LOCUS       sequence-25581-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E2-6
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(113..316)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(142..162)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        317..620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cnccgggnac gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccggagcttg
       61 agctcgagat ctgagtccgg tagcgctagc ggatctgacg gttcactaaa ccagctctgc
      121 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      181 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
      241 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
      301 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
      361 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
      421 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
      481 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
      541 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
      601 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
      661 ttgattacta ttaataacta atgcatggcg gtaatacggt tatccacaac ggaanagaaa
      721 nccnnaaaaa aantttgnna naaaagggcc acnaatggcc ngaaccctaa aaaggccncg
      781 tngctgggtt tttccatagg ctccnccccc tgacaa
//
