LOCUS       sequence-255840-       10460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium T-DNA overexpression cassette comprising constitutive 
            TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene
            in F. solani genome. Replace eYFP w/ XhoI BamHI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Nielsen MR, Holzwarth AKR, Brew E, Chrapkova N, Kaniki SEK, 
            Kastaniegaard K, Sorensen T, Westphal KR, Wimmer R, Sondergaard TE, 
            Sorensen JL
  TITLE     A new vector system for targeted integration and overexpression of 
            genes in the crop pathogen Fusarium solani.
  JOURNAL   Fungal Biol Biotechnol. 2019 Dec 11;6:25. doi: 
            10.1186/s40694-019-0089-2. eCollection 2019.
  PUBMED    31890232
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2019-12-11"; volume: "6:25. doi"; pages: " 
            10.1186/s40694-019-0089-2. eCollection 2019"
COMMENT     Sequence Label: pSHUT4-eYFP
FEATURES             Location/Qualifiers
     source          1..10460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(150..1298)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(1597..2391)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             complement(2705..3508)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3509..3729)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      4577..5208
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    5347..5371
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6485..7279)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6485..7279)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6596..6615)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7206..7225
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             join(8414..8416,8417..9130)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8456..8477)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8717..8736)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9064..9085
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      9142..9394
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    join(10460,1..24)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
       61 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagca
      121 gatccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
      181 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
      241 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
      301 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
      361 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
      421 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
      481 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
      541 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
      601 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
      661 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
      721 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
      781 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
      841 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
      901 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
      961 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
     1021 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
     1081 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
     1141 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
     1201 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
     1261 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
     1321 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
     1381 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
     1441 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
     1501 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
     1561 tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat ccagtaaaat
     1621 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
     1681 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
     1741 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
     1801 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
     1861 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
     1921 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
     1981 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
     2041 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
     2101 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
     2161 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
     2221 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
     2281 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
     2341 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
     2401 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
     2461 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
     2521 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta
     2581 tgggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatgggt aataactgat
     2641 ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag
     2701 ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac
     2761 gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa
     2821 tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc
     2881 ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc
     2941 cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt
     3001 caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta
     3061 acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa
     3121 caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt
     3181 atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt
     3241 cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca
     3301 tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg
     3361 cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg
     3421 tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag
     3481 cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc
     3541 tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta
     3601 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
     3661 aatcaaaaaa atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
     3721 tgaattgaaa agctagctta tcgatgataa gctgtcaaag atgagaatta attccacgga
     3781 ctatagacta tactagatac tccgtctact gtacgataca cttccgctca ggtccttgtc
     3841 ctttaacgag gccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg
     3901 tgatcctggc cttttgctca tgattacgaa ttcatcacgt gctataaaaa taattataat
     3961 ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga
     4021 aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact
     4081 accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg
     4141 tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg
     4201 tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt
     4261 tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct
     4321 ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc
     4381 ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacgaatt cgagccaagt
     4441 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     4501 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatgcgtac gcctgggggg
     4561 atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc
     4621 gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt
     4681 ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa
     4741 atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg
     4801 tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc
     4861 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
     4921 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
     4981 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
     5041 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
     5101 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
     5161 tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgc aaaggcgctc
     5221 ggtcttgcct tgctcgtcgg atctggaacc ctgtggttgg catgaacaaa caaatggacg
     5281 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
     5341 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga
     5401 aacgacaatc tgatcatgag cgcaccaagt ccataagcca caggcgatcc tcctcgtgtt
     5461 gaggcagagg cccgccgaag accaaggacg ggagcaggga acccacatcc cggttgcagt
     5521 agagtcaggt cgaggccaag ataaggctgg ctcacaaact ctgtccatgt tcgaagccta
     5581 gtgctcacga actggtgtcc aacggtacag tacatgtgtg tacctgcgga agccatggaa
     5641 acatgtcgtc gaaaacacca ctcgtacctg aaacaaggca gccatgagaa atccttcttc
     5701 tccatccatg ggacgtctgt gctgggtcat cccttggtcc atgcagagtc tgatgcatcg
     5761 tagacgcata tgaatatatc ccagggagga gaggagacga aaaaacaatt cagccccagc
     5821 atcagcatgc gaaccagtca tcttgccact ggagtcatcc gaaacccacc gatcatgtct
     5881 tgatctggcc atgcaccgag gactctatcg taagctcggc ttttactgaa tattgttgac
     5941 gccgaccagt ccaatttcca caaggcctcc tcaacggcgg ctaggagaac cgatagacgt
     6001 gccaagtgct catctagtgc tcacgatcca ggctgacgca atgcctgaac cgcttgtctt
     6061 atgatgggcc taaacccaag tgccatggga tgcttgtcga ttccacacgg ctgggtttga
     6121 ccaccaggcc aggccgatcg gcaaacaaat ccctctccgc ggtggctggc tagggtccta
     6181 tttcgaggct catggggcca aagtcgttat taatgcctca gcactagtca tgcaacatgc
     6241 atgtactgtc tgatgtatta agagtatagg ggtcaacaac tagagtgtct gacaggtata
     6301 tctagcagtt tgatttgacc attcttttat taaaacactg taacccgcaa cgaagaactc
     6361 ctccgcttca agtcaagaat gcgcatatgt gcgtaaaatc gacaagcccg gcgcattcca
     6421 ccgagcaggg tattgccttt ggactttacg tgcgcagagg agcctgaatg ttgagtggaa
     6481 tgattcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     6541 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagttct tcagcaatat
     6601 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     6661 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     6721 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
     6781 gcgcgagccc ctgatgttct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     6841 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     6901 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     6961 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     7021 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     7081 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     7141 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     7201 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     7261 atccatcttg ttcaatcatt ttgaagattg ggttcctctt tgagagagtt aatagacttg
     7321 aattgaggaa tggaaatcgg gctgagctca ctgctttata tgtgagaaaa ggtgatgagc
     7381 aggtggtaag aggcagattt ttgtggtcgg tgctgtgttt tgttgggttt gagctaggtg
     7441 gggtgaggga ggggcaagct aaatagctac cagccctgag gacgtcacag agttttagta
     7501 gttctttttc tgccatcgga catgcagctc acgcctagac ccaattacac cctttgcgcc
     7561 ctcccacaca tccggccggt tggatcattg tcagatacgg cagagaaatc gcaacctcgg
     7621 ccaagggtag ccacgattcg aaccggtgat tttcactgtg gaccagacag gcgccactcg
     7681 gccgggccac agctgcttgg gtgttgaccg ggagcggacc aattaaggac tcgaacgacc
     7741 gcggggttca aatgcaaaca agtacaacac gcagcaaacg aagcagccca ccactgcgtt
     7801 gatgcccagt ttgactgtcc gaaatccacc ggaaaggtgg aaacatacta tgtaacaatc
     7861 agagggaaga aaaaattttt atcgacgagg caggatagtg actgatggtg gggtcatggt
     7921 cgggtctccg agcgaaagag aaccaaggaa acaagatcaa cgaggttggt gtacccaaaa
     7981 ggccgcagca acaagagtca tcgcccaaaa gtcaacagtc tggaagagac tccgccgtgc
     8041 agattctgcg tcggtcccgc acatgcgtgg tgggggcatt acccctccat gtccaatgat
     8101 aagggcggcg gtcgagggct taagcccgcc cactaattcg ccttctcgct tgcccctcca
     8161 tataaggatt ccccctcctt cccctcccac aacttttttc cttctttctc tcttcgtccg
     8221 catcagtacg tatatctttc ccccatacct cctttcctac tcttcttcca ttcattcaac
     8281 tcttctcctt actgacatct gttttgctca gtacctctac gcgatcagcc gtagtatctg
     8341 agcaagcttc tctacagaat ctttctagta tcttacaaag aactacaaag ttcgcaccac
     8401 cttcaaagga tccatgggca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     8461 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     8521 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     8581 ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga
     8641 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     8701 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     8761 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     8821 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     8881 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     8941 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     9001 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     9061 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     9121 gtacaagtaa taactcgagc cgatcgttca aacatttggc aataaagttt cttaagattg
     9181 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     9241 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     9301 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     9361 ttatcgcgcg cggtgtcatc tatgttacta gatcgggccc gctgaggact taatactcgt
     9421 ctctgtcagt gtattaattt cgtcctgaaa aggtccacat gaaattcaaa gcgagatcta
     9481 aacagaaaaa agagctccat actgacatgg gcttctccgt tctcggtcca cgcttcgtcg
     9541 gcggtttgga gctatcctac accaagtgga gaggcagcgc cgagctcaga tggacatgac
     9601 gttaaatcga ccatcgcttg cgacggcgcc gtgtctgcgt gtgtagctcg ggcggccacg
     9661 agtgccggca tccagatgat ggatggcaga ccagggcagt cgtgcttcac caagtgagac
     9721 aaaaacgtcc gtggtctcgg ccccttaggg gtggtgtttt gtgtggtggg gggaatgcgg
     9781 aacaacaaaa tcaaatcttg atggagcctc aagagccgca atctcccaaa aacacaacaa
     9841 aggaataaag aagaagttat tcttcccggc acgtaatgta cgtgatggcg tgcagaaagt
     9901 ccttgcatcg atctcccgta atgcacaagg atatcgagcg gatctcctat gcaatgttgt
     9961 gcagaacctg tacgcaagac aagacaagca acatgatagc tcggtccgca caagatggtt
    10021 tgcattgcca accaatggcc acaaatgcaa caacaaaaac atccgtccgt agaccgacac
    10081 cgaggttgac gcgtcgcgtc gtgatcgtcc acaacacact cacactctcg gcggactatt
    10141 ttagagtttt ccggggtgcg attggcgcac gagattcgag ctgaagagct gctgaatgcg
    10201 gcgtagattg gaattcgtgg catcaacgtc gtgactggga aaaccctggc gttacccaac
    10261 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    10321 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgccgc tgttgcccgt
    10381 ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa cgtccgcaat gtgttattaa
    10441 gttgtctaag cgtcaatttg
//
