LOCUS       sequence-25582-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6-shR-E2-6
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(143..163)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(143..161)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(182..198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(182..198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(195..217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ggngagacag cgttcagaga ctcttggatc ttctactgct gcttttttat tctttgttag
       61 gcctggtacc tctagagata tcctgcagtg gccaatgcat tggttcgcgg ccgccaccgc
      121 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
      181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcgg gccctgccat agcctcaggn
      241 tactcatata tacnttagat tgatttaaan cttcattttt aatttaaaag gatctaggng
      301 aaaacccttt tgaaaatccc atgaccaaan cccntaacgn gagntttcgt nccctgggcg
      361 ncanancccg tanaaaaaat caaaggacnt cntggaancc nttttttcgg ccgnaancng
      421 cngctggnaa aaaaaaaaac cccnctacna cggggggttn tttnccggaa naaanntacc
      481 cactcntttt ccnaagnnaa ngggntncnc nnanngccga aaaccaaaaa ntggtnttct
      541 aagggngccn nnnntnggcc cccccntnna anaaactnnn gnanccnccc aaaaaccccc
      601 gcnnggnaaa accgttacaa aagggngncc nccnngggga aaaaaacngg gntttcccgg
      661 ggggngnccc anaagacgat antcccggat aaggcgcagc ggtcgggctg aacggggggt
      721 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      781 gagctatgag aaagcgccac gcttcccgaa gggagaaggc ggacaggtat ccggtagcgg
      841 cagggtcgga acaggagagc gcacgaggga gcttccaggg nactgatagc gtgnacggga
      901 ggtggganga ggggnagagg ggaagggggg ggggnngggg ggggg
//
