LOCUS       sequence-255817-        9996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes codon optimized HA tagged FAM208A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pcDNA3.1 HA-FAM208A
FEATURES             Location/Qualifiers
     source          1..9996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             930..956
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5587..5604)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5593..5817
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5863..6291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5950..5969)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6160..6181
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6300..6320)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6305..6634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6485..6620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6547..6566
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6701..7495
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6755..6774)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7365..7384
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7669..7790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7706..7725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7760..7779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7839..7855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7839..7855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7852..7874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7863..7879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7887..7893,7894..7911,7912..7917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7932..7953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8070..8087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8241..8829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8321..8340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9000..9791,9792..9860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9623..9642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9861..9965)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga gccgccatgt acccatacga tgttccagat tacgctggat
      961 cagcaggttc agcaggatcc atggcaacgg ctgttgagac agaagcgtgc cagcctaccg
     1021 atgccagctg ggagtcaggg ggcgggggtg atgatgaaat gaaacaagcc ctgccggagc
     1081 tcgaatcctc ccagcagaac gggggtggtg gaggtcttaa tatcgcagag ccttctggtg
     1141 gggcaggccg cgaggaaaac gcaggggccg aagctgctca gagtctttca catgagcagc
     1201 cacaagacag ttctgaagcg ggggcagctg ccttgccaag aggaccagag gaacccgaaa
     1261 gaccagtgcg cagaagtttt cagataccca gaaaaagcag ggagaaaaag gctctgtttc
     1321 aacctcttac acccggctca agggaatttg aagatgttgt taacatcttg cactcatcat
     1381 acttggaacc aacatccgtc acaaatttta actaccggag ggcctgtttg gtacataatg
     1441 agctcttgga aaaggagttc actgagaaga gaagagaact caaattcgat ggacgattgg
     1501 ataaggaact gagcgaatcc tacgctttct tgatggttga tcggtatcaa gtacagacca
     1561 tctgtgagaa ggggctgcac gtggggcaga gtaagataac catacttggt tctcctagta
     1621 tgggggtgta tctttcaagg tatgcggacc tccttcaggc gaaccccctc gacacaggcg
     1681 ccatgggtga cgttgtcatt tttaaaataa tgaagggaaa gattaaatct atctatgatc
     1741 caatgggcgt taaaagcctt gagtcaatgc tcaataagtc cgcgttggac ccgaccccta
     1801 agcatgaatg ccacgtatcc aaaaacgcaa ataggatcac ttctctgctg gcctatcggg
     1861 catacgaact gacacagtac tacttctatg aatatggctt cgatgaactg cgacggcgac
     1921 cgcgacatgt gtgcccctac gcagtagttt cttttacata taaagatgat attcagacgc
     1981 cgaagtttgt cccgagtagc agatcaaata gcttcaatac ggatcgcaat atagataagt
     2041 ataattacac gctctggaag ggacagctcc ttaataaagg aaagttgctc tgctacatta
     2101 gtctccgatc cgcaacacga gcatttctgc cgatcaaact tcctgagaaa ttggacgttg
     2161 agacggttat gtcaattgac caccttaagc agaagattcc ccccgctttg ttttacaagg
     2221 agacgtatct cggaccaaat gaagtgctta aaaatggaat gtactgcagt ttgtatgagg
     2281 tggttgaaaa aactcgcata ggttcaaaca tggagtctct gctccaaaag ctggatagag
     2341 aaaagctggt actcgtaaag ccccttggtg accgaggcta cctttttctg ttgtcaccgt
     2401 atcaaatggt gcctccatac gagtaccaaa cggcgaaatc aagggtactc catgcacttt
     2461 ttttgtttca agaaccacga tctatcgtaa catctcagaa aggctccacc aatgcagcac
     2521 cgcaagagag gcatgaaagc atgccggacg tgctcaagat agctcagttt ctccagttta
     2581 gtttgatcca gtgcaggaag gaatttaaga atatttccgc gatcaatttt catagtgtgg
     2641 ttgagaaata tgttagcgag ttttttaaac ggggttttgg ttcaggcaaa cgagagttta
     2701 tcatgttccc atatgatagt agacttgacg ataagaagtt cctgtacagt gcccctagaa
     2761 ataaaagcca tattgatacg tgcctgcatg cctatatttt ccgccctgag gtataccagc
     2821 tgcctatatg caagctcaaa gagttgttcg aggagaacag aaaattgcaa cagttcagtc
     2881 cattgagtga ttacgaaggg caggaagaag agatgaatgg aacgaaaatg aaattcggaa
     2941 agcgaaataa ttcacgcgga gaggctatca ttagtgggaa gcagcgctcc agtcattcct
     3001 tggactacga taaggaccgg gtgaaggagt tgatcaacct tatacaatgt agaaagaaat
     3061 cagtcggggg agacagcgac actgaggaca tgcgaagtaa aactgtactc aaacgcaaac
     3121 tagaggacct cccagagaat atgcggaaac ttgctaaaac atcaaacttg agtgaaaact
     3181 gtcatctgta tgaagaaagc ccccagccca tcggttccct cggacacgat gctgatctta
     3241 ggcggcagca gcaggatacg tgtaactccg gcattgctga catacacagg ttgttcaatt
     3301 ggctttctga gacactggcg aatgccaggc actccgacgc aagtctcacg gatacagtga
     3361 ataaagcgct gggcctttct acggatgatg cctacgagga gcttcggcag aaacacgagt
     3421 atgagttgaa tagtactcct gataaaaaag attatgagca gcccacttgt gctaaggtcg
     3481 aaaacgcgca gttcaaagga acccaatctc tcctgctcga agtggacgcc acgagcaagt
     3541 actcagtcgc gatctctaca agtgaagtgg gaactgacca taaacttcac ctcaaagaag
     3601 accccaatct gatttccgtc aacaactttg aagactgcag cctgtgtcca agcgtaccaa
     3661 tagagcacgg gttccgcaga caacagtcca aatcaaacaa cgtagaggaa acagaaatac
     3721 actggaaact gattcctatc accgggggta acgctcggag tcctgaggac cagcttggga
     3781 agcatggcga aaaacaaacg cccggcatga agtcccctga agaacaattg gtatgcgttc
     3841 ctccccagga ggccttccct aacgatccga gagtcattaa tcgccaacga agctccgatt
     3901 atcagtttcc gagtagcccc ttcacagata cactgaaggg aaccacagag gatgatgtct
     3961 tgacaggaca ggttgaagaa cagtgcgtac cagcagcaga ggcggagccc cccgctgtat
     4021 ctgaaacgac cgagagaacc gtgcttggag agtacaacct tttctcccgc aagatcgagg
     4081 agattctgaa gcaaaaaaac gtgtcttatg tgtccactgt aagcactcca atcttttcta
     4141 ctcaagaaaa gatgaaaagg cttagcgaat ttatatattc caaaacgtca aaggctgggg
     4201 ttcaagagtt cgtcgatggg ctccatgaga aattgaacac gattattata aaggcgtccg
     4261 ctaaaggtgg taatctccct cctgtgagcc cgaacgattc cggtgcaaag attgcatcaa
     4321 acccgcttga acgacacgtc atcccggtca gttcatcaga tttcaacaac aaacacctgt
     4381 tggagccttt gtgctctgat ccccttaaag acactaacag cgacgaacaa cacagcacga
     4441 gcgccctcac ggaggtcgaa atgaaccaac cacaacacgc aaccgaactc atggtcacat
     4501 ccgaccacat cgtgcctgga gatatggcta gggagcctgt tgaggagacg actaagagcc
     4561 ccagcgatgt caatatctct gcacaacctg ccctctcaaa ttttataagc cagttggagc
     4621 cggaagtatt taactcactc gttaaaataa tgaaagatgt gcagaagaac acggtcaaat
     4681 tttatataca tgaggaagag gaaagcgtgc tgtgtaaaga gatcaaggag taccttatta
     4741 agcttggtaa caccgagtgc catcctgagc aattccttga aagacgatcc aagctcgaca
     4801 agttgttgat tattattcag aatgaagaca tcgcgggttt tatacacaag atacctggct
     4861 tggttacatt gaagaaactt ccctgcgtca gttttgcggg ggtggatagc ctggacgatg
     4921 tgaaaaatca cacctataac gaactgttcg tatctggcgg ctttattgtg agtgacgaat
     4981 ctatcttgaa tcccgaggtg gtcacggtcg aaaatctgaa gaattttctt accttcttgg
     5041 aagaacttag cacacctgaa gggaaatggc agtggaaggt tcactgcaag ttccaaaaaa
     5101 aacttaagga actgggcagg ttgaacgcca aagcgctctc actgttgacc ctcctgaatg
     5161 tatatcaaaa gaaacatctt gttgagatat tgagttacca taattgtgat agccaaacca
     5221 gaaatgctcc agaattggat tgcttgatta gacttcaagc ccaaaacatc caacaacggc
     5281 acatagtttt cttgacggaa aaaaatatta aaatgttgag ctcatacacc gacaatggaa
     5341 tagtcgtagc tactgcagaa gactttatgc aaaacttcaa gaatctcgtg ggttatcata
     5401 atagtataac cgaagagaac cttcctcaac tgggtgcgaa tgaaaatctt gagtcacaaa
     5461 gtgatgctgt acttacgttg acccctctcg aactgggcgt aggaatctca caacattgag
     5521 aattccacca cactggacta gtggatccga gctcggtacc aagcttaagt ttaaaccgct
     5581 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5641 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5701 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     5761 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     5821 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     5881 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5941 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6001 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6061 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6121 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6181 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6241 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     6301 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6361 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6421 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6541 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     6601 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     6661 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     6721 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     6781 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     6841 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     6901 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     6961 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7021 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7081 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7141 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7201 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     7261 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7321 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     7381 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     7441 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     7501 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     7561 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7621 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     7801 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7861 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7921 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7981 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8161 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8401 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8641 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8821 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8881 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8941 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9001 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9061 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9121 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9181 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9241 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9301 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9361 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9421 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9481 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9541 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9601 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9661 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9721 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9781 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9841 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9901 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9961 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
