8014)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8089..8108)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttaccatgga gcagaagctg atctccgagg aggacctgaa
      721 cgagcagaag ctgatctccg aggaggacct gaacgagcag aagctgatct ccgaggagga
      781 cctgaacctg gaggtgctgt tccagggacc cggggtacct tcgtcagatg tgttctacat
      841 acttcaatta gcctatgatt ctaccagcta ttattcagca aacaatctca atgtgttcct
      901 gatgggagag agagcatctg gaaaaactat tgttataaat ctggctgtgt tgaggtggat
      961 caagggtgag atgtggcaga acatgatctc gtacgtcgtt cacctcactt ctcacgaaat
     1021 aaaccagatg accaacagca gcttggctga gctaatcgcc aaggactggc ctgacggcca
     1081 ggctcccatt gcagacatcc tgtctgatcc caagaaactc cttttcatcc tcgaggactt
     1141 ggacaacata agattcgagt taaatgtcaa tgaaagtgct ttgtgtagta acagcaccca
     1201 gaaagttccc attccagttc tcctggtcag tttgctgaag agaaaaatgg ctccaggctg
     1261 ctggttcctc atctcctcaa ggcccacacg tgggaataat gtaaaaacgt tcttgaaaga
     1321 ggtagattgc tgcacgacct tgcagctgtc gaatgggaag agggagatat attttaactc
     1381 tttctttaaa gaccgccaga gggcgtcggc agccctccag cttgtacatg aggatgaaat
     1441 actcgtgggt ctgtgccgag tcgccatctt atgctggatc acgtgtactg tcctgaagcg
     1501 gcagatggac aaggggcgtg acttccagct ctgctgccaa acacccactg atctacatgc
     1561 ccactttctt gctgatgcgt tgacatcaga ggctggactt actgccaatc agtatcacct
     1621 aggtctccta aaacgtctgt gtttgctggc tgcaggagga ctgtttctga gcaccctgaa
     1681 tttcagtggt gaagacctca gatgtgttgg gtttactgag gctgatgtct ctgtgttgca
     1741 ggccgcgaat attcttttgc cgagcaacac tcataaagac cgttacaagt tcatacactt
     1801 gaacgtccag gagttttgta cagccattgc atttctgatg gcagtaccca actatctgat
     1861 cccctcaggc agcagagagt ataaagagaa gagagaacaa tactctgact ttaatcaagt
     1921 gtttactttc atttttggtc ttctaaatgc aaacaggaga aagattcttg agacatcctt
     1981 tggataccag ctaccgatgg tagacagctt caagtggtac tcggtgggat acatgaaaca
     2041 tttggaccgt gacccggaaa agttgacgca ccatatgcct ttgttttact gtctctatga
     2101 gaatcgggaa gaagaatttg tgaagacgat tgtggatgct ctcatggagg ttacagttta
     2161 ccttcaatca gacaaggata tgatggtctc attatactgt ctggattact gctgtcacct
     2221 gaggacactt aagttgagcg ttcagcgcat ctttcaaaac aaagagccac ttataaggcc
     2281 aactgctagt caaatgaaga gccttgtcta ctggagagag atctgctctc ttttttatac
     2341 aatggagagc ctccgggagc tgcatatctt tgacaatgac cttaatggta tttcagaaag
     2401 gattctgtct aaagccctgg agcattctag ctgtaaactt cgcacactca agttgtccta
     2461 tgtctcgact gcttctggtt ttgaagactt actcaaggct ttggctcgta atcggagcct
     2521 gacatacctg agtatcaact gtacgtccat ttccctaaat atgttttcac ttctgcatga
     2581 catcctgcac gagcccacat gccaaataag tcatctgagc ttgatgaaat gtgatttgcg
     2641 agccagcgaa tgcgaagaaa tcgcctctct cctcatcagt ggcgggagtc tgagaaaact
     2701 gaccttatcc agcaatccgc tgaggagcga cgggatgaac atactgtgtg atgccttgct
     2761 tcatcccaac tgcactctta tatcactggt gttagtcttc tgctgtctca ctgaaaattg
     2821 ctgcagcgcc cttggaagag tgcttctgtt cagcccaact ctaagacaac tagacctgtg
     2881 tgtgaatcgc ttaaaaaatt acggagtgtt gcatgtgacg tttcccttgc tgtttccaac
     2941 ctgtcagtta gaggagcttc atctgtctgg ctgtttcttt agcagcgata tctgtcaata
     3001 tattgccata gttattgcta ctaatgaaaa actgaggagc ctggagattg ggagcaacaa
     3061 aatagaagat gcaggaatgc agctgctatg tggtggtttg agacatccca actgcatgtt
     3121 ggtgaatatt gggctagaag agtgcatgtt aaccagtgcc tgctgtcgat ctcttgcctc
     3181 tgttcttacc accaacaaaa cactagaaag actcaacttg cttcaaaatc acttgggcaa
     3241 tgatggagtt gcaaaacttc ttgagagctt gatcagccca gattgtgtac ttaaggtagt
     3301 tgggcttcca ttaactggcc tgaacacaca aacccagcag ttgctgatga ctgtaaagga
     3361 aagaaaaccc agtttgatct ttctgtctga aacttggtct ttaaaggaag gcagagaaat
     3421 tggtgtgaca cctgcttctc agccaggttc aataatacct aattctaatt tggattacat
     3481 gtttttcaaa tttcccagaa tgtctgcagc catgagaacg tcaaatacag catctaggca
     3541 acccctttga ggatccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct
     3601 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3661 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3721 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3781 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3841 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3901 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3961 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4021 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4081 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4141 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4201 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4261 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4321 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4501 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4561 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4621 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4681 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4741 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4801 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4861 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4921 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4981 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5041 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5101 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5161 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5221 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5281 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5341 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5401 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5461 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5521 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5581 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5641 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5701 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5761 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5821 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5881 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5941 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6001 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6061 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8041 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     8101 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8161 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     8221 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
