LOCUS       sequence-255843-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating SPARK NanoLuc-o-beta-2AR transmembrane component 
            lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Kim CK, Cho KF, Kim MW, Ting AY
  TITLE     Luciferase-LOV BRET enables versatile and specific transcriptional 
            readout of cellular protein-protein interactions.
  JOURNAL   eLife. 2019 Apr 3;8. pii: 43826. doi: 10.7554/eLife.43826.
  PUBMED    30942168
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-04-3"; volume: "8. pii: 43826. doi"; pages: " 
            10.7554/eLife.43826"
COMMENT     Sequence Label: pLX208-gLuc
            sp-NanoLuc-HA-o-beta-2AR-TS-eLOV-TEVcs-...
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1843..1960
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2077..2380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2381..2584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2535..2555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2607..2616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2664..3173
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             3174..3200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3213..3251
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             4998..5018
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             5031..5054
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5055..5492
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5493..5837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    5853..6441
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5906..5926)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6324..6335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6513..6746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6824..6945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6861..6880)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6915..6934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6985..7120
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7140..7159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7141..7159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7169..7186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7169..7185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7178..7200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7327..7782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7414..7433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7624..7645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7808..7912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7913..7981,7982..8773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8131..8150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8944..9532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9433..9452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9686..9703
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9820..9841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9856..9861,9862..9879,9880..9886)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9894..9910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9899..9921
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9918..9934
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9918..9934
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9953..9973
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9955..9973
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg agagcgctgc cgtctggacc gatggctgtg tggaagtgct
     1741 cgccgatagt ggaaacagac gccccagcac tcgtcctagg gcaaaggatc tgcagtaatg
     1801 agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     1861 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1921 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1981 cagagatcca ctttggcgcc ggctcgaggg ggcccggggt tattaatagt aatcaattac
     2041 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2101 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2161 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2221 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2281 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2341 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2401 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2461 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2521 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2581 agctctctgg ctaactgtcg ggatccgcca ccatgggagt caaagttctg tttgccctga
     2641 tctgcatcgc tgtggccgag gccgtcttca cactcgaaga tttcgttggg gactggcgac
     2701 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
     2761 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
     2821 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
     2881 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
     2941 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
     3001 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
     3061 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
     3121 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgtatccgt
     3181 acgacgtacc agactacgca aacggaacag agggcccaaa cttttacgtt cccttctcca
     3241 ataagactgg ggtcgtgaga agcccatttg aggcgcctca atactacctt gctgagccgt
     3301 ggcagttttc tatgctcgct gcttacatgt tcttgctgat catgctgggg ttccctatca
     3361 atttcctgac gctgtacgtt atagcaaagt tcgaacgcct ccaaaccgtg ttgaactaca
     3421 tactccttaa cctcgcggtt gccgacctct tcatggtttt cgggggtttc accaccaccc
     3481 tctacacctc ccttcacggc tacttcgtgt tcggccctac cggatgcaat ctggaaggct
     3541 ttttcgcaac gctggggggg gagattgccc tttggagcct ggtggtcttg gccatagaga
     3601 ggtacgtggt ggtcacatcc ccattcaagt accagagttt gcttacaaag aacaaggcta
     3661 tcatgggggt cgccttcaca tgggtgatgg cgctggcttg cgctgcccca ccgctggtag
     3721 gctggtcccg gtatattccg gagggaatgc agtgcagttg tgggatcgac tactacaccc
     3781 cacacgaaga gactaacaac gagtcttttg tgatttatat gttcgtggtc cacttcatca
     3841 tccccctgat agtgatcttt ttctgttacg gcagggtgtt ccaggtcgcc aaaaggcagc
     3901 tccagaagat cgacaaaagc gaaggccgct ttcacagccc caatcttgga caggttgaac
     3961 aggacggcag gtcagggcac gggctgcgac gcagttctaa gttctgcctg aaggaacata
     4021 aggccttgag aatggtgatc atcatggtaa tcgccttcct gatatgctgg cttccatacg
     4081 ctggcgtggc tttttatata ttcacgcacc aggggtcaga ttttgggcct atctttatga
     4141 ccatacctgc tttcttcgct aagacgagtg cggtgtataa cccagtgata tacatcatga
     4201 tgaacaaaca attcagaatt gccttccagg aattgctctg tctcagacgc agctcttcca
     4261 aagcgtacgg aaatggctat tcatctaaca gcaacggaaa gactgattat atgggcgaag
     4321 ccagtggctg ccagctgggc caggaaaaag agagcgagcg gctttgtgaa gatcccccag
     4381 gcactgagag cttcgtgaat tgtcagggaa cagttccgag tctctctctt gattcacagg
     4441 gacgcaattg ctctaccaac gacagccccc tggagacttc ccaggtcgct ccggccaaga
     4501 gcaggatcac cagcgagggc gagtacatcc ccctggacca gatcgacatc aacgtgggtg
     4561 gagctagcgg cagccgggcg accaccctgg aacgtattga aaagagcttt gtgattaccg
     4621 atccgcgtct gccggataac ccgattattt ttgtgagcga tagctttctg cagctgaccg
     4681 aatatagccg tgaagaaatt ctgggccgta actgccgttt tctgcagggc ccggaaaccg
     4741 atcgtgcgac cgtgcgtaaa attcgtgatg cgattgataa ccagaccgaa gtgaccgtgc
     4801 agctgattaa ctataccaaa agcggcaaaa aattttggaa cctgtttcat ctgcagccga
     4861 tgcgtgatca gaaaggcgat gtgcagtatt ttattggcgt gcagctggat ggtaccgaac
     4921 gtgtgcgtga tgcggcggaa cgtgaagctg tgatgctggt caagaaaacc gcggaggaaa
     4981 tcgacgaagc cgctaaggaa aatctctact tccagatggg cggaggatcc gactacaagg
     5041 atgacgacga taagaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
     5101 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
     5161 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
     5221 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
     5281 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     5341 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     5401 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     5461 gtaacaaagg tcaaagacag ttgactgtat cggccaattt taatcaaagt gggaatattg
     5521 ctgatagctc attgtccttc actttcacta acagtagcaa cggtccgaac ctcataacaa
     5581 ctcaaacaaa ttctcaagcg ctttcacaac caattgcctc ctctaacgtt catgataact
     5641 tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg taataattca aaaccactgt
     5701 cacctggttg gacggaccaa actgcgtata acgcgtttgg aatcactaca gggatgttta
     5761 ataccactac aatggatgat gtatataact atctattcga tgatgaagat accccaccaa
     5821 acccaaaaaa agagtagacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     5881 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5941 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6001 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6061 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6121 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6181 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6241 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6301 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6361 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6421 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     6481 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6541 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6721 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     6781 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6841 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6901 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     6961 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7021 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7081 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7141 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7201 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7261 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7321 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7381 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7441 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7501 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7561 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7621 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7681 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7741 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7801 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7861 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7921 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7981 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8041 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8101 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8161 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8221 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8281 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8341 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8401 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8461 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8521 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8581 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8641 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8701 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8761 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9661 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9721 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9781 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9841 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9901 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     9961 ccctcactaa agggaacaaa agctggagct gcaagcttaa
//
